A5075747954- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Autoimmune and Inflammatory Disorders Research
- Otitis Media and Relapsing Polychondritis
- Cancer Genomics and Diagnostics
- Immunodeficiency and Autoimmune Disorders
- Single-cell and spatial transcriptomics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer Cells and Metastasis
- Acute Myeloid Leukemia Research
- Fibroblast Growth Factor Research
- Celiac Disease Research and Management
- Digestive system and related health
New York Genome Center
2023-2024
Cornell University
2023-2024
Columbia University Irving Medical Center
2024
Columbia University
2022-2023
Weill Cornell Medicine
2023
Center for Digestive and Liver Diseases
2022
Clinical outcomes in colorectal cancer (CRC) correlate with T cell infiltrates, but the specific contributions of heterogenous types remain unclear. To investigate diverse function cells CRC, we profiled 37,931 from tumors and adjacent normal colon 16 patients CRC respect to transcriptome, TCR sequence, surface markers. Our analysis identified phenotypically functionally distinguishable effector types. We employed single-cell gene signatures these subsets query TCGA database assess their...
Celiac disease (CD) is an autoimmune in which intestinal inflammation induced by dietary gluten. The means through gluten-specific CD4
Abstract Somatic evolution leads to the emergence of clonal diversity across tissues with broad implications for human health. A striking example somatic is VEXAS (Vacuoles E1 enzyme X-linked Autoinflammatory Somatic) syndrome, caused by UBA1 mutations in hematopoietic stem cells (HSCs), inducing treatment-refractory, systemic inflammation. However, mechanisms that lead survival and expansion mutant HSCs are unknown, limiting development effective therapies. The lack animal or cellular...
Somatic mosaicism is a hallmark of malignancy that also pervasively observed in human physiological aging, with clonal expansions cells harboring mutations recurrently mutated driver genes. Bulk sequencing tissue microdissection captures mutation frequencies, but cannot distinguish which co-occur the same clones to reconstruct architectures, nor phenotypically profile populations delineate how impact cellular behavior. To address these challenges, we developed single-cell...
Despite the widespread use and success of immune checkpoint blockade (ICB) therapies targeting negative costimulatory proteins CTLA4 PD1, means through which they affect T cells remain poorly understood. Notably, cell populations that these act upon unknown. Through single-cell TCR-seq, RNA-seq CITE-seq coupled with surgical biopsy, we longitudinally tracked fate clones within tumors in response to ICB immunogenic mouse models cancer. In this context, found both acted distinct complementary...