A5069059354- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Infectious Diseases and Tuberculosis
- Antibiotic Resistance in Bacteria
- Diagnosis and treatment of tuberculosis
- vaccines and immunoinformatics approaches
- Evolution and Genetic Dynamics
- Yersinia bacterium, plague, ectoparasites research
- Image Retrieval and Classification Techniques
- Radiomics and Machine Learning in Medical Imaging
- Pneumonia and Respiratory Infections
- RNA and protein synthesis mechanisms
European Respiratory Society
2023
Biomechanics Institute of Valencia
2018-2023
Instituto de Biomedicina de Valencia
2019-2023
Başkent University Hospital
2023
Universitat de Barcelona
2023
Barcelona Institute for Global Health
2023
Consejo Superior de Investigaciones Científicas
2019
Abstract Polyclonal infections occur when at least two unrelated strains of the same pathogen are detected in an individual. This has been linked to worse clinical outcomes tuberculosis, as undetected with different antibiotic resistance profiles can lead treatment failure. Here, we examine amount polyclonal sputum and surgical resections from patients tuberculosis country Georgia. For this purpose, sequence analyse genomes Mycobacterium isolated samples, acquired through observational study...
Genetic differences between different Mycobacterium tuberculosis complex (MTBC) strains determine their ability to transmit within host populations, latency times, and drug resistance profiles. Said usually emerge through de novo mutations are maintained or discarded by the balance of evolutionary forces. Using a dataset ∼5,000 representing global MTBC diversity, we determined past present selective forces that have shaped current variability observed in pathogen population. We identified...
Understanding why some multidrug-resistant tuberculosis cases are not detected by rapid phenotypic and genotypic routine clinical tests is essential to improve diagnostic assays advance toward personalized treatment. Here, we combine whole-genome sequencing with single-colony phenotyping identify a strain that had infected patient for 9 years. Our investigation revealed the failure of testing genome-based prediction tools strain. The false-negative findings were caused uncommon rifampicin...
The occurrence of multiple strains a bacterial pathogen such as M. tuberculosis or C. difficile within single human host, referred to mixed infection, has important implications for both healthcare and public health. However, methods detecting it, especially determining the proportion identities underlying strains, from WGS (whole-genome sequencing) data, have been limited. In this paper we introduce SplitStrains, novel method addressing these challenges. Grounded in rigorous statistical...
Tuberculosis (TB) surveillance is scarce in most African countries, even though it the continent with greatest disease incidence according to World Health Organization. Liberia within 30 countries highest TB burden, probably as a consequence of long civil war and recent Ebola outbreak, both crippling health system depreciating prevention control programmes. Due difficulties working country, there lack resistance surveys bacillus characterization. Here, we use genome sequencing Mycobacterium...
Abstract Efforts to eradicate tuberculosis are hampered by the rise and spread of antibiotic resistance. Several large-scale projects have aimed specifically link clinical mutations resistance phenotypes, but they were limited in both their explanatory predictive powers. Here, we combine functional genomics phylogenetic associations using strain genomes decipher architecture isoniazid search for new determinants. This approach has allowed us confirm main target route antibiotic, determine...
Outbreak strains of Mycobacterium tuberculosis are promising candidates as targets in the search for intrinsic determinants transmissibility, they responsible many cases with sustained transmission; however, use low-resolution typing methods and restricted geographical investigations represent flaws assessing success long-lived outbreak strains. We can now address nature by combining large genomic data sets phylodynamic approaches. retrospectively sequenced whole genome representative...
Abstract Efforts to eradicate tuberculosis are hampered by the rise and spread of antibiotic resistance. Several large-scale projects have aimed specifically link clinical mutations resistance phenotypes, but they were limited in both their explanatory predictive powers. Here, we combine functional genomics phylogenetic associations using strain genomes decipher architecture isoniazid search for new determinants. This approach has allowed us confirm main target route antibiotic, determine...
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Abstract Background Outbreak strains are good candidates to look for intrinsic transmissibility as they responsible a large number of cases with sustained transmission. However, assessment the success long-lived outbreak has been flawed by use low-resolution typing methods and restricted geographical investigations. We now have potential address nature combining genomic datasets phylodynamic approaches. Methods retrospectively sequenced whole genome representative samples assigned an...
Abstract The occurrence of multiple strains a bacterial pathogen such as M. tuberculosis or C. difficile within single human host, referred to mixed infection, has important implications for both healthcare and public health. However, methods detecting it, especially determining the proportion identities underlying strains, from WGS (whole-genome sequencing) data, have been limited. In this paper we introduce SplitStrains , novel method addressing these challenges. Grounded in rigorous...