A5046972722- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Multiple Myeloma Research and Treatments
- Celiac Disease Research and Management
- Glycosylation and Glycoproteins Research
- Microscopic Colitis
- Cancer Cells and Metastasis
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Pancreatic function and diabetes
- RNA Research and Splicing
- Chemokine receptors and signaling
- Transplantation: Methods and Outcomes
- Fibroblast Growth Factor Research
- Immunodeficiency and Autoimmune Disorders
- Wnt/β-catenin signaling in development and cancer
- Acute Myeloid Leukemia Research
- Neonatal Respiratory Health Research
- Digestive system and related health
- Renal Transplantation Outcomes and Treatments
Columbia University Irving Medical Center
2017-2025
Columbia University
2018-2024
Center for Digestive and Liver Diseases
2022-2024
Herbert Irving Comprehensive Cancer Center
2022
Stanford University
2013-2018
Howard Hughes Medical Institute
2018
Agency for Science, Technology and Research
2013
New York University
2003
Icahn School of Medicine at Mount Sinai
2003
Rockefeller University
2003
Highlights•Development of a human leukocyte antigen library for TCR ligand identification•Single-cell sequencing and phenotyping T cells infiltrating colon cancer•Ligand discovery four tumor-derived cell receptors•Identification shared non-mutated tumor between two patientsSummaryThe immune system can mount responses against tumors; however, the specificities tumor-infiltrating lymphocytes (TILs) are not well understood. We used yeast-display libraries peptide-human (pHLA) to screen antigens...
γδ T cells represent a substantial fraction of intestinal lymphocytes at homeostasis, but they also constitute major lymphocyte population infiltrating colorectal cancers (CRCs); however, their temporal contribution to CRC development or progression remains unclear. Using human samples and murine models, we found that most in premalignant nontumor colons exhibit cytotoxic markers, whereas tumor-infiltrating express protumorigenic profile. These contrasting cell profiles were associated with...
Celiac disease is an intestinal autoimmune driven by dietary gluten and gluten-specific CD4 + T-cell responses. In celiac patients on a gluten-free diet, exposure to induces the appearance of T cells with gut-homing potential in peripheral blood. Here we show that also activated, CD8 αβ γδ Single-cell receptor sequence analysis indicates both these cell populations have highly focused repertoires, indicating their induction antigen-driven. These results reveal previously unappreciated role...
Clinical outcomes in colorectal cancer (CRC) correlate with T cell infiltrates, but the specific contributions of heterogenous types remain unclear. To investigate diverse function cells CRC, we profiled 37,931 from tumors and adjacent normal colon 16 patients CRC respect to transcriptome, TCR sequence, surface markers. Our analysis identified phenotypically functionally distinguishable effector types. We employed single-cell gene signatures these subsets query TCGA database assess their...
Celiac disease (CD) is an autoimmune in which intestinal inflammation induced by dietary gluten. The means through gluten-specific CD4
Interleukin (IL)-17 plays a key role in immunity. In acute infections, rapid IL-17 response must be induced without prior antigen exposure, and γδ T cells are the major initial producers. fact, some make within hours after an immune challenge. These appear to acquire ability respond IL-1 IL-23 naturally naïve animals. They known as natural Tγδ17 (nTγδ17) cells. The rapidity of nTγδ17 response, apparent lack explicit cell receptor (TCR) engagement for its induction have led view that this is...
Abstract Introduction: Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) are pathologically activated neutrophils that impair immunotherapy responses. The chemokine receptor CXCR4, a central regulator of hematopoiesis, represents an attractive PMN-MDSC target. Methods: We generated novel peptide, TFF2-MSA, as CXCR4 partial agonist by fusing Trefoil Factor 2 (TFF2) with murine serum albumin (MSA), and compared its treatment efficacy in combination anti-PD-1 antibody to the...
The CD28 cell surface receptor provides an important costimulatory signal for T cells necessary their response to Ag. Early events in signaling include recruitment and activation of phosphatidylinositol 3-kinase (PI3-kinase) the protein tyrosine kinases (PTKs), LCK EMT. Recruitment PI3-kinase is known be dependent upon phosphorylation 173 cytoplasmic tail contained within a YMNM motif. By contrast, little which residues tail, including tyrosines, are required PTKs. To address this we studied...
Abstract Multiple myeloma is characterized by the clonal expansion of malignant plasma cells in bone marrow. But phenotypic diversity and contribution less predominant B-lineage clones to biology this disease have been controversial. Here, we asked whether bearing dominant multiple immunoglobulin rearrangement occupy compartments other than that cells. To accomplish this, combined 13-parameter FACS index sorting t-Stochastic Neighbor Embedding (t-SNE) visualization with high-throughput...
Ligation of the CD2 cell surface glycoprotein expressed on T lymphocytes and NK cells induces protein tyrosine phosphorylation activation Src kinases, LCK FYN. We show here that in Jurkat leukemia peripheral blood cells, stimulation also leads to Tec family kinase, EMT/ITK/TSK. Activation EMT by was induced mitogenic pairs mAb, certain single mAb followed secondary antibody cross-linking, CD58-bearing sheep red cells. With use different mutants it demonstrated CD2-mediated required...