A5016601963- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Neuroendocrine Tumor Research Advances
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gastrointestinal Tumor Research and Treatment
- Cancer Genomics and Diagnostics
- Hepatocellular Carcinoma Treatment and Prognosis
- Gallbladder and Bile Duct Disorders
- Pancreatitis Pathology and Treatment
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Vascular Malformations and Hemangiomas
- Pediatric Hepatobiliary Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Gastric Cancer Management and Outcomes
- Renal cell carcinoma treatment
- Circular RNAs in diseases
- Cancer Cells and Metastasis
- Oral and Maxillofacial Pathology
- Anorectal Disease Treatments and Outcomes
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Kruppel-like factors research
Kagoshima University
2016-2024
// Tetsuya Idichi 1 , Naohiko Seki 2 Hiroshi Kurahara Keiichi Yonemori Yusaku Osako Takayuki Arai Atsushi Okato Yoshiaki Kita Takaaki Arigami Yuko Mataki Kijima Kosei Maemura and Shoji Natsugoe Department of Digestive Surgery, Breast Thyroid Graduate School Medical Sciences, Kagoshima University, Kagoshima, Japan Functional Genomics, Chiba University Medicine, Chiba, Correspondence to: Seki, email: naoseki@faculty.chiba-u.jp Keywords: microRNA, miR-217 pancreatic ductal adenocarcinoma, ANLN...
Aberrantly expressed microRNA (miRNA) are known to disrupt intracellular RNA networks in cancer cells. Exploring miRNA‐dependent molecular is a major challenge research. In this study, we performed RNA‐sequencing of breast (BrCa) clinical specimens identify tumor‐suppressive miRNA BrCa. total, 64 were identified as candidate BrCa Analysis our signature revealed that several duplexes (guide strand/passenger strand) derived from pre‐miRNA downregulated tissues (e.g. miR‐99a‐5p/‐3p ,...
Analysis of the microRNA (miRNA) expression signature head and neck squamous cell carcinoma (HNSCC) based on RNA sequencing showed that dual strands pre‑miR‑145 (miR‑145‑5p, guide strand; miR‑145‑3p, passenger strand) were significantly reduced in cancer tissues. In miRNA biogenesis, miRNAs are degraded have no biological activities cells. The aims this study to investigate functional significance strand miR‑145 identify miR‑145‑3p‑regulated oncogenic genes HNSCC Expression levels miR‑145‑5p...
Analysis of our original micro RNA (mi ) expression signature patients with advanced renal cell carcinoma ( RCC showed that ‐10a‐5p miR‐10a‐5p was significantly downregulated in specimens. The aims the present study were to investigate antitumor roles and novel cancer networks regulated by this mi cells. Downregulation confirmed tissues from treated tyrosine kinase inhibitors TKI ). Ectopic lines (786‐O A498 cells) inhibited migration invasion. Spindle kinetochore‐associated protein 1 SKA...
Due to its aggressive nature, pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal and hard-to-treat malignancies. Recently developed targeted molecular strategies have contributed remarkable improvements in treatment several cancers. However, such therapies not been applied PDAC. Therefore, new options are needed for PDAC based on current genomic approaches. Expression microRNA-375 (miR-375) was significantly reduced miRNA expression signatures types cancers, including The aim...
// Keiichi Yonemori 1 , Naohiko Seki 2 Tetsuya Idichi Hiroshi Kurahara Yusaku Osako Koshizuka Takayuki Arai Atsushi Okato Yoshiaki Kita Takaaki Arigami Yuko Mataki Kijima Kosei Maemura and Shoji Natsugoe Department of Digestive Surgery, Breast Thyroid Graduate School Medical Sciences, Kagoshima University, Sakuragaoka, 890-8520, Japan Functional Genomics, Chiba University Medicine, Chuo-ku, 260-8670, Correspondence to: Seki, email: naoseki@faculty.chiba-u.jp Keywords: pancreatic ductal...
Solid pseudopapillary neoplasm (SPN) is a rare pancreatic tumor that predominantly affects young females. Prognosis excellent; however, 10-15% of patients show metastasis at the time surgery or develop recurrence after pancreatectomy.We reviewed clinical course three with advanced recurrent SPN and subsequently underwent multidisciplinary treatment our institution between 2002 2019. The primary was resected in all patients, metastases were also if indicated. Intensive combined therapy,...
Analysis of our microRNA (miRNA) expression signature in human cancers has shown that guide and passenger strands pre-miR‑150, i.e., miR‑150‑5p miR‑150‑3p, are significantly downregulated cancer tissues. In miRNA biogenesis, the strand is degraded thought to have no functions. Thus, aim this study was investigate functional significance miR‑150‑3p naïve prostate (PCa) castration-resistant (CRPC). Ectopic assays showed both miRNAs suppressed cell migration invasion. Our strategies target...
// Tetsuya Idichi 1 , Naohiko Seki 2 Hiroshi Kurahara Haruhi Fukuhisa Hiroko Toda Masataka Shimonosono Yasutaka Yamada Takayuki Arai Yoshiaki Kita Yuko Kijima Mataki Kosei Maemura and Shoji Natsugoe Department of Digestive Surgery, Breast Thyroid Graduate School Medical Sciences, Kagoshima University, Kagoshima, Japan Functional Genomics, Chiba University Medicine, Chiba, Correspondence to: Seki, email: naoseki@faculty.chiba-u.jp Keywords: microRNA; miR-124-3p; ITGA3; ITGB1; pancreatic...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive types cancer, and its prognosis abysmal; only 25% patients survive year, 5% live for five years. MicroRNA (miRNA) signature analysis PDAC revealed that both strands pre-miR-30c (miR-30c-5p, guide strand; miR-30c-2-3p, passenger strand) were significantly downregulated, suggesting they function as tumor-suppressors in cells. Ectopic expression assays demonstrated these miRNAs attenuated aggressiveness cells, e.g., cell...
The aggressive nature of intrahepatic cholangiocarcinoma (ICC) renders it a particularly lethal solid tumor. Searching for therapeutic targets ICC is an essential challenge in the development effective treatment strategy. Our previous studies showed that miR-29-3p-family members (miR-29a-3p, miR-29b-3p and miR-29c-3p) are key tumor-suppressive microRNAs control many oncogenic genes/pathways several cancers. In this study, we searched using as starting point. functional cell proliferation,...
We previously used RNA sequencing to establish the micro (mi ) expression signature of pancreatic ductal adenocarcinoma ( PDAC ). found that both strands pre‐ miR‐148a miR‐148a‐5p : passenger strand and miR‐148a‐3p guide strand) were downregulated in cancer tissues. Ectopic significantly inhibited cell migration invasion, indicating had tumor‐suppressive roles cells. In silico database genome‐wide gene analyses identified a total 15 genes putative targets regulated by these miRNAs. High...
Previously, we established a microRNA (miRNA) expression signature in pancreatic ductal adenocarcinoma (PDAC) tissues using RNA sequencing and found significantly reduced of miR-204-5p. Here, aimed to investigate the functional significance miR-204-5p identify target genes involved PDAC pathogenesis. Cancer cell migration invasion were inhibited by ectopic cells. Comprehensive gene analyses silico database searches revealed 25 putative targets regulated Among these genes, high levels...
We recently determined the RNA sequencing-based microRNA (miRNA) expression signature of colorectal cancer (CRC). Analysis showed that both strands pre-miR-139 (miR-139-5p, guide strand, and miR-139-3p, passenger strand) was significantly reduced in CRC tissues. Transient transfection assays revealed miR-139-3p blocked cell malignant transformation (e.g., proliferation, migration, invasion). Notably, markedly RAC-alpha serine/threonine-protein kinase (AKT) phosphorylation cells. A...
Although miR‑145‑5p (the guide strand of the miR‑145 duplex) is established as a tumor suppressive microRNA (miRNA or miR), functional significance miR‑145‑3p passenger in cancer cells and its targets remains obscure. In our continuing analysis esophageal squamous cell carcinoma (ESCC) pathogenesis, aim present study was to identify important oncogenes proteins that are controlled by miR‑145‑3p. Overexpression significantly reduced proliferation, migration invasive abilities, further...
Our recent studies have implicated some passenger strands of miRNAs in the molecular pathogenesis human cancers. Analysis microRNA (miRNA) expression signature pancreatic ductal adenocarcinoma (PDAC) has shown that levels miR-30a-3p, strand derived from pre-mir-30a, are significantly downregulated PDAC tissues. This study aimed to identify oncogenes closely involved under regulation miR-30a-3p. Ectopic assays showed miR-30a-3p inhibited aggressiveness cells, suggesting acts as a...