A5084940572- Blood properties and coagulation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Rheology and Fluid Dynamics Studies
- Venous Thromboembolism Diagnosis and Management
- Platelet Disorders and Treatments
- Mechanical Circulatory Support Devices
- Polysaccharides Composition and Applications
- Coronary Interventions and Diagnostics
- 3D Printing in Biomedical Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Erythrocyte Function and Pathophysiology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Acute Myocardial Infarction Research
- Obstructive Sleep Apnea Research
- COVID-19 Clinical Research Studies
- Inflammatory Biomarkers in Disease Prognosis
- Sepsis Diagnosis and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Membrane-based Ion Separation Techniques
- Surfactants and Colloidal Systems
- Acute Ischemic Stroke Management
- Bone Tissue Engineering Materials
- Fluid Dynamics and Heat Transfer
- Fluid Dynamics and Mixing
- Advanced Sensor and Energy Harvesting Materials
Swansea University
2016-2025
Swansea Bay University Health Board
2014-2017
Health and Care Research Wales
2014-2017
Morriston Hospital
2009-2016
Royal Brompton Hospital
2016
Royal Brompton & Harefield NHS Foundation Trust
2016
Cardiff University
2016
University of Wales
2003-2007
Nestlé (Switzerland)
2004
Royal College of Physicians
1975
Background: One of the main challenges for extrusion 3D bioprinting is identification non-synthetic bioinks with suitable rheological properties and biocompatibility. Our aim was to optimize compare printability crystal, fibril blend formulations novel pulp derived nanocellulose assess biocompatibility human nasoseptal chondrocytes. Methods: The crystalline, fibrillated determined by assessing resolution (grid-line assay), post-printing shape fidelity rheology (elasticity, viscosity shear...
Infections are frequent and very undesired occurrences after orthopedic procedures; furthermore, the growing concern caused by rise in antibiotic resistance is progressively dwindling efficacy of such drugs. Artificial bone graft materials could solve some problems associated with gold standard use natural as limited material, pain at donor site rejections if tissue used. We have previously described new acrylate base nanocomposite hydrogels materials. In present paper, we describe...
The influence of a novel, safe antibiofilm therapy on the mechanical properties Pseudomonas aeruginosa and Acinetobacter baumannii biofilms in vitro was characterized. A multiscale approach employing atomic force microscopy (AFM) rheometry used to quantify disruption by therapeutic polymer based low-molecular weight alginate oligosaccharide (OligoG). AFM demonstrated structural alterations exposed OligoG, with significantly lower Young's moduli than untreated biofilms, (149 MPa vs 242 MPa; p...
The host- and bacteria-derived extracellular polysaccharide coating of the lung is a considerable challenge in chronic respiratory disease powerful barrier to effective drug delivery. A low molecular weight 12–15-mer alginate oligosaccharide (OligoG CF-5/20), derived from plant biopolymers, was shown modulate polyanionic components this coating. Molecular modeling Fourier transform infrared spectroscopy demonstrated binding between OligoG CF-5/20 mucins. Ex vivo studies showed induced...
It is shown herein that it possible to control the mechanical and microstructural properties of collagen gels by manipulating temperature in vicinity sol–gel transition; Fractional Maxwell Model also accurately describe rheological behaviour such gels.
Implantable ventricular assist devices (VADs) have proven efficient in advanced heart failure patients as a bridge-to-transplant or destination therapy. However, VAD usage often leads to infection, bleeding, and thrombosis, side effects attributable the damage blood cells plasma proteins. Measuring hemolysis alone does not provide sufficient information understand total damage, research exploring impact of currently available pumps on wider range cell types proteins such von Willebrand...
We present a simple new analytical method for educing the materials' linear viscoelastic properties, over widest range of experimentally accessible frequencies, from step-strain measurement, without need preconceived models nor idealization real measurements. This is achieved by evaluating Fourier transforms raw experimental data describing both time-dependent stress and strain functions. The novel has been implemented into an open access executable “i-Rheo,” enabling its use to broad...
Clinical outcomes from ventricular assist devices (VADs) have improved significantly during recent decades, but bleeding episodes remain a common complication of long-term VAD usage. Greater understanding the effect shear stress in on platelet aggregation, which is influenced by functional activity high molecular weight (HMW) von Willebrand factor (vWF), could provide insight into these complications. However, because rates are difficult to assess, there need for model that enables...
Stroke is the second largest cause of death worldwide. Hypercoagulability a key feature in ischaemic stroke due to development an abnormally dense clot structure but techniques assessing mechanics and quality microstructure have limited clinical use. We previously validated new haemorheological technique using three parameters reflect (Fractal Dimension (d f )) ex-vivo, real-time formation time (T GP ) blood strength (elasticity at gel point (G'GP)). aimed evaluate these novel clotting...
This study presents a numerical model for incipient fibrin-clot formation that captures characteristic rheological and microstructural features of the clot at gel point. Using mesoscale-clustering framework, we evaluate effect concentration or volume fraction branching on fractal dimension, time, viscoelastic properties clots. We show variations in our can reproduce thrombin fibrin In particular, reproduces dimension's dependency trends elasticity gelation time with varying concentrations....
Abstract Background Exercise in healthy individuals is associated with a hypercoagulable phase, leading to temporary increase clot mass and strength, which are controlled by an effective fibrinolytic system. Conversely, people cardiovascular diseases often have reduced pathway, increased abnormal contraction, resulting poorer outcomes. We assessed microstructure, particularly the contractile forces of formation, response two exercise intensities middle-aged/older runners. Methods...
Venous thromboembolism (VTE) is common in cancer patients, and the second commonest cause of death associated with disease. Patients chronic inflammation, such as cancer, have been shown to pathological clot structures modulated mechanical properties. Fractal dimension (df) a new technique which has act marker microstructure properties blood clots, can be performed more readily than current methods scanning electron microscopy (SEM). We measured df 87 consecutive patients newly diagnosed...
We report a study of the microstructural templating role incipient fibrin–thrombin gels by analysis rheological and confocal microscope measurements. Fractal based on spectral dimension is used, for first time, to characterise fibrin gel microstructure in terms internal connectivity networks. A significant correlation found between fractal characteristics network its eventual mature form, confirming that templates ensuing development. an analytical basis this effect which reveals two...
Incipient clot formation in whole blood and fibrin gels was studied by the rheometric techniques of controlled stress parallel superposition (CSPS) small amplitude oscillatory shear (SAOS).The effects unidirectional on incipient microstructure, kinetics elasticity are reported terms fractal dimension (d f ) network, gel network time (T GP elastic modulus, respectively.The results this first haemorheological application CSPS reveal marked sensitivity microstructure to physiologically relevant...
Objective To assess the prognostic and diagnostic value of whole blood impedance aggregometry in patients with sepsis SIRS to compare parameters (platelet count, haemoglobin, haematocrit white cell count). Methods We performed an observational, prospective study acute setting. Platelet function was determined using (multiplate) on admission Emergency Department or Intensive Care Unit at 6 24 hours post admission. count were also determined. Results 106 adult that met criteria included....
Summary This study compared patients with venous thromboembolism ( VTE ) to non‐ using a biomarker of clot microstructure d f and formation time T GP ). was the only marker that identified significant difference P < 0·001) between n = 60) cohorts 69). The ‘abnormal’ microstructures in suggests either inadequate response anticoagulant therapy or presence procoagulant state not detected by other markers coagulation (i.e., I nternational N ormalized R atio). Furthermore, elevated values...