A5068420554- Axon Guidance and Neuronal Signaling
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Angiogenesis and VEGF in Cancer
- Immunodeficiency and Autoimmune Disorders
- Allergic Rhinitis and Sensitization
- Animal Behavior and Reproduction
- Plant-based Medicinal Research
- Bird parasitology and diseases
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Primate Behavior and Ecology
- Contact Dermatitis and Allergies
- Pediatric health and respiratory diseases
- Apelin-related biomedical research
- Blood disorders and treatments
- Vector-borne infectious diseases
- Chemokine receptors and signaling
- Mycobacterium research and diagnosis
McGill University Health Centre
2016-2017
McGill University
2011-2016
Christie (Canada)
2011-2015
King Saud University
2011
Harvard University
1981
Abstract The increase in airway smooth muscle (ASM) mass is a major structural change asthma. This has been attributed to ASM cell (ASMC) hyperplasia and hypertrophy. distance between ASMC the epithelium reduced, suggesting migration of cells toward epithelium. Recent studies have suggested role chemokines epithelium; however, other biological effects. objective current study test hypothesis that (eotaxin, RANTES, IL-8, MIP-1α) can directly influence by increasing rate proliferation or...
The regulatory properties of B cells have been studied in autoimmune diseases; however, their role allergic diseases is poorly understood. We demonstrate that Semaphorin 4C (Sema4C), an axonal guidance molecule, plays a crucial cell function. Mice deficient Sema4C exhibited increased airway inflammation after allergen exposure, with massive eosinophilic lung infiltrates and Th2 cytokines. This phenotype was reproduced by mixed bone marrow chimeric mice only cells, indicating lymphocytes were...
Semaphorins are important molecules in embryonic development and multiple semaphorins have been identified as having key roles immune regulation. To date, there is little known about Semaphorin 4C (Sema4C) biology. We report for the first time that Sema4C inducible human murine B-cells may be normal B-cell development. Human tonsillar were studied following activation via anti-CD40 antibodies presence or absence of representative Th1, Th2, regulatory cytokines. Murine from WT Sema4C-/- mice...
Abstract IVIg is widely used as an immunomodulatory therapy. We have recently demonstrated that protects against airway hyperresponsiveness (AHR) and inflammation in mouse models of allergic airways disease (AAD), associated with induction Foxp3+ regulatory T cells (Treg). Using mice carrying a DTR/EGFP transgene under the control Foxp3 promoter (DEREG mice), we demonstrate this study generates de novo population peripheral Treg (pTreg) absence endogenous Treg. IVIg-generated pTreg were...
Methods Wild-type and Sema4C mice were sensitized intraperitoneally using 100 μL OVA (0.5 mg/mL ovalbumin 4 aluminum hydroxide in PBS) on days 0 14, challenged intranasally 20 (10 from 28 to 30. Sacrifice analysis of Airway Hyperresponsiveness via flexiVent was performed day 31. Serum IgE IL-10 expression levels measured by ELISA. B cells phenotyped fluorescence-activated cell sorting (FACS). motility migration assays.
Abstract We have shown that induced regulatory T cells (iTreg) are necessary and sufficient to rescue antigen-challenged mice in a murine model of allergic airways disease (AAD). Intravenous immunoglobulin (IVIg) treatment wild-type sensitized challenged antigen induces Treg dendritic-cell (DC) dependent mechanism. In the present study, DEREG (which co-express diphtheria toxin (DT) receptor with Foxp3, allowing for selective transient depletion Treg) were treated IVIg after all endogenous...
Background IVIg is a polyclonal IgG preparation with potent immune-modulating properties. We demonstrated that protects against airway hyperreactivity (AHR) and inflammation in mouse models of allergic disease, accompanied by peripheral induction Foxp3 regulatory T-cells (iTreg). The requirement IVIginduced iTreg their antigen-specificity attenuation AHR remains unknown.
Abstract We have previously found that mice with Semaphorin 4C (Sema4C) deficient B cells exhibited exaggerated airway damage and pulmonary inflammation during allergic airways disease (AAD). aimed to determine the mechanisms by which Sema4C on B-cells regulates AAD. Following allergen exposure, Sema4C-/- AAD had significantly fewer CD138high IL-10+ (CD138high Breg) increased IL4+ in spleen. In vitro, consistently failed differentiate into Breg under Th2 culture conditions. Adoptive transfer...
Abstract The dendritic cell immunoreceptor (DCIR) is an inhibitory C-type lection receptor possessing immunoregulatory properties. We have shown that DCIR serves as a for IVIg. In this study, we sought to determine its requirement in modulating IVIg mechanism of action by using murine model allergic airways disease. Wild type (WT) and DCIR−/− mice were sensitized challenged with ovalbumin (OVA), lung histopathology, bronchoalveolar lavage phenotyping inflammatory cells performed. further...
SUMMARY A relatively inexpensive microcomputer system was developed to store and manipulate clinical pathologic data from a large colony of nonhuman primates. The programming for the designed so that computer instructs users, who may have no knowledge programming, how enter all kinds command it answer any ad hoc queries about data. Minimum input professional programmer is required. As many as 4 investigators use simultaneously by means satellite terminals located in several areas throughout...