A5017046492- Genetics, Aging, and Longevity in Model Organisms
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Signaling Pathways in Disease
- Acute Lymphoblastic Leukemia research
- CRISPR and Genetic Engineering
Weill Cornell Medicine
2025
Cornell University
2025
New York Genome Center
2024
New York University
2021-2022
High transmissibility is a hallmark of the Omicron variant SARS-CoV-2. Understanding molecular determinants Omicron’s will impact development intervention strategies. Here we map electrostatic potential surface Spike protein to show that major SARS-CoV-2 variants have accumulated positive charges in solvent-exposed regions protein, especially its ACE2-binding interface. Significantly, Spike-ACE2 complex has complementary surfaces. In contrast, interfaces between and neutralizing antibodies...
LOTUS and Tudor domain containing proteins have critical roles in the germline. Proteins that contain these domains, such as Tejas/Tapas Drosophila, help localize Vasa helicase to germ granules facilitate piRNA-mediated transposon silencing. The homologous mammals, TDRD5 TDRD7, are required during spermiogenesis. Until now, both domains Caenorhabditis elegans remained elusive. Here we describe LOTR-1 (D1081.7), which derives its name from domains. Interestingly, docks next P colocalize with...
Over 80% of children with acute lymphoblastic leukemia (pALL) can be cured by treating them multiple chemotherapeutic agents administered over several years, whereas pALL is incurable 1-3 medications, suggesting significant variation in drug susceptibility across clonal populations. While bulk sequencing studies indicate that cells contain relatively few genetic variants compared to other cancers, the true extent diversity at single-cell level remains unknown. Here, we used three...
We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P condensation, form granules independently of in postembryonic line, balance each other regulating growth localization. MIP-2 contain two LOTUS domains regions homo- heterodimers. They bind anchor Vasa homolog GLH-1 within are jointly required for coalescence MEG-3, GLH-1, PGL proteins. Animals lacking show...
Somatic mosaicism is a hallmark of malignancy that also pervasively observed in human physiological aging, with clonal expansions cells harboring mutations recurrently mutated driver genes. Bulk sequencing tissue microdissection captures mutation frequencies, but cannot distinguish which co-occur the same clones to reconstruct architectures, nor phenotypically profile populations delineate how impact cellular behavior. To address these challenges, we developed single-cell...
Abstract High transmissibility is a hallmark of the Omicron variant SARS-CoV-2. Understanding molecular determinants Omicron’s will impact development intervention strategies. Here we map electrostatic potential surface Spike protein to show that major SARS-CoV-2 variants have accumulated positive charges in solvent-exposed regions protein, especially its ACE2-binding interface. Significantly, Spike-ACE2 complex has complementary surfaces. In contrast, interfaces between and neutralizing...
Abstract Individual bulk tumor biopsies are invasive and may fail to capture clonal heterogeneity within primary tumors metastatic sites of disease. Further, analyses subclonal variants often hindered by background error rates related tissue preservation methods (e.g. FFPE) sequencing artifact. Use plasma-only ultrasensitive liquid biopsy approaches allow for noninvasive inference clonality dynamically track changes in burden. We previously developed MRD-EDGE, a tumor-informed machine...
Abstract We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P condensation, form granules independently of in postembryonic line, balance each other regulating growth localization. MIP-2 contain two LOTUS domains regions homo- heterodimers. They bind anchor Vasa homolog GLH-1 within are jointly required for coalescence MEG-3, GLH-1, PGL proteins. Animals lacking show...
Abstract LOTUS and Tudor domain containing proteins have critical roles in the germline. Proteins that contain these domains, such as Tejas/Tapas Drosophila , help localize Vasa to germ granules facilitate piRNA-mediated transposon silencing. The homologous mammals, TDRD5 TDRD7, are required during spermiogenesis. Until now, both domains Caenorhabditis elegans remained elusive. Here we describe LOTR-1 (D1081.7), which derives its name from LO TUS T udo r domains. Interestingly, docks next P...