A5100420107- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Ocular Oncology and Treatments
- Circular RNAs in diseases
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Cancer-related Molecular Pathways
- Osteoarthritis Treatment and Mechanisms
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Wnt/β-catenin signaling in development and cancer
- vaccines and immunoinformatics approaches
- Intensive Care Unit Cognitive Disorders
- Neurological Disease Mechanisms and Treatments
Tianjin Medical University Cancer Institute and Hospital
2022-2025
Chinese Academy of Agricultural Sciences
2022-2025
Sichuan University
2023-2024
West China Hospital of Sichuan University
2023-2024
Shanghai Jiao Tong University
2014-2024
Shanghai East Hospital
2019-2024
Jinggangshan University
2023-2024
Guangdong Medical College
2021-2024
Tongji University
2020-2024
Kyoto University
2023-2024
Rationale: It is known that neuroinflammation plays a critical and detrimental role in the development of cerebral ischemia/reperfusion (I/R), but regulation cyclic GMP-AMP synthase (cGAS)-mediated innate immune response I/R-induced largely unexplored. This study aimed to investigate function regulatory mechanism cGAS brain injury, identify possible strategies for treatment ischemic stroke. Methods: To demonstrate microglial histone deacetylase 3 (HDAC3) regulates cGAS-stimulator interferon...
The Atlantic herring is a model species for exploring the genetic basis ecological adaptation, due to its huge population size and extremely low differentiation at selectively neutral loci. However, such studies have so far been hampered because of highly fragmented genome assembly. Here, we deliver chromosome-level assembly based on hybrid approach combining de novo Pacific Biosciences (PacBio) with Hi-C-supported scaffolding. comprises 26 autosomes sizes ranging from 12.4 33.1 Mb total...
Osteoarthritis (OA), a chronic degenerative disease with heterogeneous properties, is difficult to cure due its complex pathogenesis. Curcumin possesses excellent anti-inflammatory and antioxidant properties may have potential therapeutic value in OA. In this study, we investigated the action targets of curcumin identified anti-OA for curcumin. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) signaling pathway analyses were performed evaluate these targets. Furthermore, established...
Elderly patients are reportedly at higher risk of postoperative cognitive dysfunction (POCD) after inhalational anesthesia with sevoflurane. We hypothesized that the incidence POCD would be in elderly undergoing major surgery under rather than intravenous anesthesia. also measured plasma S-100β protein concentration as a biomarker central nervous system injury, and interleukin (IL)-6 tumor necrosis factor (TNF)-α concentrations to judge contribution systemic inflammation POCD. Ninety aged...
Kcnq1ot1 is a long noncoding ribonucleic acid (RNA; lncRNA) that participates in the regulation of genes within Kcnq1 imprinting domain. Using novel RNA-guided chromatin conformation capture method, we demonstrate 5′ region RNA orchestrates long-range intrachromosomal loop between KvDMR1 and promoter required for maintenance imprinting. PRC2 (polycomb repressive complex 2), which allelic repression Kcnq1, also recruited by via EZH2. Targeted suppression lncRNA prevents creation this causes...
Abstract Background Long non-coding RNAs (lncRNAs) are not translated into proteins and were initially considered to be part of the ‘dark matter’ genome. Recently, it has been shown that lncRNAs play a role in recruitment chromatin modifying complexes can influence gene expression. However, is unknown if function similar way cancer. Results Here, we show lncRNA ROR occupies activates TESC promoter by repelling histone G9A methyltransferase promoting release H3K9 methylation. Suppression...
The design of the mRNA vaccine involves selection in vitro transcription (IVT) systems and nonviral delivery vectors. This study aimed to verify effect 5' 3' untranslated region (UTR) sequences on translation efficiency mRNA. Three modes IVT-mRNA (IVT-mRNA-n1/n2/n3) with diverse UTRs were constructed, EGFP (enhanced green fluorescent protein) HA (hemagglutinin) gene H3N2 influenza virus introduced into each them. results showed that mode originating from human β-globulin was better than...
Long noncoding RNAs (lncRNAs) are proved to be critical regulators in numerous cellular processes. However, the potential involvement of lncRNAs macroautophagy/autophagy is largely unknown. Autophagy a highly regulated degradation system, and its dysregulation involved many human diseases, including cancers. Here, we show that lncRNA ZNNT1 induced by PP242 MTORC1 selective inhibitor rapamycin uveal melanoma (UM) cells. Overexpression promotes autophagy upregulating ATG12 expression, whereas...
Tumor exosomes with molecular marker-proteins inherited from their parent cells have emerged as a promising liquid biopsy biomarker for cancer diagnosis. However, facile, robust, and sensitive detection of exosomal proteins remains challenging. Therefore, nanozyme sensor array is constructed by using aptamer-modified C3N4 nanosheets (Apt/C3N4 NSs) together solvent-mediated signal amplification strategy ratiometric fluorescence proteins. Three aptamers specific to are selected construct...
Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. However, the treatment efficacy survival of iPSC-CMs vivo after transplantation yielded inconsistent results.The objective this study was to evaluate ability human improve function a rat postinfarction model.Eight-week-old male Sprague-Dawley rats were randomly selected receive an intramyocardial injection...
Abstract Background Long non-coding RNAs (lncRNAs) have been identified as important epigenetic regulators that play critical roles in human cancers. However, the regulatory functions of lncRNAs tumorigenesis remains to be elucidated. Here, we aimed investigate molecular mechanisms and potential clinical application a novel lncRNA, retinoblastoma associated transcript-1 (RBAT1), tumorigenesis. Methods RBAT1 expression was determined by real-time PCR both (Rb) bladder cancer (BCa) cell lines...
Abstract Background Parkinson’s disease (PD) is characterized by degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc), accompanied accumulation α-synuclein, chronic neuroinflammation and autophagy dysfunction. Previous studies suggested that misfolded α-synuclein induces inflammatory response dysfunction microglial cells. The NLRP3 inflammasome signaling pathway plays a crucial role neuroinflammatory process central nervous system. However, relationship between...
Rationale: Angiogenesis is a fundamental process of tumorigenesis, growth, invasion and metastatic spread.Extracellular vesicles, especially exosomes, released by primary tumors promote angiogenesis cancer progression.However, the mechanism underlying pro-angiogenic potency cell-derived exosomes remains poorly understood.Methods: Exosomes were isolated from breast cells with high potential (HM) low (LM).The effects these evaluated in vitro tube formation assays, wound healing rat arterial...
We used molecular dynamics simulation to study the effect of external structure on water permeation across a single-walled nanochannel. In contrast with macroscopic scenario, outside greatly affects transport Remarkably, ratio maximal minimal flux reached value about two for different structures. These findings are expected be helpful in design high-flux nanochannels and provide an insight into contribution lipid membrane biological channels.
// Yangyang Hu 1, 2 , Chao Deng 1 He Zhang Jing Bo Peng and Chuanyi Department of Urology, Gongli Hospital, The Second Military Medical University, Shanghai 200135, China First Clinical College, Nanjing 211166, Correspondence to: Hu, email: chuanyihuglyy@163.com Peng, pengbo6908@163.com Keywords: lncRNA, XIST, bladder cancer, miR-139-5p, Wnt1 Received: August 24, 2017 Accepted: September 22, Published: October 10, ABSTRACT Bladder cancer is one the most common urological malignancy all over...
Monoallelic expression of IGF2 is regulated by CCCTC binding factor (CTCF) to the imprinting control region (ICR) on maternal allele, with subsequent formation an intrachromosomal loop promoter region. The N-terminal domain CTCF interacts SUZ12, part polycomb repressive complex-2 (PRC2), silence allele. We synthesized decoy proteins, fusing deoxyribonucleic acid-binding zinc finger CpG methyltransferase Sss1 or enhanced green fluorescent protein. In normal human fibroblasts and breast cancer...
Increasing evidence suggests that aberrant long non-coding RNAs (lncRNAs) are significantly correlated with the pathogenesis, development and metastasis of cancers. RHPN1 antisense RNA 1 (RHPN1-AS1) is a 2030-bp transcript originating from human chromosome 8q24. However, role RHPN1-AS1 in uveal melanoma (UM) remains to be clarified. In this study, we aimed elucidate molecular function UM. The levels UM cell lines were examined using quantitative real-time polymerase chain reaction (qRT-PCR)....
Exercise can increase skeletal muscle sensitivity to insulin, improve insulin resistance and regulate glucose homeostasis in rat models of type 2 diabetes. However, the potential mechanism remains poorly understood. In this study, we established a male Sprague–Dawley model diabetes, with β cell dysfunction, which was induced by high-fat diet low-dose streptozotocin replicate pathogenesis metabolic characteristics diabetes humans. We also investigated possible chronic acute exercise improves...