A5002894950- Fatty Acid Research and Health
- Eicosanoids and Hypertension Pharmacology
- French Urban and Social Studies
- Archaeology and Historical Studies
- Historical and Linguistic Studies
- Inflammatory mediators and NSAID effects
- Multiculturalism, Politics, Migration, Gender
- Cholesterol and Lipid Metabolism
- Peroxisome Proliferator-Activated Receptors
- Islamic Studies and History
- Antiplatelet Therapy and Cardiovascular Diseases
- Psychoanalysis and Psychopathology Research
- Lipid metabolism and biosynthesis
- Language, Linguistics, Cultural Analysis
- Antioxidant Activity and Oxidative Stress
- Political and Social Issues
- Advanced Glycation End Products research
- Medieval Philosophy and Theology
- Historical and Literary Studies
- Historical Studies and Socio-cultural Analysis
- Nitric Oxide and Endothelin Effects
- Sphingolipid Metabolism and Signaling
- Health, Medicine and Society
- Linguistics and Discourse Analysis
- Alcohol Consumption and Health Effects
Institut National des Sciences Appliquées de Lyon
2014-2025
Laboratoire de Mécanique des Contacts et des Structures
2024-2025
Université Claude Bernard Lyon 1
2013-2024
Centre National de la Recherche Scientifique
1988-2024
Pontificio Istituto di Studi e d'Islamitica
2023
Pontifical University of the Holy Cross
2023
Laboratoire CarMeN
2011-2022
Inserm
2011-2020
Hospices Civils de Lyon
2007-2018
Université de Pau et des Pays de l'Adour
2015-2017
High-density lipoprotein (HDL) displays multiple atheroprotective activities and is highly heterogeneous in structure, composition, function; the molecular determinants of functions HDL are incompletely understood. Because phospholipids represent a major bioactive lipid component HDL, we characterized phosphosphingolipidome normolipidemic subpopulations related it to functionality.Using an original liquid chromatography-mass spectrometry/mass spectrometry methodology for phospholipid...
Human prostaglandin-endoperoxide H synthase-1 and -2 (hPGHS-1 hPGHS-2) were expressed by transient transfection of COS-1 cells. Microsomes prepared from the transfected cells used to measure rates oxygenation several 18- 20-carbon polyunsaturated fatty acid substrates including eicosapentaenoic, arachidonic, dihomo-gamma-linolenic > alpha-linolenic (delta 9, 12, 15), gamma-linolenic, linoleic acids. Comparisons kcat/Km values indicate that order efficiency is arachidonate...
Unsaturated lysophosphatidylcholines (lysoPtdCho) bound to albumin circulate in blood plasma and seem be a novel transport system for carrying polyunsaturated fatty acids (PUFA) tissues that are rich these acids, such as the brain. The potential of lysoPtdCho significant source PUFA cells has been assessed by comparing their concentration with unsaturated non-esterified (NEFA) albumin. In humans, was 25.9+/-3.1 nmol/ml lysoPtdCho, compared 33.4+/-9.6 NEFA; rats equivalent values 14.2+/-0.6...
We have previously reported that endothelial cells synthesize a cytosol-associated, lipoxygenase-derived metabolite, LOX, which acts as chemorepellant and, in so doing, maintains the vessel wall thromboresistance.In this study we demonstrate LOX is 13hydroxylinoleic acid (13-OH-18:2) derived from linoleic and identical to 13-hydroxy-S-cis,l l-transoctadecadienoic acid, measured by both reverse phase high pressure liquid chromatography gas chromatographyJmass spectrometry.In addition,...
Dietary intake of long-chain n-3 PUFA is now widely advised for public health and in medical practice. However, are highly prone to oxidation, producing potentially deleterious 4-hydroxy-2-alkenals. Even so, the impact consuming oxidized on metabolic oxidative stress inflammation poorly described. We therefore studied such effects hypothesized involvement intestinal absorption 4-hydroxy-2-hexenal (4-HHE), an end-product. In vivo, four groups mice were fed 8 weeks high-fat diets containing...
The terminal adipose differentiation of Ob1771 cells, characterized by glycerol-3-phosphate dehydrogenase activity and triacylglycerol accumulation, was studied in serum-free hormone-supplemented medium containing growth hormone, tri-iodothyronine, insulin, transferrin fetuin. Arachidonic acid able to substitute for a crude adipogenic fraction isolated from fetal bovine serum but not hormone or tri-iodothyronine. also increase rapid dramatic manner cyclic AMP production; moreover it amplify...
Numerous oxidants are produced as by-products of aerobic cell metabolism, and there is growing evidence that they play key roles in the pathogenesis insulin resistance. Under conditions oxidative stress, lipid peroxidation ω6-polyunsaturated fatty acids leads to production 4-hydroxy-2-nonenal (4-HNE). Several lines suggest 4-HNE could be involved pathophysiology metabolic diseases; therefore, this study we assessed direct effects on skeletal muscle sensitivity. Gastrocnemius L6 cells were...
Neuroinflammatory processes are considered a double-edged sword, having both protective and detrimental effects in the brain. Microglia, brain's resident innate immune cells, key component of neuroinflammatory response. There is growing interest developing drugs to target microglia control processes. In this regard, docosahexaenoic acid (DHA), n-3 polyunsaturated fatty acid, promising molecule regulate pro-inflammatory cytokine production. Several works reported that bioavailability DHA...
The uptake and metabolism of [3H]docosahexaenoic acid (DHA) esterified at the sn-2 position lysophosphatidylcholine (lysoPC DHA) in unesterified form, both bound to albumin, was studied 20-day-old rats. LysoPC DHA preferentially recovered brain (4-5% injected radioactivity) over form (0.3-0.4%). Conversely, lysoPC taken up less than or same extent as by liver, heart, kidney. In brain, mainly phosphatidylethanolamine whether used, although from phosphatidylcholine after 2.5 min. labeled...
The amount and distribution of [13C]docosahexaenoic acid (DHA) in plasma, platelet, erythrocyte lipid classes were followed as a function time (1 to 72 h) young adults after ingestion single dose [13C]DHA esterified phosphatidylcholine (PC), using gas chromatography combustion–isotope ratio mass spectrometry. first appeared plasma non-esterified fatty acids (NEFA) triglycerides (TG), with maximal appearance at 6 h further decline, then being delayed 3-fold compared ingested triglycerides....
Abstract: The aim of the present study was to investigate whether unsaturated 2‐acyl‐lysophosphatidylcholine bound plasma albumin is a relevant delivery form fatty acids developing brain. Twenty‐day‐old rats were perfused for 30 s with labeled palmitic, oleic, linoleic, and arachidonic in either their unesterified or esterified on choline acid moieties. Both forms albumin. Incorporation brain lipid classes followed within 1 h. uptake reached plateau at 5–155 mim maximal (0.45% dose)....
Our study aimed to establish the complete structure of main dihydroxy conjugated triene issued from lipoxygenation (soybean enzyme) docosahexaenoic acid, named PDX, an isomer protectin/neuroprotectin D1 (PD1/NPD1) described by Bazan and Serhan. NMR approaches other chemical characterization (e.g. GC-MS, HPLC LC-MS/MS) indicated that PDX is 10(S),17(S)-dihydroxy-docosahexa-4Z,7Z,11E,13Z,15E,19Z-enoic acid. The use (18)O(2) mass spectrometry showed a double product. Its differs PD1, with E,Z,E...
Dietary intake of n-3 polyunsaturated fatty acids (PUFA) is primarily recognized to protect against cardiovascular diseases, cognitive dysfunctions and the onset obesity associated metabolic disorders. However, some their properties such as bioavailability can depend on chemical carriers. The objective our study was test hypothesis that nature PUFA carrier results in different effects related adiposity, oxidative stress inflammation.4 groups C57BL/6 mice were fed for 8 weeks low fat (LF)...
Stroke is a devastating neurological disorder that characterized by the sudden disruption of blood flow to brain. Lipids are essential components brain structure and function play pivotal roles in stroke pathophysiology. Dysregulation lipid signaling pathways modulates key cellular processes such as apoptosis, inflammation, oxidative stress, exacerbating ischemic injury. In present review, we summarize lipids pathology different models (cell cultures, animal, human studies). Additionally,...