Entamoeba histolytica es el parásito causante de la amibiasis, este organismo invade intestino grueso y genera lesiones a nivel colon puede propagarse manera sistémica otros órganos como hígado en donde da lugar formación del absceso hepático amibiano, condiciones clínicas graves los pacientes pueden llegar muerte. Los reportes OMS indican que afecta principalmente personas países vías desarrollo; México, se estima una prevalencia 40% población. El tratamiento más utilizado contra amibiasis...

Pterygium is a disorder of the ocular surface induced by chronic exposure to UV-light. Abundant data available from patients with primary pterygium, but scarce those recurrent pterygium. The present study aimed explore oxidant/antioxidant status in tissue and pterigium men women.Pathological samples were taken during surgery on Healthy conjunctive cataract surgery. After homogenization 77 samples, evaluation was made thiobarbituric reactive substances (TBARS), nitric oxide (NO), total...

Naegleria fowleri infects humans through the nasal mucosa causing a disease in central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play critical role early phase of N. infection. Recently, new biological defence mechanism called neutrophil extracellular traps (NETs) has been attracting attention. NETs are composed nuclear DNA combined with histones and antibacterial proteins, these structures released from cell to direct its antimicrobial...

Amoebic liver abscess (ALA) is the most important extraintestinal complication of Entamoeba histolytica infection. development causes severe destruction tissue concomitant with a strong inflammatory reaction. We analyse in situ expression TNF-α, IFN-γ, IL-1β, 1L-8 and IL-10 at different stages ALA susceptible animal model. Results showed that after inoculation, neutrophils (PMN) some macrophages infiltrated were positive for TNF-α IFN-γ acute phase amoeba The presence these cytokines was...

Entamoeba histolytica invades the intestine and other organs during pathogenesis of amoebiasis. In early stages, host organism responds with an inflammatory infiltrate composed mostly neutrophils. It has been reported that these immune cells, activated by E. histolytica, exert a protective role releasing proteolytic enzymes generating reactive oxygen/nitrogen species (ROS/RNS) antimicrobial peptides. is now known neutrophils also produce neutrophil extracellular traps (NETs), which are able...

During amebic invasion, neutrophils are a key component in either protecting against invading trophozoites or contributing to tissue damage. Upon degranulating being lysed, release toxic substances that can kill amebas as well damage host tissue. In previous study we identified protein from nonspecifically stimulated peritoneal exudates of hamster has peroxidase and marked amebicidal activity. the current analyzed vitro effect purified myeloperoxidase (MPO). The results demonstrate MPO must...

Abstract The impact of intermittent fasting versus ad libitum feeding during S almonella typhimurium infection was evaluated in terms duodenum I g A levels, bacterial clearance and intestinal extra‐intestinal susceptibility. Mice that were intermittently fasted for 12 weeks or fed infected with . assessed at 7 14 days post‐infection. Next, we load the faeces, P eyer's patches, spleen liver by plate counting, as well total specific plasmatic corticosterone levels (by immunoenzymatic assay)...

The intestinal epithelium is a monolayer of cells arranged side‑by‑side and connected by tight junction (TJ) proteins expressed at the apical extreme paracellular membrane. This layer prevents stress‑induced inflammatory responses, thus helping to maintain gut barrier function homeostasis. aim present study was evaluate effects chronic immobilization stress on colonic expression various parameters A total two groups female BALB/c mice (n=6) were included: stressed group (short‑term for 2...

Amoebiasis is produced by the parasite Entamoeba histolytica; this disease affects millions of people throughout world who may suffer from amoebic colitis or liver abscess. Metronidazole used to treat protozoan, but it causes important adverse effects that limit its use. Studies have shown riluzole has demonstrated activity against some parasites. Thus, present study aimed, for first time, demonstrate in vitro and silico anti-amoebic riluzole. In vitro, results histolytica trophozoites...

Hypermutated high-affinity immunoglobulin A (IgA), neutralizes toxins and drives the diversification of bacteria communities to maintain intestinal homeostasis although mechanism underlies impact moderate aerobic exercise (MAE) on IgA-generation via T-dependent (TD) is not fully know. Therefore, aim this study was determine effect long-time MAE production IgA through TD pathway in Peyer´s patches small intestine from aged mice. protocol consisted twenty 3-month-old (young) BALB/c mice...

The protozoan Entamoeba histolytica is the etiological agent of amoebiasis, which can spread to liver and form amoebic abscesses. Histological studies conducted with resistant susceptible models abscesses (ALAs) have established that neutrophils are first cells contact invasive amoebae at lesion site. Myeloperoxidase most abundant enzyme secreted by neutrophils. It uses hydrogen peroxide same oxidize chloride ions produce hypochlorous acid, efficient microbicidal system In a previous report,...

The main effector mechanisms of neutrophils are the release neutrophil extracellular traps (NETs) and myeloperoxidase (MPO). In this work, we evaluated role NETs activity MPO in interactions rodent with amoebae amoebic liver abscess (ALA)-resistant ALA-susceptible models. We showed vitro assays that mice produced greater amounts than did hamsters, elastase was high both However, inhibition promoted an increase amoeba viability mice. mouse ALAs a more profound presence hamster ALAs. concluded...

is a protozoan-pathogen-causing amoebic liver abscess (ALA). After amoeba establishment in the liver, it causes abundant infiltrate of neutrophils. Liver tissue damage by neutrophils results part from anti-amoebic oxidative intermediates, including reactive oxygen species (ROS), nitrogen (RNS), and hypochlorous acid (HOCl), derived myeloperoxidase (MPO) enzyme. Ascorbic (ASC) an antioxidant that acts as scavenger for ROS NOS-derived free radicals. No previous information regarding effect ASC...

Naegleria fowleri is a free-living amoeba, which able to infect humans through the nasal mucosa causing disease in central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play critical role early phase of N infection. Recently, new biological defence mechanism called neutrophil extracellular traps (NETs) has been attracting attention. These structures represent an important strategy immobilize and kill invading microorganisms. In this work,...