A5036396367- Protease and Inhibitor Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Body Contouring and Surgery
- Blood Coagulation and Thrombosis Mechanisms
- Biochemical and Structural Characterization
- Biological Stains and Phytochemicals
- Enzyme Production and Characterization
- Dermatological and COVID-19 studies
- Mast cells and histamine
- Cell death mechanisms and regulation
- Histone Deacetylase Inhibitors Research
- Contact Dermatitis and Allergies
- Vitamin C and Antioxidants Research
- Pesticide Exposure and Toxicity
- Facial Rejuvenation and Surgery Techniques
- Insect Resistance and Genetics
- Platelet Disorders and Treatments
- Skin and Cellular Biology Research
- Proteoglycans and glycosaminoglycans research
- Parvovirus B19 Infection Studies
- Chemical synthesis and alkaloids
- Calpain Protease Function and Regulation
- Dermatology and Skin Diseases
- Neuropeptides and Animal Physiology
- Plant-Derived Bioactive Compounds
Centro Universitário São Camilo
2021-2024
Hospital Sírio-Libanês
2020-2023
Universidade Federal de São Paulo
2004-2021
The interplay of different proteases and glycosaminoglycans is able to modulate the activity enzymes affect their structures. Human plasma kallikrein (huPK) a proteolytic enzyme involved in intrinsic blood clotting, kallikrein-kinin system fibrinolysis. We investigated effect heparin on action, inhibition secondary structure huPK. catalytic efficiency for hydrolysis substrates by huPK was determined Michaelis-Menten kinetic plots: 5.12×104 M-1 s-1 acetyl-Phe-Arg-p-nitroanilide, 1.40×105...
Caesalpinia echinata is a tree belonging to the Leguminosae family. The red color of trunk, looking like burning wood ('brasa' in Portuguese), origin name Brazil. Seeds leguminous plants contain high amounts serine proteinase inhibitors that can affect different biological processes. Here we show protein isolated from seeds C. able inhibit enzymes participate blood coagulation and fibrinolysis. This inhibitor (CeKI) was purified homogeneity by ion exchange reversed-phase chromatography....
Several proteins have been isolated from seeds of leguminous, but this is the first report that a protease was obtained Caesalpinia echinata Lam., tree belonging to Fabaceae family. This enzyme purified homogeneity by hydrophobic interaction and anion exchange chromatographies gel filtration. 61-kDa serine (CeSP) hydrolyses H-D-prolyl-L-phenylalanyl-L-arginine- p -nitroanilide (<mml:math...
In the plasma kallikrein-kinin system, it has been shown that when prekallikrein (PK) and high molecular weight kininogen (HK) assemble on endothelial cells, kallikrein (huPK) becomes available to cleave HK, releasing bradykinin, a potent mediator of inflammatory response. Because formation soluble glycosaminoglycans occurs concomitantly during processes, effect these polysaccharides interaction HK cell surface or extracellular matrix (ECM) two lines (ECV304 RAEC) was investigated. presence...
Inflammation is an essential process in many pulmonary diseases which kinins are generated by protease action on kininogen, a phenomenon that blocked inhibitors. We evaluated kinin release vivo lung inflammation model rats, the presence or absence of CeKI ( C. echinata kallikrein inhibitor), plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, rCeEI (recombinant elastase inhibits these proteases also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative...
Human neutrophil elastase (HNE) has been shown to be involved on death of different cell types, including epithelial lung cells, which is related several pulmonary diseases. Since HNE activity may influenced by extracellular matrix (ECM) molecules such as glycosaminoglycans (GAGs), and fibroblasts are the most common ECM-producing cells connective tissue, aim this work was verify if can induce fibroblast study enzyme modulation GAGs. HNE-like mimicked using human neutrophils conditioned...