Fusarium verticillioides, an important maize pathogen produces fumonisins and causes stalk ear rot; thus, we are aimed to clarify its infection cycle by assessing enhanced green fluorescent protein (EGFP) expression in rot strains. Maize seeds were inoculated with stable strongly pathogenic transformants. To investigate the degree of infection, plants observed under a stereo fluorescence microscope, affected tissue strains detected using PCR. We found that both transformants infected maize....

Triple-negative breast cancer (TNBC), characterized by the lack of expression estrogen receptor, progesterone and human epidermal growth factor receptor 2, is an aggressive form that conveys unpredictable poor prognosis due to limited treatment options effective targeted therapies. Wnt/β-catenin signaling hyperactivated in TNBC, which promotes progression TNBC. However, molecular mechanism activation TNBC remains unknown. Here, we report drastic overexpression miR-221/222 all four cell lines...

A bioenergetic balance between glycolysis and mitochondrial respiration is particularly important for stem cell fate specification. It however remains to be determined whether undifferentiated spermatogonia switch their preference bioenergy production during differentiation. In this study, we found that ATP generation in was gradually increased upon retinoic acid (RA)-induced To accommodate elevated energy demand, RA signaling concomitantly switched from respiration, accompanied by levels of...

Exercise can increase skeletal muscle sensitivity to insulin, improve insulin resistance and regulate glucose homeostasis in rat models of type 2 diabetes. However, the potential mechanism remains poorly understood. In this study, we established a male Sprague–Dawley model diabetes, with β cell dysfunction, which was induced by high-fat diet low-dose streptozotocin replicate pathogenesis metabolic characteristics diabetes humans. We also investigated possible chronic acute exercise improves...

Although mitochondrial morphology is well-known for its role in cellular homeostasis, there surprisingly little knowledge on whether remodeling required postnatal germ cell development. In this study, we investigated the functions of MFN1 and MFN2, two GTPases fusion, during early spermatogenesis. We observed increased MFN expressions along with endoplasmic reticulum (ER) activities spermatogonial differentiation. Deletion either Mfn led to DNA oxidation apoptosis specifically...

The continuous cropping can restrict the large scale and intensive cultivation of muskmelon, use Trichoderma preparation to alleviate negative effects is an effective mean. Although impact on rhizosphere soil microbial communities metabolites after applying are still unclear. In this study, we applied fermentation broth viride T23 muskmelon under cropping, collected samples at 60 days transplantation, investigated changes in by using high−throughput sequencing metabolomic analysis,...

The limitations and weaknesses of continuous melon cropping have worsened in recent years. A melon–broccoli rotation can possibly alleviate the problems associated with monoculture; however, underlying mechanisms their impact on rhizosphere’s soil microbial community remain unclear. Thus, high-throughput sequencing was used to evaluate rhizosphere soil’s community’s relative abundance diversity under monoculture systems. We found that fungal bacterial richness increased while fungi...

Exploring novel antimicrobial drugs and strategies has become essential to the fight MRSA-associated infections. Herein, we found that membrane-disrupted repurposed antibiotic salifungin had excellent bactericidal activity against MRSA, with limited development of drug resistance. Furthermore, adding effectively decreased minimum inhibitory concentrations clinical antibiotics Staphylococcus aureus. Evaluations mechanism demonstrated disrupted level H+ K+ ions using hydrophilic lipophilic...

Alfalfa (Medicago sativa L.) is an important and widely cultivated forage legume, yet its yield constrained by salinity stress. In this study, we characterized R2R3-MYB transcription factor MsEOBI in alfalfa. Its salt tolerance function regulatory pathways were investigated. The nuclear-localized functions as a transcriptional activator, enhancing promoting the biosynthesis of flavonoids lignin, well facilitating scavenging reactive oxygen species (ROS). Additionally, promotes pollinator...

Wnt2 plays a pivotal role in human cancer. However, it is not yet known whether contributes to promoting migration and invasion of gastric The present study aimed investigate the expression cancer tissues cell lines, analyze effect on cells. level was evaluated samples adjacent normal by immunohistochemical western blot analysis. To cells, an vitro secreting system established using CHO results indicated that overexpressed implicated metastasis TNM stage. Additionally, experiments...

Glaesserella parasuis (G. parasuis) causes inflammation and damage to piglets. Whether polyserositis caused by G. is due tight junctions the protective effect of baicalin on it have not been examined. Therefore, this study aims investigate effects peritoneal piglets challenged with its underlying molecular mechanisms. Piglets were treated or without baicalin. RT-PCR was performed examine expression genes. Immunofluorescence carried out detect distribution patterns proteins. Western blot...

Highlights•Accelerated mitochondrial dynamics promote spermatogonial differentiation•DRP1 deficiency in germ cells blocks differentiation•MFN1 promotes metabolism differentiating spermatogonia•DRP1 regulates permeability pore spermatogoniaSummaryAt different stages of spermatogenesis, cell mitochondria differ remarkably morphology, architecture, and functions. However, it remains elusive how change their features during differentiation, which turn impacts stem fate decision. In this study,...

Large-scale bottom-up proteomics of few even single cells is crucial for a better understanding the roles played by cell-to-cell heterogeneity in disease and development. Novel proteomic methodologies with extremely high sensitivity are required single-cell proteomics. Sample processing recovery no contaminants one key step. Here we developed nanoparticle-aided nanoreactor nanoproteomics (Nano3) technique low-nanograms mammalian cell proteins proteome profiling. The Nano3 employed...

Highly virulent Streptococcus suis (S. suis) infections can cause Streptococcal toxic shock-like syndrome (STSLS) in pigs and humans, which an excessive inflammatory response causes severe damage. Hemolysin (SLY) is a major virulence factor of S. serotype 2 that produces pores the target cell membrane, leading to cytoplasmic K+ efflux activation NLRP3 inflammasome, ultimately causing STSLS. The critical aspect hemolysin pathogenesis type makes it attractive for development innovative...

The autotoxins of muskmelon are one the most important reasons for continuous cropping obstacle muskmelon, which main components phenolic acids. Phenolic acids can inhibit growth and development plants. purpose this study was to screen strains that degrade in soil. Using as sole carbon source, were isolated screened by dilution plate method, could efficiently various abilities measured HPLC, effects degrading soil verified a pot experiment. After identification, strain T58 identified...

The aim of the present study was to investigate effect and mechanism triiodothyronine (T3) on alcoholic liver disease (ALD)-induced injuries in mice. A total 40 male C57/BL6 mice were randomly divided into Control, ALD, ALD+T3 ALD+T3+AMP-activated protein kinase inhibitor (CC) groups. Mice administered alcohol (4 g/kg/day) intragastrically for 4 weeks except Control group. group given T3 (0.1 mg/kg/day) while ALD+T3+CC CC (10 1 week. ALD groups treated with saline. Liver tissue blood samples...

Glaesserella parasuis ( G. ) can cause Glässer's disease and severely affect swine industry worldwide. This study is an attempt to address the issue of capability damage vascular barrier effects baicalin on tight junctions (TJ) in order investigate interactions between pathogen porcine endothelium. Piglets were challenged with treated or without baicalin. The expressions TJ genes examined using RT-PCR. distribution patterns proteins detected by immunofluorescence. involved signaling pathways...

Tuberculosis and drug-resistant TB remain serious threats to global public health. It is urgent develop novel anti-TB drugs in order control it. In addition redesigning developing new drugs, drug repurposing also an innovative way antibacterial drugs. Based on this method, we discovered SKQ-1 the FDA-approved library evaluated its activity. vitro, demonstrated that engaged bactericidal activity against drug-sensitive -resistant Mtb confirmed synergistic effects of SKQ1 with RIF INH....

Alfalfa ( Medicago sativa L.) is a crucial and widely grown forage legume that faces yield challenges due to salinity stress. In this study, an R2R3-MYB transcription factor (TF), MsEOBI was characterized from alfalfa. Its function gene regulatory pathways were studied in alfalfa seedlings. The nuclear-localized acts as transcriptional activator. enhances tolerance of by promoting flavonoids lignin biosynthesis, well scavenging reactive oxygen species (ROS). also promote pollinator...