A5072969883- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Hepatitis C virus research
- Photosynthetic Processes and Mechanisms
- Advanced Proteomics Techniques and Applications
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Hepatitis B Virus Studies
- Enzyme Structure and Function
- Heavy metals in environment
- RNA modifications and cancer
- EEG and Brain-Computer Interfaces
- Photoreceptor and optogenetics research
- Plant Stress Responses and Tolerance
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Functional Brain Connectivity Studies
- Viral Infections and Immunology Research
- Liver Disease Diagnosis and Treatment
- Advanced Breast Cancer Therapies
- HIV/AIDS drug development and treatment
- Algal biology and biofuel production
- Heavy Metal Exposure and Toxicity
Lahore University of Management Sciences
2018-2025
Kagoshima University
2019
University of Engineering and Technology Lahore
2018
Hepatitis C virus (HCV) is a major public health concern globally, resulting in liver-related complications. Approximately 6% population of Pakistan infected with HCV. HCV error prone, due to which it classified into 7 genotypes and 67 subtypes. genotype determination critical for treatment therapy response. In this study, 3,539 samples were collected from 2015 2019 all over Punjab. RNA was extracted using QIA Amp Viral MINI kit (Qiagen, Germany) viral genotyping performed. Furthermore,...
Hepatitis C is an inflammatory liver disease caused by the single-stranded RNA (ssRNA) hepatitis virus (HCV). The genetic diversity of and quasispecies produced during replication have resulted in viral resistance to direct-acting antivirals (DAAs) as well impediments vaccine development. recent adaptation CRISPR-Cas alternative antiviral approach has demonstrated degradation nucleic acids eukaryotes. In particular, CRISPR-effector Cas13 enzyme been shown target ssRNA viruses effectively....
Computational modeling and analysis of biomolecular network models annotated with omics data are emerging as a versatile tool for designing personalized therapies. Current endeavors aimed at employing in silico towards cancer therapeutics remain limited providing all-in-one approach that ascertains actionable targets, re-positions FDA (Food Drug Administration) approved drugs, furnishes quantitative cues on therapy responses such efficacy cytotoxic effect, identifies novel drug combinations....
Previously, we established a microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) tissues using RNA sequencing and found significantly reduced of miR-204-5p. Here, aimed to investigate the functional significance miR-204-5p identify target genes involved PDAC pathogenesis. Cancer cell migration invasion were inhibited by ectopic cells. Comprehensive gene analyses silico database searches revealed 25 putative targets regulated Among these genes, high levels...
Environment and health hazards posed by the contamination of existing underground water resources are amongst leading anthropogenic challenges in South Asia. One such major environmental hazard is transnational wastewater drain – Hudiara, which degrading quality underlying aquifers. Towards a spatiotemporal assessment Hudiara's drinking from its adjoining areas, total 56 42 samples were obtained over seven months. Analysis data revealed complex interplay geogenic factors HM burden to high...
Abstract Plants employ photosynthesis to produce sugars for supporting their growth. During photosynthesis, an enzyme Ribulose 1,5 bisphosphate carboxylase/oxygenase (Rubisco) combines its substrate (RuBP) with CO 2 phosphoglycerate (PGA). Alongside, Rubisco also takes up O and 2-phosphoglycolate (2-PG), a toxic compound broken down into PGA through photorespiration. Photorespiration is not only resource-demanding process but results in loss which affects photosynthetic efficiency C 3...
Abstract Top-Down Proteomics (TDP) is an emerging proteomics protocol that involves identification, characterization, and quantitation of intact proteins using high-resolution mass spectrometry. TDP has edge over other protocols in it allows for: (i) accurate measurement protein mass, (ii) high sequence coverage, (iii) enhanced identification post-translational modifications (PTMs). However, the complexity spectra poses a significant impediment to search PTM characterization. Furthermore,...
PERCEPTRON is a next-generation freely available web-based proteoform identification and characterization platform for top-down proteomics (TDP). search pipeline brings together algorithms (i) intact protein mass tuning, (ii) de novo sequence tags-based filtering, (iii) of terminal as well post-translational modifications, (iv) truncated proteoforms, (v) in silico spectral comparison, (vi) weight-based candidate scoring. High-throughput performance achieved through the execution optimized...
Schizophrenia (SZ) is a debilitating disorder in which patients exhibit psychotic behavior due to aberrant connectivity between different regions of the brain. Advances neuroimaging have now enabled diagnosis and analysis SZ order elucidate whole brain functional networks. In present study, we used resting-state magnetic resonance imaging (rs-fMRI) causal relationships amongst differentially activated (n=10) healthy controls (n=10). Vector auto-regression (VAR) model Granger causality (GC)...
The Escherichia coli CheY protein belongs to a large bacterial response regulator superfamily. X-ray hydroxy radical foot-printing with mass spectroscopy (XFMS) has shown that allosteric activation of by its motor target triggers concerted internalization aromatic sidechains. We reanalyzed the XFMS data compare polar versus non-polar residue positions. residues around and including 57D phosphorylated site had an elevated reactivity. Bioinformatic measures revealed water-mediated hydrogen...
ABSTRACT Computational modeling and analysis of biomolecular network models annotated with cancer patient-specific multi-omics data can enable the development personalized therapies. Current endeavors aimed at employing in silico towards therapeutics remain to be fully translated. In this work, we present “ CanSeer ” a novel multi-stage methodology for developing clinical translation therapeutics. The proposed integrates state-of-the-art dynamical genomic transcriptomic assess individualized...
Abstract The Escherichia coli CheY protein belongs to a large bacterial response regulator superfamily. X-ray hydroxy radical foot-printing with mass spectroscopy (XFMS) has shown that allosteric activation of by its motor target triggers concerted internalization aromatic sidechains. We reanalyzed the XFMS data compare polar versus non-polar residue positions. residues around and including 57D phosphorylated site had an elevated reactivity. Bioinformatic measures revealed water-mediated...