A5069327181- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Gene Regulatory Network Analysis
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Molecular Communication and Nanonetworks
- Neonatal Respiratory Health Research
- Microbial Metabolic Engineering and Bioproduction
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Child Nutrition and Feeding Issues
- Congenital Diaphragmatic Hernia Studies
- Mass Spectrometry Techniques and Applications
- Simulation-Based Education in Healthcare
- Eosinophilic Esophagitis
- Pediatric Hepatobiliary Diseases and Treatments
- Evolution and Genetic Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Research and Splicing
- Food Allergy and Anaphylaxis Research
- Advanced Fluorescence Microscopy Techniques
- Advanced Differential Equations and Dynamical Systems
- Developmental Biology and Gene Regulation
Duke Medical Center
2023-2025
Wuhan University
2024
Renmin Hospital of Wuhan University
2024
California Institute of Technology
2016-2023
Duke University
2023
Menlo School
2022
10X Genomics (United States)
2021
McGill University
2016
Anhui Normal University
2007-2015
Shanghai University
2007-2008
Abstract Background Virtual simulation and face-to-face are effective for clinical judgment training. Rare studies have tried to improve ability by applying virtual together. This study aimed evaluate the effect of an integrated non-immersive high-fidelity program on enhancing nursing students’ understanding experiences combined simulation. Methods A sequential exploratory mixed-methods was conducted in a center university Central China. Third-year students ( n = 122) taking training ICUs...
As early T cell precursors transition from multipotentiality to lineage commitment, they change expression of multiple transcription factors. It is unclear whether individual factors directly control choices between identity and some alternative fate or these mostly affect proliferation survival during the normal commitment process. Here, we unraveled impacts deleting at two stages in development, using synchronized vitro differentiation systems, single-cell RNA-seq with batch indexing,...
Significance T-lineage specification requires waves of gene network changes to terminate multipotency and establish T identity. Circumstantial evidence has suggested roles for Runt domain-related (Runx) factors in these regulatory events; Runx1 is strongly expressed, but knockouts have previously shown little impact during stages around commitment. We show that Runx3 function together drive commitment, binding the same sites. Double block both activation genes repression progenitor genes....
Notch signaling is the dominant intercellular input during earliest stages of T cell development in thymus. Although Notch1 known to be indispensable, we show that it does not mediate all precommitment stages: Notch2 initially works parallel promote early murine and antagonize other fates. Notch-regulated target genes before after lineage commitment change dynamically, this partially reflects shifts genome-wide DNA binding by RBPJ, transcription factor activated complex formation with...
Introduction Genetic testing is often pursued when evaluating patients with suspected inborn errors of immunity but interpreting variants uncertain significance (VUS) remains challenging. Functional assays are an important avenue to characterize variant pathogenicity infrequently commercially available. We report a single case in which research-based functional was critical determining pathogenicity. Case Report A 12-year-old male history recurrent childhood infections presented episodes...
Intrathymic development of committed progenitor (pro)-T cells from multipotent hematopoietic precursors offers an opportunity to dissect the molecular circuitry establishing cell identity in response environmental signals. This transition encompasses programmed shutoff stem/progenitor genes, upregulation T specification proliferation, and ultimately commitment. To explain these features light reported cis-acting chromatin effects experimental kinetic data, we develop a three-level dynamic...
The binomial τ-leaping method of simulating the stochastic time evolution in a reaction system uses random number to approximate events. Theory implies that this can avoid negative molecular numbers simulations when larger step τ is used. Presented here modified leap for improving accuracy and application range method. maximum existing population first defined approach order determine better bound reactions. To derive general procedure chemically reacting systems, new sampling based on...
Synergistic transcription activation is a key component in the generation of spectrum eukaryotic promoter activities by limited number factors. Various mechanisms could account for synergy, but central question remains whether synergism requires factor functions that differ from those direct independent activation. The rat growth hormone synergistically activated pituitary-specific factor, Pit-1, and thyroid receptor (TR). Mutations disrupted previously described DNA binding transcriptional...
Summary Recent single cell experiments have revealed significant heterogeneities at the levels of transcription, DNA methylation and chromosome organization in individual cells. However, existing method profiling mRNAs effectively averages transcriptional dynamics over many hours due to hours-long life time most mRNAs. To capture instantaneous activity transcriptome that reflects rapid regulatory changes cells, we imaged up 10,421 nascent transcription active sites (TAS) mouse embryonic stem...
BackgroundOral food challenge (OFC) is the criterion standard for diagnosing allergy (FA). It important to have parameters aid in selecting ideal OFC candidates.ObjectiveWe sought characterize outcomes and predictors of OFCs common allergens.MethodsWe completed a retrospective chart review all IgE-mediated FA performed at Duke University pediatric clinics from June 2017 through May 2022. Patients were deemed eligible milk, egg, nut if testing revealed specific IgE level not exceeding 2 kU/L...
Abstract Runx factors are essential for lineage specification of various hematopoietic cells, including T lymphocytes. However, they regulate context-specific genes and occupy distinct genomic regions in different cell types. Here, we show that dynamic binding shifts early T-cell development mostly not restricted by local chromatin state but regulated dosage functional partners. co-factors compete to recruit a limited pool T-progenitors, modest increase protein availability at pre-commitment...
Abstract In early T-cell development, single cells dynamically shift expression of multiple transcription factors (TFs) during transition from multipotentiality to T-lineage commitment, but the functional roles many TFs have been obscure. Here, synchronized in vitro differentiation systems, scRNA-seq with batch indexing, and controlled gene-disruption strategies unraveled single-cell impacts perturbing individual at two stages development. Single-cell CRISPR perturbation revealed that...