In this study, we have investigated the antiproliferative effect of quercetin on human papillary thyroid cancer cells and determined apoptotic mechanisms underlying its actions. We used different concentrations to induce apoptosis measured cell viability. Apoptosis cycle analysis was by flow cytometry using Annexin V propidium iodide. Finally, changes in caspase-3 cleaved poly(ADP-ribose) polymerase (PARP) protein expression levels as hallmarks Hsp90 level a marker proteasome activity...

Chronic myeloid leukemia is a major hematopoietic malignancy characterized by expansion of cells. In this study, we have investigated whether quercetin, curcumin and their combination induce apoptosis inhibit growth K562 We observed that quercetin induced accompanied increased ROS decreased GSH levels as well loss mitochondrial membrane potential. Our mRNA protein expression results suggested cytochrome c was released from mitochondria causing PARP caspase-9 cleavages, the hallmarks...

1. This study compared the effect of dietary supplementation with organic or inorganic selenium (Se) sources plus control amounts large vitamin E (α-tocopherol acetate) in broilers raised at (20 to 24°C) low (14·5 16·8°C) temperatures after 2 weeks age. 2. The following treatments were used from one day old. Diet 1, diet, comprised a commercial diet containing 0·15 mg/kg Se and 50 mg E/kg feed. was same as supplemented Se. 3 but 200 E. 4 replaced 0·30 5 4, 3. Low temperature reduced growth...

Flavonoids are phenolic substances with chemo-preventive and chemotherapeutic properties. They widely found in fruits vegetables. The polyphenols quercetin curcumin have antioxidant, anti-inflammatory, anti-carcinogenic, pro-apoptotic were successfully used against different human cancers, especially chronic myeloid leukemia cancer cells. We previously investigated anti-proliferative apoptotic effects of combination K562 Our data showed that they had beneficial synergistic effects. Based on...

Exposure to ultraviolet (UV) irradiation causes damage the skin and induces photoaging. UV stimulates production of reactive oxygen/nitrogen species, which results in activation epidermal growth factor receptor (EGFR) mitogen-activated protein kinases (MAPK) fibroblasts. MAPKs are responsible for activator protein-1 (AP-1), subsequently upregulates expression matrix metalloproteinases (MMPs). Melatonin is a potent free radical scavenger known have photoprotective effects. The aim this study...

The purpose of this study was to investigate the long- and short-term inflammatory apoptotic effects whey protein on livers non-exercising rats. Thirty rats were divided into three groups namely (1) control group, (2) (WS) diet (252 g/kg for 5 days), (3) long-term (WL) 4 weeks). Interleukin 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), cytokeratin 18 (CK-18-M30) assessed using enzyme-linked immunosorbent assay immunohistochemical methods. Apoptosis evaluated terminal...

Tissue engineering approaches which include a combination of cells and scaffold materials provide an alternative treatment for meniscus regeneration. Decellularization recellularization techniques are potential options transplantation. Maintenance the ultrastructure composition extracellular matrix repopulation with important factors in constructing biological eliminating immunological reactions. The aim study is to develop method obtain functional scaffolds For this purpose, tissue was...

The experiment was conducted to study the effects of organic selenium (Se-enriched yeast) and vitamin E (α-tocopheryl acetate) supplementation, alone or together, on performance antioxidant status broilers fed diets enriched in n-3 PUFAs using fish oil. Day-old Hubbard-JV strain broiler chicks allocated one following treatments: (1) a commercial basal diet containing 0.15 mg inorganic Se /kg as sodium selenite 50 α-tocopheryl acetate (α-TA)/kg feed acted control; (2) VE200:...

People living in Mediterranean countries are mostly exposed to solar ultraviolet (UV) radiation that damages skin and results photoaging which involves activation of epidermal growth factor receptor (EGFR) downstream signal transduction through mitogen-activated protein kinases (MAPKs) fibroblasts. Generation reactive oxygen/nitrogen species by UV is also critical for EGFR MAPKs activation. responsible AP-1 subunits the nucleus induce matrix metalloproteinases. Melatonin, along with its...

Polyphenols are present in high amounts all parts of plants including roots, seeds, flowers, leaves, branches and trunk as well plant derived products such tea, coffee wine. Extensive amount information is available on biological effects polyphenols antioxidant, anti-cancer, antiinflammatory, anti-coagulant anti-microbial activities. In recent years, researchers have turned their interest towards identifying molecular mechanisms underlying the anti-cancer these compounds. However, limited...

Oxidative stress is a likely molecular mechanism in the neurotoxicity of kainic acid (KA), an excitotoxic substance. The aim this report was to assess effect melatonin co-treatment against KA by measuring levels Coenzyme Q10 (CoQ 10), lipid peroxidation (LPO), and Thioredoxin (Trx) mRNA rat hippocampus. male rats were divided into three groups as saline, treatment (15 mg/kg), plus (20 mg/kg). LPO CoQ10 determined high pressure liquid chromatography (HPLC) consisting fluorescence...

Objective: Human SH-SY5Y cell line has been frequently used for in vitro experiments neuroscience-related research. To reflect a better neuronal characteristic the needs to differentiation. compare results obtained from models, similarity of phenotype and cells great importance. However, many studies have performed using different medium ingredients which affect differentiation progress cells. Therefore, we aimed generally mediums, contain only retinoic acid (RA) supplemented with...

Disaggregation of amyloid βeta (Aβ) is considered as one the promising therapeutic strategies for Alzheimer’s disease. Polyphenols are molecules disaggregation Aβ. However, in order to find a potential candidate, vitro analyses need be performed on model that mimics blood‑brain barrier (BBB) much possible. Therefore, we aimed establish an BBB representative transwell system by using differentiated human neuroblastoma (SH‑SY5Y), cerebral microvascular endothelial, and astrocyte cells...

Human SH-SY5Y cell line has been used as an in vitro model neuroscience research. However, many researchers emphasized that there are differences between differentiated and undifferentiated characteristics of SH-SY5Y. Although Retinoic Acid (RA) generally have for differentiation cells, which protocol provides better not cleared yet. Therefore, we compared RA the other mediums different treatment periods obtaining differentiated, carrying a neuron-like phenotype (N-type) characteristics,...

Abstract Objectives Acute myeloid leukemia (AML) is a severe blood cancer with less than 50 % long-term survival. Despite advancements in treatment options, relapse still the major obstacle. The main reason of this problem ineffective targeting leukemic stem cells (LSCs), which play an important role tumor development and relapse. In our previous studies, we found that casticin, polyphenolic component Vitex trifolia ’s fruit, targets both LSCs without affecting healthy tissues. Therefore,...

This study was designed to clarify the effects of D-penicillamine (DPA), a drug used for treatment various pathological events, on lung elastin formation and maturation newborn in perinatal period. The investigation conducted 20 rats bred from 40 female six male rats. DPA doses 400 mg kg−1 day−1 physiological saline were given intraperitoneally (i.p) experimental control groups. To assess maturation, their body weights determined. Serum Cu levels measured by atomic absorption spectroscopy...