A5033541350- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Intraocular Surgery and Lenses
- Peripheral Nerve Disorders
- Optical Coherence Tomography Applications
- Surface Roughness and Optical Measurements
- Optical measurement and interference techniques
- Image Processing and 3D Reconstruction
- Advanced Fluorescence Microscopy Techniques
- Advanced Measurement and Metrology Techniques
- Orthopedic Surgery and Rehabilitation
- Non-Invasive Vital Sign Monitoring
- Elbow and Forearm Trauma Treatment
- Advancements in Transdermal Drug Delivery
- Tendon Structure and Treatment
- Ophthalmology and Eye Disorders
- Bioactive Compounds and Antitumor Agents
- Nerve Injury and Rehabilitation
- Corneal Surgery and Treatments
- Optical Imaging and Spectroscopy Techniques
- Retinal and Macular Surgery
- Spatial Cognition and Navigation
- Neurology and Historical Studies
- Retinal Development and Disorders
Karlsruhe Institute of Technology
2014-2023
Institut für Assistenzsysteme und Qualifizierung
2020
University of Alberta
2003-2019
University of Rostock
2012-2018
Jena University Hospital
2016
Universitätsmedizin Greifswald
2016
Applied Materials (Germany)
2015
Triemli Hospital
2014
ZHAW Zurich University of Applied Sciences
2010-2014
Institut Für Angewandte Kulturforschung
2011
The QRS complex is the most striking waveform within electrocardiogram (ECG). Since it reflects electrical activity heart during ventricular contraction, time of its occurrence as well shape provide much information about current state heart. Due to characteristic serves basis for automated determination rate, an entry point classification schemes cardiac cycle, and often also used in ECG data compression algorithms. In that sense, detection provides fundamentals almost all analysis Software...
We sought to determine whether early nerve damage may be detected by corneal confocal microscopy (CCM), skin biopsy, and neurophysiological tests in 86 recently diagnosed type 2 diabetic patients compared with 48 control subjects. CCM analysis using novel algorithms reconstruct fiber images was performed for all fibers major (MNF) only. Intraepidermal density (IENFD) assessed specimens. Neurophysiological measures included conduction studies (NCS), quantitative sensory testing (QST),...
Bidimensional regression is a method for comparing the degree of resemblance between 2 planar configurations points and, more generally, assessing nature geometry (Euclidean and non-Euclidean) 2-dimensional independent dependent variables. For example, it can assess similarity location estimates from different tasks or participant groups, measure fidelity cognitive maps actual locations, provide parameters psychological process models. The authors detail formal uni- bidimensional regression,...
Optical coherence tomography (OCT) of the retina and corneal confocal laser scanning microscopy (CLSM) subbasal nerve plexus (SBP) are noninvasive techniques for quantification ocular neurodegenerative changes in individuals with type 1 diabetes mellitus (T1DM). In adult T1DM patients these hardly related to only. Instead, ageing and/or lifestyle associated comorbidities have be considered as putative confounding variables. Therefore, we investigated pediatric (n = 28; 14.2 ± 2.51 y;...
Purpose.: A growing number of studies provide evidence that the morphology corneal subbasal nerve plexus (SNP), examined by confocal microscopy (CCM), is a sensitive marker for diabetic peripheral neuropathy. However, it has been established field view single CCM image (≈0.16 mm2) insufficient reliable assessment fiber morphology. The present work proposes highly automated technique imaging an extended area SNP and creating large-scale montages. Methods.: moving fixation target presented on...
We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 396 µm3. The microscope objective used is equipped piezo actuator automated, fast and precise closed-loop focal plane control. Furthermore, we novel concave surface contact cap, which significantly reduces eye movements by 87%, hence increasing overlapping image area whole stack. This increases cuboid generated...
Purpose: To determine if corneal subbasal nerve plexus (SBP) parameters derived from wide-area depth-corrected mosaic images are associated with type 2 diabetes. Methods: One hundred sixty-three mosaics were produced eyes of 82 subjects by laser-scanning in vivo confocal microscopy (IVCM). Subjects the same age, without (43 subjects) or diabetes (39 subjects). Mosaic fiber length density (mCNFL) and apical whorl (wCNFL) quantified related to presence duration (short < 10 years long ≥ years)....
Abstract The capability of corneal confocal microscopy (CCM) to acquire high-resolution in vivo images the densely innervated human cornea has gained considerable interest using this non-invasive technique as an objective diagnostic tool for staging peripheral neuropathies. Morphological alterations subbasal nerve plexus (SNP) assessed by CCM have been shown correlate well with progression neuropathic diseases and even predict future-incident neuropathy. Since field view single is...
Purpose: The corneal subbasal nerve plexus (SNP) offers high potential for early diagnosis of diabetic peripheral neuropathy. Changes in fibers can be assessed vivo by confocal laser scanning microscopy (CLSM) and quantified using specific parameters. While current study results agree regarding parameter tendency, there are considerable differences terms absolute values. present set out to identify factors that might account this variability.
Abstract The outcome of three-dimensional (3D) bioprinting heavily depends, amongst others, on the interaction between developed bioink, printing process, and equipment. However, if this interplay is ensured, promises unmatched possibilities in health care area. To pave way for comparing newly biomaterials, clinical studies, medical applications (i.e. printed organs, patient-specific tissues), there a great need standardization manufacturing methods order to enable technology transfers....
Abstract A dense nerve plexus in the clear outer window of eye, cornea, can be imaged vivo to enable non-invasive monitoring peripheral degeneration diabetes. However, a limited field view corneal nerves, operator-dependent image quality, and subjective sampling methods have led difficulty establishing robust diagnostic measures relating progression diabetes its complications. Here, we use machine-based algorithms provide wide-area mosaics cornea’s subbasal (SBP) also accounting for depth...
Printed electronics offer great potential for new applications such as Internet of Things devices and wearables. However, to date, only a limited number electronic functions integration densities can be realised by printing processes. Hence, hybrid printed circuits are actually created mounting silicon components. Since both materials processes continuously evolving, an accompanying structured development methodology is required. This paper highlights digital workflow from design automated...
Glyfoline (4, 1,6-dihydroxy-10-methyl-2,3,4,5-tetramethoxyacridin-9-one) and its congeners were synthesized for evaluation of their cytotoxicity. A detailed structure-activity relationships (SAR) these acridone derivatives also studied. To study the SAR glyfoline analogues, substituent(s) at C-1 C-6 heterocyclic nitrogen nucleus modified. Nitro- amino-substituted analogues to effects (electron-withdrawing vs electron-donating) on These compounds via Ullmann condensation anthranilic acids...
Abstract Small fiber neuropathy (SFN) has been suggested as a trigger of restless legs syndrome (RLS). An increased prevalence peripheral demonstrated in Parkinson’s disease (PD). We aimed to investigate, cross-sectional manner, whether SFN is overrepresented PD patients with concurrent RLS relative without RLS, using vivo corneal confocal microscopy (IVCCM) and quantitative sensory testing (QST) part small assessment. Study participants comprised age- sex-matched ( n = 21) 21), controls...
During breast cancer therapy, paclitaxel and trastuzumab are both associated with adverse effects such as chemotherapy-induced peripheral neuropathy other systemic side including ocular complications. Corneal nerves considered part of the nervous system can be imaged non-invasively by confocal laser scanning microscopy (CLSM) on cellular level. Thus, in vivo CLSM imaging structures corneal subbasal nerve plexus (SNP) sensory or dendritic cells (DCs) a powerful tool for assessment...
Regarding the growing interest and importance of understanding cellular changes cornea in diseases, a quantitative characterization epithelium is becoming increasingly important. Towards this, latest research offers considerable improvements imaging by confocal laser scanning microscopy (CLSM). This study presents pipeline to generate normative morphological data epithelial cell layers healthy human corneas.3D vivo CLSM was performed on eyes volunteers (n=25) with Heidelberg Retina Tomograph...
The morphometric assessment of the corneal subbasal nerve plexus (SNP) by confocal microscopy holds great potential as a sensitive biomarker for various ocular and systemic conditions diseases. Automated wide-field montages (or large-area mosaic images) SNP provide an opportunity to overcome limited field view available imaging systems without need manual, subjective image selection characterization. However, current montaging solutions usually calculate after examination session, reliable...
Purpose/Aim of the study: A recently proposed technique enables generation continuously increasing mosaic images corneal sub-basal nerve plexus (SNP) using in vivo confocal microscopy (CCM). The aim present study was to investigate progression fiber length (CNFL) measured growing with regard their area.Materials and Methods: Five large datasets from three healthy volunteers were examined CCM technique. Intermediate created assessed for CNFL.Results: CNFL shows both over- underestimation...
In vivo confocal microscopy (IVCM) is a non-invasive imaging technique facilitating real-time acquisition of images from the live cornea and its layers with high resolution (1-2 µm) magnification (600 to 800-fold). IVCM extensively used examine at cellular level, including subbasal nerve plexus (SBNP). has thus gained intense interest for probing ophthalmic systemic diseases affecting peripheral nerves. One main drawbacks, however, small field view IVCM, preventing an overview SBNP...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of cyclopentanthraquinones: analogs mitomycin CBernd Kohler, Tsann Long Su, Ting Chao Chou, Xiang Jun Jiang, and Kyoichi A. WatanabeCite this: J. Org. Chem. 1993, 58, 7, 1680–1686Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/jo00059a014https://doi.org/10.1021/jo00059a014research-articleACS PublicationsRequest reuse permissionsArticle...
Corneal confocal microscopy (CCM) has revealed reduced corneal nerve fiber (CNF) length and density (CNFL, CNFD) in patients with diabetes, but the spatial pattern of CNF loss not been studied. We aimed to determine whether analysis distribution branching points (CNBPs) may contribute improving detection early loss. hypothesized that decline follows a clustered rather than random CNBPs. CCM, conduction studies (NCS), quantitative sensory testing (QST) were performed cross-sectional study...
Confocal in vivo microscopy is an established method ophthalmology research. As it requires contact coupling and calibration of the instruments suboptimal, this has been only rarely used clinical routine work. a result close collaboration between physicists, information scientists ophthalmologists, confocal laser scanning (CLSM) eye developed recent years prototype can now be patients. The present study evaluates possible uses method.The essential innovations CLSM are (1) newly designed...
Introduction . Diabetic neuroosteoarthropathy (DNOAP) early symptoms are unspecific, mimicking general infectious and rendering a diagnosis challenging. Consequently, unfavourable outcomes occur frequently, with recurrent foot ulceration, complications, eventually amputation. Corneal confocal microscopy (CCM) of the subbasal nerve plexus (SNP) is used to detect peripheral neuropathy in diabetic patients without retinopathy. This pilot study was designed determine if specific SNP changes...