A5013846051- Sulfur Compounds in Biology
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Amino Acid Enzymes and Metabolism
- Redox biology and oxidative stress
- Neuropeptides and Animal Physiology
- Biochemical effects in animals
- Estrogen and related hormone effects
- Cholesterol and Lipid Metabolism
- Menopause: Health Impacts and Treatments
- Coenzyme Q10 studies and effects
- Stress Responses and Cortisol
- Drug Transport and Resistance Mechanisms
- Free Radicals and Antioxidants
- PARP inhibition in cancer therapy
- Receptor Mechanisms and Signaling
- Electron Spin Resonance Studies
- Neurogenesis and neuroplasticity mechanisms
- Computational Drug Discovery Methods
- Drug Solubulity and Delivery Systems
- Nitric Oxide and Endothelin Effects
- Advancements in Transdermal Drug Delivery
- Metabolism and Genetic Disorders
- Tryptophan and brain disorders
- Genomics, phytochemicals, and oxidative stress
Ege University
2012-2024
Biruni University
2014-2015
Bursa Uludağ Üni̇versi̇tesi̇
2004
Marmara University
1990
The underlying mechanisms of Alzheimer's Disease (AD) are still unclear. It is suggested that poly(ADP-ribose) polymerase-1 (PARP-1) overactivation can cause neuroinflammation and cell death. In this study we searched the effects nicotinamide (NA), endogenous PARP-1 inhibitor, on oxidative stress, apoptosis, regulation nuclear factor kappa B (NF-κB) in amyloid beta peptide (1-42) (Aβ(1-42))-induced neurodegeneration. Sprague-Dawley rats were divided into four groups as control, Aβ(1-42),...
Resveratrol, a natural phenolic compound, has been shown to prevent cardiovascular diseases and cancer exhibit neuroprotective effects. In this study, we examined the antioxidant effects of resveratrol against hydrogen peroxide in embryonic neural stem cells. Hydrogen treatment alone increased catalase glutathione peroxidase activities but did not change superoxide dismutase levels compared with + treatment. Nitric oxide synthase activity concomitant nitric response Conversely, decreased...
Oxidative stress is a major brain injury mechanism after ischemic stroke. 12/15-lipoxygenase (12/15-LOX) key mediator of oxidative stress, contributing to neuronal cell death and vascular leakage. Nonetheless, the leading its upregulation currently unknown. We show here that Signal Transducers Activators Transcription (STATs), specifically STAT6 possibly STAT1, increase transcription 12/15-LOX in cells. Both p-STAT6 -1 bound specific STAT binding sites mouse promoter. Small interfering RNA...
Alzheimer's disease (AD) is the most common form of dementia and characterized by presence senile plaques neurofibrillary tangles, along with synaptic loss. The underlying mechanisms AD are not clarified yet, but oxidative stress mitochondrial dysfunction important factors. Overactivation poly(adenosine diphosphate ribose) polymerase‐1 (PARP‐1) enzyme has been known to cause neuroinflammation cell death in neurodegenerative processes. aim present study was investigate protective effects...
Abstract The antioxidant activity of some compounds buffer the free radicals generated either endogenously or exogenously, thus decreasing potential damage mediated by oxidation. Recent studies documented that raloxifene has properties in vitro . However, there are limited animal available to show raloxifene's properties. We aimed investigate effects on enzymes such as SOD, CAT and GPX, TrxR levels GSH MDA heart, liver brain cortex ovariectomized female rats. Female Sprague Dawley rats...
J. Neurochem. (2011) 118 , 248–255. Abstract Oxidative stress is a pathway of injury that common to almost all neurological conditions. Hence, methods scavenge radicals have been extensively tested for neuroprotection. However, saving neurons alone may not be sufficient in treating CNS disease. In this study, we the cytoprotective actions glutathione precursor gamma‐glutamylcysteine ethyl ester (GCEE) brain endothelium. First, oxidative was induced human microvascular endothelial cell line...
Thioredoxin (Trx) is a multifunctional protein with redox-active disulfide/dithiol in the active site. Thioredoxin, its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both intracellular extracellular compartments. The purpose of this report was to quantify relative expression Trx rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR 2(-DeltaDeltaC(T)) method. changes...
Mefenamic acid, a non-steroidal antiinflammatory drug (NSAID), directly and dose-dependently exhibits neuroprotective activity. In our study, we investigated the effects of mefenamic acid against d-serine on oxidative stress in hippocampus, cortex cerebellum rats. Furthermore, potential inflammatory apoptotic protective effect were determined at mRNA protein levels TNF-α, IL-1β, Bcl-2 Bax. We found that significantly increased stress, inflammation- apoptosis-related molecules region specific...
Abstract Mu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was characterize MOR mRNA expression, binding, as well G protein activation mediated by these epileptic hippocampus patients with pharmacoresistant mesial temporal lobe epilepsy (TLE). In contrast autopsy samples, obtained from TLE demonstrated enhanced expression (116%). Saturation binding experiments revealed significantly higher (60%) B max values for group, whereas K d were...
Oxidative stress is a likely molecular mechanism in the neurotoxicity of kainic acid (KA), an excitotoxic substance. The aim this report was to assess effect melatonin co-treatment against KA by measuring levels Coenzyme Q10 (CoQ 10), lipid peroxidation (LPO), and Thioredoxin (Trx) mRNA rat hippocampus. male rats were divided into three groups as saline, treatment (15 mg/kg), plus (20 mg/kg). LPO CoQ10 determined high pressure liquid chromatography (HPLC) consisting fluorescence...
The aim of this study was to investigate the effect γ-Glutamylcysteine Ethyl Ester (GCEE) on levels GSH, caspase-3 activity, DNA damage and expressions Bcl-2, Bax p53 mRNAs in rat hippocampus after status epilepticus (SE) induced by systemic kainic acid (KA). male rats were divided into four groups as controls, KA (10 mg/kg), GCEE mg/kg) KA+GCEE. Glutathione (GSH) activity determined spectrophotometrically colourimetrically, respectively. mRNA quantified comet assay reverse transcription...
Antithrombotic agents and anticoagulant drugs, such as those from the heparin family, are employed in clinical settings for prevention treatment of clotting, thromboembolism, wound healing. The potency assessment antithrombotic is typically conducted using antifactor IIa assay with manual systems which time-consuming often lack repeatability. Here, we present a novel automated system that significantly enhances repeatability, attaining an outstandingly low relative standard deviation (RSD) %...
The neurodegenerative mechanisms of Alzheimer's disease (AD) are not fully understood, but it is believed that amyloid beta (Aβ) peptide causes oxidative stress, neuroinflammation, and disrupts metabotropic glutamate receptor 5 (mGluR5) signaling by interacting with cholesterol caveolin-1 (Cav-1) in pathogenic lipid rafts. This study examined the effect 2-hydroxypropyl-β-cyclodextrin (HP-CD) on cholesterol, stress (total oxidant status), neuroinflammation (TNF-α), mGluR5 molecules such as...
Glutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays key role controlling cellular thiol redox system, maintaining the immune detoxification system. The determination of GSH levels tissue important to estimate defenses against oxidative stress. In our study, multi-walled carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used determine trichloroacetic acid (TCA)-treated or untreated samples rat plasma. It was found that...
The selective estrogen receptor modulators (SERMs) are compounds that activate the receptors with different estrogenic and antiestrogenic tissue-specific effects. similar effects of SERMs on encourage efforts in research neuroprotective SERMs. In our study, potential raloxifene were investigated brain cortex ovariectomized rats after kainic acid-induced oxidative stress. To show effect against a neurodegenerative agent, acid, expression Bcl-2, total glutathione (GSH), nitrite-nitrate levels...
Abstract A possible DNA damage after interaction of kainic acid (KA) with calf thymus double stranded and genomic was herein determined in vitro vivo conditions using; electrochemical assay agarose gel electrophoresis. The changes guanine signal were detected as an indicator samples isolated from 1 or 10 mg/kg KA‐treated animals. decreased levels found 29% 33% by KA treatment when compared to controls, respectively. results electrophoresis confirmed obtained identical method.
The oxidative mechanisms of injury-induced damage neurons within the spinal cord are not very well understood. We used a model T8-T9 injury (SCI) in rat to induce neuronal degeneration. In this model, unilateral avulsion causes stress neurons. tested hypothesis that apurinic/apyrimidinic endonuclease (or redox effector factor-1, APE/Ref-1) regulates oxidation mechanism region caudal lesion, and DNA is an early upstream signal. embryonic neural stem cell therapy significantly decreased...
Inflammation is deleterious for organs with reduced capacity of regeneration, such as the brain. Recently, studies have focused on investigating therapeutic effects nonsteroidal anti-inflammatory drugs (NSAIDs) in Alzheimer’s disease, Parkinson’s Huntington’s and multiple sclerosis. Excitotoxicity pathological process when receptors excitatory neurotransmitter glutamate, N-methyl-D-aspartate (NMDA), are overactivated. This may be involved neurodegenerative diseases. D-serine one coagonist...