A5056012034- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Probiotics and Fermented Foods
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Resistance in Staphylococcus
- HIV/AIDS oral health manifestations
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Nail Diseases and Treatments
- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Oral microbiology and periodontitis research
- Infectious Diseases and Mycology
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Helicobacter pylori-related gastroenterology studies
- Infective Endocarditis Diagnosis and Management
- Fungal Plant Pathogen Control
- RNA and protein synthesis mechanisms
- Clostridium difficile and Clostridium perfringens research
- Fungal Biology and Applications
- Enterobacteriaceae and Cronobacter Research
- Infection Control in Healthcare
Trinity College Dublin
2014-2025
Dublin Dental University Hospital
2013-2025
University of Chicago
2010
Dublin City University
2009
Institut Pasteur
2009
Natural Environment Research Council
2009
Royal Prince Alfred Hospital
2005
University of Ulsan
2004
Le Moyne College
1996
Newcastle University
1994-1995
Summary: Atypical oral Candida isolates were recovered from 60 HIV-infected and three HIV-negative individuals. These organisms germ-tube-positive produced abundant chlamydospores which frequently arranged in triplets or contiguous pairs. They belonged to C. albicans serotype A had atypical carbohydrate assimilation profiles. Fingerprinting the genomic DNA of a selection these with albicans-specific probe 27A five separate oligonucleotides, homologous eukaryotic microsatellite repeat...
Candida dubliniensis is a recently described species of associated with oral candidiasis in human immunodeficiency virus (HIV)-infected individuals. Nineteen isolates C. recovered from 10 HIV-positive and 4 HIV-negative individuals one vaginal isolate an additional subject were assessed for fluconazole susceptibility by broth microdilution (BMD), hyphal elongation assessment, Etest. The susceptibilities these 20 to itraconazole amphotericin B ketoconazole also determined BMD only. Sixteen...
ABSTRACT Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with albicans , including the ability to form germ tubes and chlamydospores. These similarities have caused significant problems in identification of C. by average clinical mycology laboratory. To facilitate differentiation these species, we investigated growth 120 isolates 98 at 42 45°C on Emmons’ modified Sabouraud glucose agar (SGA) 10 each yeast-peptone-dextrose broth. None grew...
Candida dubliniensis is the closest known relative of albicans , most pathogenic yeast species in humans. However, despite both sharing many phenotypic characteristics, including ability to form true hyphae, C. a significantly less virulent and versatile pathogen. Therefore, identify -specific genes that may be responsible for an increased capacity cause disease, we have sequenced genome compared it with sequence. Although two sequences are highly similar synteny conserved throughout, 168...
Candida dubliniensis is a recently identified species which implicated in oral candidosis HIV-infected and AIDS patients. The shares many phenotypic characteristics with, phylogenetically closely related to, albicans . In this study the phylogenetic relationship between these two was investigated comparison of putative virulence factors performed. Four isolates C. from different clinical sources were chosen for with reference strains. First, distinct position further established by sequence...
ABSTRACT Candida dubliniensis is a recently described species associated with oral candidosis in human immunodeficiency virus (HIV)-infected and AIDS patients, from whom fluconazole-resistant clinical isolates have been previously recovered. Furthermore, derivatives exhibiting stable phenotype readily generated vitro fluconazole-susceptible following exposure to the drug. In this study, accumulated up 80% less [ 3 H]fluconazole than susceptible also exhibited reduced susceptibility metabolic...
Candida dubliniensis is a recently identified chlamydospore-positive yeast species associated with oral candidiasis in human immunodeficiency virus (HIV)-infected (HIV+) patients and closely related to albicans. Several recent reports have described atypical isolates phenotypic genetic properties similar those of C. dubliniensis. In this study 10 from HIV+ Switzerland, the United Kingdom, Argentina 1 isolate an HIV-negative Irish subject were compared reference strains albicans stellatoidea...
Candida albicans and dubliniensis are highly related pathogenic yeast species. However, C. is far more prevalent in human infection has been shown to be a wide range of models. Comparison the genomes two species revealed that they very similar although there some significant differences, largely due expansion virulence-related gene families (e.g., ALS SAP) albicans, increased levels pseudogenisation dubliniensis. Comparative global expression analyses have also used investigate differences...
A new group of serotype F bacteriophages Staphylococcus aureus has been found which mediates the simultaneous triple-lysogenic conversion enterotoxin A, staphylokinase and β-lysin. The phages were recovered from methicillin-resistant strains S. isolated in Irish hospitals between 1971 1988 strain PS42-D, used as propagating for typing phage 42D since before 1965. molecular mechanism triple mediated by three these was determined cloning, restriction endonuclease site mapping hybridization...
The phylogenetic position of Candida dubliniensis has previously been established on the basis sequence rRNA genes. In order to confirm relationship between C. and other yeast species, particularly albicans, using non-rRNA gene sequences ACT1 was chosen for analysis. Three overlapping fragments that together span entire (CdACT1) were amplified from a recombinant phage isolated genomic DNA λ library PCR. These cloned used determine contiguous gene. Analysis data revealed presence 1131 bp ORF...
ABSTRACT Candida dubliniensis is a newly recognized fungal pathogen causing mucosal disease in AIDS patients. Although preliminary studies indicate that most strains of C. are susceptible to established antifungal agents, fluconazole-resistant have been detected. Furthermore, easily derived vitro, and these exhibit increased expression multidrug resistance transporters, especially MDR1. Because the potential for development resistant , it prudent explore vitro activities several newer...
Summary To examine the molecular population genetics of M protein family Streptococcus pyogenes (group A Streptococcus) , 5′ regions polymerase chain reaction‐amplified emm products from 79 serotypes were sequenced and phylogeny was compared to estimates overall genetic relationships among strains determined by multilocus enzyme electrophoresis. Although sequences several designated as distinct types identical or almost identical, pattern is characterized very extensive variation. The...
Oligonucleotide fingerprinting of genomic DNA from oral isolates four different Candida species other than C. albicans and atypical chlamydospore-positive human immunodeficiency virus (HIV)-positive individuals AIDS patients was investigated as a means for differentiating between within individual species. Oligonucleotides composed simple repetitive sequence motifs, including (GACA)4, (GATA)4, (GGAT)4, (GTG)5, (GT)8, all yielded fingerprints suitable strain segregation 8 tropicalis isolates,...
The arginine catabolic mobile element (ACME) is prevalent among methicillin-resistant Staphylococcus aureus (MRSA) isolates of sequence type 8 (ST8) and staphylococcal chromosomal cassette mec (SCCmec) IVa (USA300) (ST8-MRSA-IVa isolates), evidence suggests that ACME enhances the ability ST8-MRSA-IVa to grow survive on its host. has been identified in a small number belonging other MRSA clones but widespread coagulase-negative staphylococci (CoNS). This study reports first description two...
The extension of germ tubes into elongated hyphae by Candida albicans is essential for damage host cells. C. albicans-specific gene EED1 plays a crucial role in this and maintenance filamentous growth. eed1Δ cells failed to extend long filaments switched back yeast growth after 3 h incubation during on plastic surfaces. Expression regulated the transcription factor Efg1 ectopic overexpression restored filamentation efg1Δ. Transcriptional profiling infection oral tissue revealed...
ABSTRACT Candida dubliniensis is a newly described species that closely related phylogenetically to albicans and commonly associated with oral candidiasis in human immunodeficiency virus-positive patients. Several recent studies have attempted elucidate phenotypic genotypic characteristics of use separating the two species. However, results obtained simple tests were too variable provided more definitive data complex for routine clinical laboratory setting. The objective this study was...