A5000282192- Zebrafish Biomedical Research Applications
- Environmental Toxicology and Ecotoxicology
- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Animal testing and alternatives
- Microplastics and Plastic Pollution
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Computational Drug Discovery Methods
- Cell Image Analysis Techniques
- Circadian rhythm and melatonin
- Per- and polyfluoroalkyl substances research
- Renal cell carcinoma treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- Molecular Biology Techniques and Applications
- Medical Imaging and Pathology Studies
- Fish Ecology and Management Studies
- Neuroscience and Neuropharmacology Research
- Renal and Vascular Pathologies
- Systemic Sclerosis and Related Diseases
- Bioinformatics and Genomic Networks
- Digital Imaging for Blood Diseases
- Testicular diseases and treatments
- Mass Spectrometry Techniques and Applications
Vrije Universiteit Amsterdam
2014-2023
University of Amsterdam
2021
RWTH Aachen University
2018
Society of Environmental Toxicology and Chemistry
2009-2017
Karlsruhe Institute of Technology
2009-2016
Environmental Health
2009
FZI Research Center for Information Technology
2007
Semmelweis University
1976-1978
During early development, neurotransmitters are important stimulants for the development of central nervous system. Although different neuronal cell types during zebrafish (Danio rerio) is well-studied, little known levels neurotransmitters, their precursors and metabolites how these affected by exposure to environmental contaminants. A method based on hydrophilic interaction liquid chromatography coupled tandem mass spectrometry has been applied first time embryos larvae study five...
Methyl mercury (MeHg) is a neurotoxicant with adverse effects on the development of nervous system from fish to man. Despite detailed understanding molecular mechanisms by which MeHg affects cellular homeostasis, it still not clear how causes developmental neurotoxicity. We performed here genome-wide transcriptional analysis MeHg-exposed zebrafish embryos and combined this whole-mount in situ expression 88 MeHg-affected genes. The majority analyzed genes showed tissue- region-restricted...
Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) are of growing concern, as they have been detected in both humans and wildlife shown to be toxic. Recent studies indicated that OH-PBDEs can more toxic than PBDEs, partly due their ability disrupt oxidative phosphorylation (OXPHOS), an essential process energy metabolism. In this study, we determined the OXPHOS disruption potential 18 OH-PBDE congeners reported marine using two vitro bioassays, namely classic rat mitochondrial...
PFHxS (Perfluorohexane sulfonic acid) is one of the short-chain perfluoroalkyl substances (PFASs) which are widely used in many industrial and consumer applications. However, limited information available on molecular mechanism toxicity (e.g. lipid metabolism). This study provides in-depth regulation zebrafish embryos with without exposure. Lipid changes throughout development (4 to 120 h post fertilization (hpf)) were closely associated species composition (fatty acyl chains). A...
In (eco-)toxicological studies the light/dark transition (LDT) test is one of most frequently used behaviour assays with zebrafish eleutheroembryos. However, study results vary regarding data presentation and analysis mostly focus on a limited amount recorded data. this study, we investigated whether monitoring two behavioural outcomes (time distance moved) together analysing multiple parameters can improve sensitivity interpretation. As proof principle 5-day old (Danio rerio)...
Bisphenol A (BPA) is an industrial chemical, which has raised human health and environmental concerns due to its endocrine-disrupting properties. BPA analogues are less well-studied despite their wide use in consumer products. These have been detected water aquatic organisms around the world, with some showing toxic effects various species including fish. Here, we present novel organ-specific time-course distribution data of bisphenol Z (BPZ) female zebrafish (Danio rerio), concentrations...
Perfluorohexane sulfonate (PFHxS) is one of the short-chain perfluoroalkyl substances (PFASs), and frequently detected in environment, humans, wildlife, but a detailed mechanism toxicity has been not studied yet. In this study, comprehensive set polar metabolites was determined i) developing zebrafish embryo (4, 24, 48, 72, 120 h post fertilization (hpf)), ii) after exposure to four concentrations PFHxS (0.3, 1, 3, 10 μM) from 24to hpf. The temporal (developmental stages) distribution...
Behavioral studies are important tools for understanding the development and pathology of neurological diseases. Zebrafish an emerging alternative model in behavioral as repertoire zebrafish (Danio rerio) is similar to humans, nervous system structures functions highly conserved. In this study, we investigated alterations day/night locomotor activity free swimming, feeding wild-type larvae (8-15dpf) due changes rhythm light/dark cycles or caloric content food. We furthermore exposed...
The evaluation of environmental credentials for innovative products within the research and design phase development presents a valuable yet challenging exploit. presented here aims to carry out early-stage assessment novel nano material – cellulose nanocrystals (CNC) foam by applying ex-ante life cycle (LCA) supplemented an environmental, health safety (EHS) screening. LCA is applied assess cradle-to-factory gate impacts along R&D trajectory from laboratory synthesis, conceptual design,...
Water is used in petroleum oil refineries significant volumes for cooling, steam generation and processing of raw materials. Effective water management required at to ensure their efficient responsible operation with respect the environment. However, ascertaining potential environmental risks associated discharge refinery effluents receiving waters challenging because compositional complexity. Recent European research regulatory initiatives propose a more holistic approach including...
Humans are exposed daily to complex mixtures of chemical substances via food intake, inhalation, and dermal contact. Developmental neurotoxicity is an understudied area entails one the most areas in toxicology. Animal studies for developmental (DNT) hardly performed context regular hazard studies, as they costly time consuming provide only limited information human relevance. There a need combination vitro silico tests assessment chemically induced DNT humans. The zebrafish (Danio rerio)...
This study aimed at demonstrating that effect-based monitoring with passive sampling followed by toxicity profiling is more protective and cost-effective than the current chemical water quality assessment strategy consisting of compound-by-compound analysis selected substances in grab samples. Passive samplers were deployed Dutch river delta WWTP effluents. Their extracts tested a battery bioassays chemically analyzed to obtain profiles, respectively. Chemical concentrations retrieved from...
Abstract In this study, both conventional one-dimensional liquid chromatography (1DLC) and comprehensive two-dimensional (2DLC) coupled to a high-resolution time-of-flight mass spectrometer (HR-TOF MS) were used for full-scale lipid characterization of extracts from zebrafish embryos. We investigated the influence on annotated lipids different separation mechanisms (HILIC, C18, PFP), their orders arranged in first second dimensions. As result, number species by LC-MS was between 212 448....
Abstract Background The difficulty of finding new treatments for neurological diseases with great impact in our society like Alzheimer’s disease can be ascribed part to the complexity nervous system and lack quick cost-effective screening tools. Such tools could not only help identify potential novel treatments, but also used test environmental contaminants their cause neurotoxicity. It has been estimated that 5–10% anthropogenic chemicals are developmental neurotoxic (DNT) exposure DNT...