A5046528198- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Viral Infections and Outbreaks Research
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Herpesvirus Infections and Treatments
- vaccines and immunoinformatics approaches
- Biochemical and Molecular Research
- Virus-based gene therapy research
- Malaria Research and Control
- Extracellular vesicles in disease
- COVID-19 Clinical Research Studies
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Cancer-related Molecular Pathways
- Infectious Diseases and Tuberculosis
- RNA modifications and cancer
Centre of Excellence in Molecular Biology
2016-2025
University of the Punjab
2016-2025
Jena University Hospital
2020
Université Grenoble Alpes
2016
Institut pour l'avancée des biosciences
2016
Higher Education Commission
2016
University Health Network
2010-2015
University of Toronto
2010-2015
Breast Cancer Research Foundation
2014
Princess Margaret Cancer Centre
2009
In cancer, DJ-1/PARK7 acts as an oncogene that drives Akt-mediated cell survival. Although amplification of DJ-1 has been described in several types tumors, the mechanistic basis DJ-1's oncogenic effect remains incompletely understood. A tumor's ability to adapt hypoxia is absolutely critical for its survival and progression, this adaptation largely mediated by transcription factor HIF1. The stabilization HIF1 subunits during at least partly dependent on PI3K/Akt/mTOR pathway. We...
Since the first reported outbreak of dengue hemorrhagic fever in Pakistan, several mini outbreaks have erupted region. Dengue virus serotype 3 (DEN-3) was documented 2005 Karachi. Reports show that is prevalent Lahore since 2008. Serotype 2 (DEN-2) major circulating Pakistan as it 1994. We conducted a detailed study three infection occurred years 2007, 2008 and 2009 by using molecular techniques such PCR nucleotide sequencing C-prM gene junction virus.Through analysis 114 serum samples...
Cytokines are crucial in controlling inflammation during viral infection, particularly infection with the hepatitis C virus (HCV). Cytokine genetic polymorphisms can change how immune system responds to this infection. We investigated HCV treatment was affected by single nucleotide these genes. The goal of study examine any connections between cytokine gene for interleukins (IL)-22-binding protein rs6570136, as well IL-10 rs1800872 and rs1878672 Pakistani population responsiveness...
Nucleic acid amplification assays including PCR have revolutionized the detection of Mycobacterium tuberculosis (MTB). Tuberculosis spread to almost every organ body and is characterized on basis localization infection. Therefore, different types fluids tissues can be used for MTB. From 2004 2010 total 766 smear negative samples from patients, clinically suspected were received investigated at Division Molecular Diagnostics, University Punjab Lahore diagnosis tuberculosis. Mycobacterial DNA...
The highly conserved ANP32 proteins are proposed to function in a broad array of physiological activities through molecular mechanisms as diverse phosphatase inhibition, chromatin regulation, caspase activation, and intracellular transport. On the basis previous analyses mice bearing targeted mutations Anp32a or Anp32e , there has been speculation that all play redundant roles dispensable for normal development. However, more recent work suggested ANP32B may fact have functions not shared by...
Abstract Background & Aims The pro‐inflammatory cytokine IL ‐17 plays a crucial role in liver diseases associated with hepatic fibrosis and increased risk of cancer development. Nevertheless, the cellular source this has never been characterized patients fibrosis. Methods In study, we investigated biopsies from 49 chronic viral hepatitis at different stages We monitored production by intracellular flow cytometry, immunofluorescence immunohistochemical situ stainings, allowing precise...
Hepatitis C is an inflammatory liver disease caused by the single-stranded RNA (ssRNA) hepatitis virus (HCV). The genetic diversity of and quasispecies produced during replication have resulted in viral resistance to direct-acting antivirals (DAAs) as well impediments vaccine development. recent adaptation CRISPR-Cas alternative antiviral approach has demonstrated degradation nucleic acids eukaryotes. In particular, CRISPR-effector Cas13 enzyme been shown target ssRNA viruses effectively....
Hepatitis C virus (HCV) is one of the leading causes viral hepatitis worldwide and its genotype 3a predominant in vast areas Pakistan.The present study reports first full sequence HCV isolate PK-1 from Pakistan. This nucleotide was compared with six other length sequences different regions world by using statistical methods phylogenetic analysis.The difference these seven shows that phylogenetically distinct origin circulating
Background Accumulated literature suggests that the acidic nuclear phosphoprotein 32 kilodalton (Anp32) proteins control multiple cellular activities through different molecular mechanisms. Like other Anp32 family members, Anp32e (a.k.a. Cpd1, PhapIII) has been conserved throughout vertebrate evolution, suggesting it an important function in organismal survival. Principal Findings Here, we demonstrate gene can be deleted mice without any apparent effect on their wellbeing. No defects...
Introduction: Dengue virus (DENV) is an arthropod-borne that belonged to the Flaviviridae viral family.Four known serotypes DEN-1 through DEN-4 do exist and circulate in diverse geographical regions of world causing epidemics.The management dengue patients, especially hemorrhagic fever (DHF)/Dengue shock syndrome (DSS) cases, has been a challenge Pakistan now days.Method: We have carried out comprehensive study current outbreaks infection on molecular level with aim find common serotype/s...
Significance T-cell homeostasis is a tightly regulated process that ensures constant number of naïve T cells in the periphery an organism. Mechanisms promote survival and homeostatic proliferation peripheral are essential for maintaining this balance thus effective immunity against pathogens incipient tumors. We have identified UV radiation resistance-associated gene (UVRAG), autophagic tumor suppressor many cell lineages, as novel nonautophagic regulator homeostasis. Furthermore, our...