A5091139188- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Enzyme Catalysis and Immobilization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
- Microbial Metabolic Engineering and Bioproduction
- Hydrogels: synthesis, properties, applications
- Nanofabrication and Lithography Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Polymer Surface Interaction Studies
- Pickering emulsions and particle stabilization
- Acoustic Wave Resonator Technologies
- biodegradable polymer synthesis and properties
- Analytical Chemistry and Sensors
- Monoclonal and Polyclonal Antibodies Research
- Electrohydrodynamics and Fluid Dynamics
- Advanced Materials and Mechanics
Karlsruhe Institute of Technology
2015-2023
Max Planck Institute of Colloids and Interfaces
2022
Biolog Life Science Institute
2022
FIZ Karlsruhe – Leibniz Institute for Information Infrastructure
2018
The development of process steps catalyzed by immobilized enzymes usually encompasses the screening enzyme variants, as well optimization immobilization protocols and parameters. Direct biocatalysts physical entrapment into hydrogels can be applied to reduce effort required for immobilization, enzyme-specific procedure is omitted. Physical applicable purified crude cell extracts. Therefore, it used quickly assess compare activities enzymes. For application in flow reactors, we developed...
Abstract The outcome of three-dimensional (3D) bioprinting heavily depends, amongst others, on the interaction between developed bioink, printing process, and equipment. However, if this interplay is ensured, promises unmatched possibilities in health care area. To pave way for comparing newly biomaterials, clinical studies, medical applications (i.e. printed organs, patient-specific tissues), there a great need standardization manufacturing methods order to enable technology transfers....
Physical entrapment of enzymes within a porous matrix is fast and gentle process to immobilize biocatalysts enable their recycling long-term use. This study introduces the development biocompatible 3D-printing material suitable for enzyme entrapment, while having good rheological UV-hardening properties. Three different viscosity-enhancing additives have been tested in combination with poly(ethylene glycol) diacrylate-based hydrogel system. The addition polyxanthan or hectorite clay...
In biotechnology, immobilization of functional reactants is often done as a surface on small particles. Examples are chromatography columns and fixed-bed reactors. However, the available for directly linked to particle diameter bed porosity these systems, leading high backpressure sizes. When larger molecules, such enzymes immobilized, physical entrapment within porous materials like hydrogels an alternative. An emerging technique production geometrically structured, three-dimensional...
We propose surface acoustic wave (SAW) resonators as a complementary tool for conditioning film monitoring. Conditioning films are formed by adsorption of inorganic and organic substances on substrate the moment this comes into contact with liquid phase. In case implant insertion, instance, initial protein is required to start wound healing, but it will also trigger immune reactions leading inflammatory responses. The control would allow promote healing process suppress adverse reactions....
Bioprinting is gaining importance for the manufacturing of tailor-made hydrogel scaffolds in tissue engineering, pharmaceutical research and cell therapy. However, structure fidelity geometric deviations printed objects heavily influence mass transport process reproducibility. Fast, three-dimensional nondestructive quality control methods will be decisive approval larger studies or industry. Magnetic resonance imaging (MRI) meets these requirements characterizing heterogeneous soft materials...
Abstract The immobilization of enzymes into polymer hydrogels is a versatile approach to improve their stability and utility in biotechnological biomedical applications. However, these systems typically show limited enzyme activity, due unfavorable pore dimensions low accessibility. Here, 3D jet writing water‐based bioinks, which contain preloaded enzymes, used prepare hydrogel scaffolds with well‐defined, tessellated micropores. After writing, the are chemically modified via...
Bioprinting is a method to fabricate 3D models that mimic tissue. Future fields of application might be in pharmaceutical or medical context. As the number applicants vary between only one patient manufacturing tissue for high-throughput drug screening, designing process will necessitate high degree flexibility, robustness, as well comprehensive monitoring. To enable quality by design optimisation future application, establishing systematic data storage routines suitable automated analytical...
As virtual reality (VR) has drastically evolved over the past few years, field of applications VR flourished way beyond gaming industry. While commercial solutions might be available, there is a need to develop workflow for specific applications. Bioprinting represents such an example. Here, complex 3D data generated and needs visualized in context quality control. We demonstrate that transfer commercially available software possible by introducing optimized workflow. In present work, we...
A time-dependent understanding of swelling characteristics and external stimuli behavior is crucial for the development functional hydrogels. Magnetic resonance imaging (MRI) offers opportunity to study three-dimensional (3D) soft materials nondestructively. This technique already widely used as an image-based medical diagnostic tool applied here evaluate complex structures a hydrogel—a double network chemically crosslinked casein enhanced with alginate—fabricated by 3D printing. When...
integriertes Verfahren unter Verwendung einer PDMS-Mikroform aus cPEVA und Dicumylperoxid als temperatursensitiver Vernetzer hergestellt