A5061718835- Blood properties and coagulation
- Coral and Marine Ecosystems Studies
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Tryptophan and brain disorders
- Protein Degradation and Inhibitors
- Biosimilars and Bioanalytical Methods
- DNA Repair Mechanisms
- Enzyme Production and Characterization
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Advanced Cellulose Research Studies
- Eosinophilic Disorders and Syndromes
- Bacterial Genetics and Biotechnology
- Genetic Neurodegenerative Diseases
- Marine and coastal plant biology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Galectins and Cancer Biology
Northwestern University
2012-2025
Northwestern University
2025
University of Pittsburgh
2000-2013
Erasmus MC
2008
University of Chicago
2008
City University of New York
2000
Hunter College
2000
At the forefront of ecosystems adversely affected by climate change, coral reefs are sensitive to anomalously high temperatures which disassociate (bleaching) photosynthetic symbionts (Symbiodinium) from hosts and cause increasingly frequent severe mass mortality events. Susceptibility bleaching is variable among corals, determined unknown proportions environmental history synergy Symbiodinium- coral-specific properties. Symbiodinium live within host tissues overlaying skeleton, increases...
Indoleamine 2,3-dioxygenase 1 (IDO1) is a potent immunosuppressive enzyme that inhibits the antitumor immune response through both tryptophan metabolism and non-enzymatic functions. To date, most IDO1-targeted approaches have focused on inhibiting metabolism. However, this class of drugs has failed to improve overall survival patients with cancer. Here, we developed characterized proteolysis targeting chimeras (PROTACs) degrade IDO1 protein. IDO1-PROTACs were tested for their effects...
Indoleamine 2,3-dioxygenase 1 (IDO1) is a potently immunosuppressive protein that inhibits antitumor immunity through both tryptophan metabolism and non-enzymatic functions. Pharmacological therapies targeting IDO1 enzyme activity have generally failed to improve the overall survival of patients with cancer. Developing new therapeutic agents are capable neutralizing enzyme-and non-enzyme-derived effects therefore high interest. We previously described development novel Proteolysis Targeting...
Indoleamine 2,3-dioxygenase 1 (IDO1) is an immunosuppressive protein that inhibits antitumor immunity through both tryptophan metabolism and nonenzymatic functions. Drugs targeting IDO1 enzyme activity have failed to improve the overall survival of patients with cancer. Developing new therapeutics neutralize enzyme- nonenzyme-derived effects therefore high interest. We previously described a novel proteolysis chimera (PROTAC), NU223612, degrades in cultured human glioblastoma (GBM) cells, as...
Abstract Proteins that bind preferentially to specific recognition sites on DNA also more weakly nonspecific DNA. We have studied both and non-specific binding of the EcoRI BamHI restriction endonucleases, determined enthalpic entropic contributions free energy (ΔG°bind) using van't Hoff method isothermal titration calorimetry. Specific is characterized by a strongly negative ΔC°p can be either enthalpy-driven or entropy-driven, depending temperature. Nonspecific has ≈ 0 enthalpy-driven. A...
The ability of aptamers to recognize a variety different molecules has fueled their emergence as recognition agents probe complex media and cells. Many detection strategies require aptamer binding its target result in dramatic change structure, typically from an unfolded folded state. Here, we report strategy based on forced intercalation (FIT) that increases the scope by transducing subtle changes structures into fluorescent readouts. By screening library green-fluorescent FIT-aptamers...
Myeloproliferative neoplasms (MPNs) are characterized by the activated JAK2/STAT pathway. Pleckstrin-2 (Plek2) is a downstream target of JAK2/STAT5 pathway and overexpressed in patients with MPNs. We previously revealed that Plek2 plays critical roles pathogenesis JAK2-mutated The nonessential under physiologic conditions make it an ideal for MPN therapy. Here, we identified first-in-class inhibitors through silico high-throughput screening approach cell-based assays, followed synthesis...
Tissue transglutaminase (TG2) is a multifunctional protein with enzymatic, GTP-ase, and scaffold properties. TG2 interacts fibronectin (FN) through its N-terminus domain, stabilizing integrin complexes, which regulate cell adhesion to the matrix. Through this mechanism, participates in key steps involved metastasis ovarian other cancers. High-throughput screening identified several small molecule inhibitors (SMI) for TG2/FN complex. Rational medicinal chemistry optimization of hit compound...
Rad51 is the central catalyst of homologous recombination in eukaryotes and thus critical for maintaining genomic integrity. Recent crystal structures filaments formed by closely related archeal RadA eubacterial RecA proteins place ATPase site at protomeric interface. To test relevance this feature, we mutated conserved residues interface examined their effects on key activities Rad51: ssDNA-stimulated ATP hydrolysis, DNA binding, polymerization substrates catalysis strand-exchange...
Abstract We have previously shown that the Saccharomyces cerevisiae
Abstract Cdt1 is a protein critical for DNA replication licensing and well-established to be binding partner of the minichromosome maintenance (MCM) complex. has also been demonstrated have an emerging, “moonlighting” role at kinetochore via direct microtubules Ndc80 However, it not known how structure conformations could allow these multiple, completely unique sets complexes. And while there exist multiple robust methods study entirely folded or unfolded proteins, structure-function studies...
ABSTRACT Cdt1 is a mixed folded protein critical for DNA replication licensing and it also has “moonlighting” role at the kinetochore via direct binding to microtubules Ndc80 complex. However, unknown how structure conformations of could allow participate in these multiple, unique sets complexes. While robust methods exist study entirely or unfolded proteins, structure–function studies combined, folded/disordered proteins remain challenging. In this work, we employ orthogonal biophysical...
Abstract The ability of aptamers to recognize a variety different molecules has fueled their emergence as recognition agents probe complex media and cells. Many detection strategies require aptamer binding its target result in dramatic change structure, typically from an unfolded folded state. Here, we report strategy based on forced intercalation (FIT) that increases the scope by transducing subtle changes structures into fluorescent readouts. By screening library green‐fluorescent...
Additional file 1: Figure S1. Dynamics of holobiont reflectance (R H ). Panels a–f are aligned into columns defined by light (broken line in a) and temperature (dotted conditions (described S1). Response an exemplar low- $$ \mu ^{\prime}_{{S,m}} μ S , m ′ coral (S. pistillata) through (b) time series photos explants, (c) spectral RH, (f) means (black line) standard errors the 10 random measurements collected to estimate R normalized its skeleton at 675 nm. high- (M. digitata) (d) (e) RH...
<div>Abstract<p>Tissue transglutaminase (TG2) is a multifunctional protein with enzymatic, GTP-ase, and scaffold properties. TG2 interacts fibronectin (FN) through its N-terminus domain, stabilizing integrin complexes, which regulate cell adhesion to the matrix. Through this mechanism, participates in key steps involved metastasis ovarian other cancers. High-throughput screening identified several small molecule inhibitors (SMI) for TG2/FN complex. Rational medicinal chemistry...