A5040151622- Cancer, Hypoxia, and Metabolism
- Lung Cancer Treatments and Mutations
- Radiopharmaceutical Chemistry and Applications
- S100 Proteins and Annexins
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Angiogenesis and VEGF in Cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Bone health and treatments
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Renal cell carcinoma treatment
- Biomarkers in Disease Mechanisms
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Metabolism, Diabetes, and Cancer
- Kruppel-like factors research
- Lung Cancer Research Studies
- Gut microbiota and health
- Cancer Cells and Metastasis
- Lung Cancer Diagnosis and Treatment
- ATP Synthase and ATPases Research
- Chronic Lymphocytic Leukemia Research
Istituto Oncologico Veneto
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
Molecular Oncology (United States)
2021
Veneto Institute of Molecular Medicine
2017
University of Brescia
2011
University of Padua
1990-2011
Institut Català d'Oncologia
2011
University Medical Center of the Johannes Gutenberg University Mainz
2011
Johannes Gutenberg University Mainz
2011
MultiMedica
2011
// Anna Pastò 1,* , Chiara Bellio Giorgia Pilotto Vincenzo Ciminale 1,2 Micol Silic-Benussi 2 Giulia Guzzo 3 Andrea Rasola Frasson 4 Nardo Elisabetta Zulato Maria Ornella Nicoletto Mariangela Manicone Stefano Indraccolo 2,* and Alberto Amadori 1,2,* 1 Department of Surgery, Oncology, Gastroenterology, Oncology Section, University Padova, Italy Istituto Oncologico Veneto-IRCCS (IOV), Biomedical Sciences, Woman Child Health, Laboratory Hemato-Oncology, * These Authors contributed...
Cholangiocarcinoma (CCA) is characterized by an abundant stromal reaction. Cancer-associated fibroblasts (CAFs) are pivotal in tumor growth and invasiveness represent a potential therapeutic target. To understand the mechanisms leading to CAF recruitment CCA, we studied (1) expression of epithelial-mesenchymal transition (EMT) surgical CCA specimens cells, (2) lineage tracking enhanced green fluorescent protein (EGFP)-expressing human male cell line (EGI-1) after xenotransplantation into...
Cholangiocarcinoma (CCA) carries a severe prognosis because of its strong invasiveness and early metastasization. In several patients, otherwise eligible for surgical resection, micrometastasis are already present at the time surgery. The mechanisms responsible CCA unclear. S100A4, member S100 family small Ca(2+)-binding proteins, is expressed in mesenchymal cells, regulates cell motility types, some epithelial cancers. Thus, we aimed to study role S100A4 expression was studied by...
Abstract VEGF antagonists are now widely used cancer therapeutics, but predictive biomarkers of response or toxicity remain unavailable. In this study, we analyzed the effects anti-VEGF therapy on tumor metabolism and therapeutic by using an integrated set imaging techniques, including bioluminescence metabolic imaging, 18-fluorodeoxyglucose positron emission tomography, MRI spectroscopy. Our results revealed that caused a dramatic depletion glucose exhaustion ATP levels in tumors, although...
Anti-VEGF therapy perturbs tumor metabolism, severely impairing oxygen, glucose, and ATP levels. In this study, we investigated the effects of anti-VEGF in multiple experimental models that differ their glycolytic phenotypes to gain insights into optimal modulation metabolic features therapy. Prolonged treatments induced vascular regression necrosis xenograft models, with highly tumors becoming treatment resistant more rapidly than poorly tumors. By PET imaging, prolonged yielded an increase...
Abstract Purpose: LKB1 is a key sensor of metabolic stress, including hypoxia and glucose deprivation, two features the tumor microenvironment exacerbated by antiangiogenic therapy. We investigated role as potential predictive marker sensitivity to bevacizumab in advanced non–small cell lung cancer (aNSCLC). Experimental design: retrospectively analyzed expression IHC 98 samples from 125 patients with aNSCLC, 59 treated chemotherapy 39 plus bevacizumab. intensity was recoded classes...
Nuclear expression of the calcium-binding protein S100A4 is a biomarker increased invasiveness in cholangiocarcinoma, primary liver cancer with scarce treatment opportunities and dismal prognosis. In this study, we provide evidence that targeting nuclear import by low-dose paclitaxel, microtubule-stabilizing agent, inhibits cholangiocarcinoma metastatic spread. Administration paclitaxel to established (EGI-1) (CCA-TV3) cell lines expressing triggered marked reduction without modifying its...
Characterization of tumor-related genetic alterations is promising for the screening new predictive markers in non-small cell lung cancer (NSCLC). Aim study was to evaluate prognostic and role most frequent tumor-associated detected plasma before starting immune checkpoint inhibitors (ICIs).Between January 2017 October 2019, advanced NSCLC patients were prospectively screened with next-generation sequencing (NGS) while included two trials: VISION (NCT02864992), using Guardant360® test, MAGIC...
We investigated whether Circulating Tumor Cells (CTCs) isolated from epithelial tumors could survive and grow in xenotransplants.To this purpose, EpCAM-positive CTCs were enriched by CellSearch platform the only FDA-cleared automated that quantifies tumor burden peripheral blood provides clinical evidence of predictive prognostic value.The metastatic prostate (n=6) breast (n=2) cancer patients.The xenograft assay was developed 8-week-old NOD/SCID mice subcutaneously injected with increasing...
Abstract Fibroblast growth factor-8b (FGF8b) exerts nonredundant autocrine/paracrine functions in steroid hormone–regulated tumors. Previous observations had shown that the soluble pattern recognition receptor long pentraxin-3 (PTX3) is a natural selective antagonist for restricted number of FGF family members, inhibiting FGF2 but not FGF1 and FGF4 activity. Here, we assessed capacity PTX3 to antagonize FGF8b inhibit vascularization Surface plasmon resonance analysis shows binds with high...
Liquid biopsy has the potential to monitor biological effects of treatment. KRAS represents most commonly mutated oncogene in Caucasian non-small-cell lung cancer (NSCLC). The aim this study was explore association dynamic plasma genotyping with outcome advanced NSCLC patients.Advanced patients were prospectively enrolled. Plasma samples collected at baseline (T1), after 3 or 4 weeks, according treatment schedule (T2) and first radiological restaging (T3). Patients carrying mutation tissue...
Abstract Background Immune checkpoint inhibitors (ICIs) have revolutionised treatment of advanced non-small cell lung cancer (aNSCLC), but a proportion patients had no clinical benefit and even experienced detrimental effects. This study aims to characterise experiencing hyperprogression (HPD) early death (ED) by longitudinal liquid biopsy. Methods aNSCLC receiving ICIs were prospectively enrolled. Plasma was collected at baseline (T1) after 3/4 weeks treatment, according the schedule (T2)....
ctDNA is a useful tool for NGS molecular profiling in advanced NSCLC patients. Its clinical applicability patients with gene rearrangements still limited due to lower detection rate of these types alterations compared single SNVs or small indels. To this purpose, we performed study two Italian centers assess the concordance between tissue and plasma samples genes fusions (ALK, ROS, RET) METexon14 mutations Patients histological diagnosis oncogene addicted ROS1, RET positive mutated) were...
Molecular profiling of advanced EGFR mutated NSCLC has recently demonstrated the co-existence multiple genetic alterations. Specifically, co-existing KRAS-mutations in NSCLCs have been described, despite their prevalence at progression and role response to tyrosine kinase inhibitors (TKIs) remain marginally explored. Aim our study was investigate KRAS mutations time progressive disease explore impact on clinical outcome.We retrospectively analyzed by digital droplet PCR co-mutations 106...
Metabolic profiling of cancer is a rising interest in the field biomarker development. One bottleneck its clinical exploitation, however, lack simple and quantitative techniques that enable to capture key metabolic traits tumor from archival samples. In fact, liquid chromatography associated with mass spectrometry gold-standard technique for study metabolism because it has high levels accuracy precision. However, requires freshly frozen samples, which are difficult collect large...
Genetic screening of somatic mutations in circulating free DNA (cfDNA) opens up new opportunities for personalized medicine. In this study, we aim to illustrate the implementation NGS-based liquid biopsy clinical practice detection alterations selected genes. Our work is particularly relevant diagnosis and treatment NSCLC. Beginning 2020, implemented use Roche’s Avenio ctDNA expanded panel our diagnostic routine. retrospectively review genetic tests performed laboratory, focusing on key...