A5101694906- Axon Guidance and Neuronal Signaling
- Alzheimer's disease research and treatments
- Pneumonia and Respiratory Infections
- Microbial infections and disease research
- Angiogenesis and VEGF in Cancer
- Cholinesterase and Neurodegenerative Diseases
- Aquaculture disease management and microbiota
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
- Asthma and respiratory diseases
- Respiratory viral infections research
- Mycobacterium research and diagnosis
- Plant-based Medicinal Research
- Evaluation Methods in Various Fields
- Allergic Rhinitis and Sensitization
- Prion Diseases and Protein Misfolding
- Immunotherapy and Immune Responses
- Tryptophan and brain disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Pharmaceutical and Antibiotic Environmental Impacts
- Immune Cell Function and Interaction
- Polysaccharides and Plant Cell Walls
- Nuclear Receptors and Signaling
- Toxoplasma gondii Research Studies
Ningxia University
2011-2022
Christie (Canada)
2014-2017
McGill University
2013-2017
Ministry of Education of the People's Republic of China
2014-2017
McGill University Health Centre
2016-2017
Tsinghua University
2010-2012
Shanghai University
2011
Mycoplasma ovipneumoniae (M. ovipneumoniae) is a species of bacteria that specifically infects sheep and goat, causing ovine infectious pleuropneumonia. However, the mechanism underlying pathogen-host interaction between M. airway epithelial cells unknown. A primary air-liquid interface (ALI) culture model generated from bronchial Ningxia Tan (ovis aries) was employed to explore potential ovipneumoniae-induced cell apoptosis by characterizing production reactive oxygen (ROS), methane...
In an attempt to better understand the pathogen-host interaction between invading Mycoplasma ovipneumoniae (M. ovipneumoniae) and sheep airway epithelial cells, biological effects possible molecular mechanism of capsular polysaccharide M. (CPS) in induction cell apoptosis were explored using bronchial cells cultured air-liquid interface (ALI). The CPS was first isolated purified. Results showed that had a cytotoxic effect by disrupting integrity mitochondrial membrane, accompanied with...
Mycoplasma ovipneumoniae ( M. ) is characterized as an etiological agent of primary atypical pneumonia that specifically infects sheep and goat. In attempt to better understand the pathogen-host interaction between invading airway epithelial cells, we investigated host inflammatory responses against capsular polysaccharide (designated CPS) using bronchial cells cultured in air-liquid interface (ALI) model. Results showed CPS derived from could activate toll-like receptor- (TLR-) mediated...
The regulatory properties of B cells have been studied in autoimmune diseases; however, their role allergic diseases is poorly understood. We demonstrate that Semaphorin 4C (Sema4C), an axonal guidance molecule, plays a crucial cell function. Mice deficient Sema4C exhibited increased airway inflammation after allergen exposure, with massive eosinophilic lung infiltrates and Th2 cytokines. This phenotype was reproduced by mixed bone marrow chimeric mice only cells, indicating lymphocytes were...
Amyloid-β (Aβ40/42 ) aggregates containing the cross-β-sheet structure are associated with pathogenesis of Alzheimer's disease (AD). It is generally accepted that N-terminal peptide Aβ40/42 , Aβ1-16 does not aggregate, and cytotox
Semaphorins are important molecules in embryonic development and multiple semaphorins have been identified as having key roles immune regulation. To date, there is little known about Semaphorin 4C (Sema4C) biology. We report for the first time that Sema4C inducible human murine B-cells may be normal B-cell development. Human tonsillar were studied following activation via anti-CD40 antibodies presence or absence of representative Th1, Th2, regulatory cytokines. Murine from WT Sema4C-/- mice...
Summary The uncomposted faeces of dairy cow are usually stacked on breeding farms, dried under natural conditions and then used as bedding material or they may be continuously piled up. However, no information is available to evaluate variations in the human animal pathogen genes antibiotic resistance during accumulation fresh manure. Here, we present metagenomic analysis manure from a farm Ning Xia, showing unique enrichment (ARGs) We found that could significantly increase diversity...
Bovine tuberculosis is an airborne infectious disease caused by organisms of the Mycobacterium (MTB) complex. Mycolic acid (MA) main lipid component cell membrane MTB. It non-enzymatically reduced NAD(P)H and further produces reactive oxygen species (ROS), which can cause oxidative stress in human cells. N-acetylcysteine (NAC) a synthetic precursor glutathione (GSH) exhibits anti-ROS activity. However, underlying mechanisms its protective properties remain uncertain. Herein, after...
Abstract IVIg is widely used as an immunomodulatory therapy. We have recently demonstrated that protects against airway hyperresponsiveness (AHR) and inflammation in mouse models of allergic airways disease (AAD), associated with induction Foxp3+ regulatory T cells (Treg). Using mice carrying a DTR/EGFP transgene under the control Foxp3 promoter (DEREG mice), we demonstrate this study generates de novo population peripheral Treg (pTreg) absence endogenous Treg. IVIg-generated pTreg were...
Amyloid-β (Aβ) plays a pivotal role in Alzheimer's disease (AD) pathogenesis and toxic mechanisms such as oxidative stress, mitochondrial dysfunction, calcium turbulence, apoptosis induction. Therefore, interfering with Aβ aggregation has long
Methods Wild-type and Sema4C mice were sensitized intraperitoneally using 100 μL OVA (0.5 mg/mL ovalbumin 4 aluminum hydroxide in PBS) on days 0 14, challenged intranasally 20 (10 from 28 to 30. Sacrifice analysis of Airway Hyperresponsiveness via flexiVent was performed day 31. Serum IgE IL-10 expression levels measured by ELISA. B cells phenotyped fluorescence-activated cell sorting (FACS). motility migration assays.
Lycium barbarum berries have been utilized in Asia for many years. However, the mechanisms of its lung-defensive properties are indeterminate.We investigate whether L. polysaccharide (LBP) could weaken Pseudomonas aeruginosa infection-induced lung injury.Mice primary air-liquid interface epithelial cultures were pretreated with LBP and subsequently treated pyocyanin (PCN). Lung injury, including apoptosis, inflammation, oxidative stress, was estimated by western blot, enzyme-linked...
Abstract We have shown that induced regulatory T cells (iTreg) are necessary and sufficient to rescue antigen-challenged mice in a murine model of allergic airways disease (AAD). Intravenous immunoglobulin (IVIg) treatment wild-type sensitized challenged antigen induces Treg dendritic-cell (DC) dependent mechanism. In the present study, DEREG (which co-express diphtheria toxin (DT) receptor with Foxp3, allowing for selective transient depletion Treg) were treated IVIg after all endogenous...
Abstract We have previously found that mice with Semaphorin 4C (Sema4C) deficient B cells exhibited exaggerated airway damage and pulmonary inflammation during allergic airways disease (AAD). aimed to determine the mechanisms by which Sema4C on B-cells regulates AAD. Following allergen exposure, Sema4C-/- AAD had significantly fewer CD138high IL-10+ (CD138high Breg) increased IL4+ in spleen. In vitro, consistently failed differentiate into Breg under Th2 culture conditions. Adoptive transfer...