A5010902238- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- RNA Research and Splicing
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Virus-based gene therapy research
- Neuroscience of respiration and sleep
- Developmental Biology and Gene Regulation
- Sperm and Testicular Function
- Infective Endocarditis Diagnosis and Management
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic Neurodegenerative Diseases
- Xenotransplantation and immune response
- Renal and related cancers
- Congenital heart defects research
- Animal Virus Infections Studies
- Genetics and Neurodevelopmental Disorders
- Antimicrobial Resistance in Staphylococcus
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
Northwest A&F University
2007-2024
South China Agricultural University
2024
Westlake University
2023-2024
Children's Research Hospital
2022
Despite pluripotent stem cells sharing key transcription factors, their maintenance involves distinct genetic inputs. Emerging evidence suggests that super-enhancers (SEs) can function as master regulatory hubs to control cell identity and pluripotency in humans mice. However, whether pluripotency-associated SEs share an evolutionary origin mammals remains elusive. Here, we performed comprehensive comparative epigenomic factor binding analyses among pigs, humans, mice identify SEs. Like...
The pluripotency gene regulatory network of porcine induced pluripotent stem cells(piPSCs), especially in epigenetics, remains elusive. To determine the biological function we cultured piPSCs different culture conditions. We found that activation gene- and pluripotency-related pathways requires erasure H3K9 methylation modification which was further influenced by mouse embryonic fibroblast (MEF) served feeder. By dissecting dynamic change during loss pluripotency, demonstrated demethylases...
LIN28A, an RNA-binding protein, plays important role in porcine induced pluripotent stem cells (piPSCs). However, the molecular mechanism underlying function of <i>LIN28A</i> maintenance pluripotency piPSCs remains unclear. Here, we explored based on its overexpression and knockdown. We performed total RNA sequencing (RNA-seq) detected expression levels relevant genes by quantitative real-time polymerase chain reaction (qRT-PCR), western blot analysis, immunofluorescence staining. Results...
To date, many efforts have been made to establish porcine embryonic stem (pES) cells without success. Extraembryonic endoderm (XEN) can self-renew and differentiate into the visceral parietal endoderm. XEN are derived from primitive of inner cell mass blastocysts may be an intermediate state in reprogramming.Porcine (pXENCs) were generated pluripotent (pPSCs) characterized by RNA sequencing immunofluorescence analyses. The developmental potential pXENCs was investigated chimeric mouse...
Abstract Objectives The establishment of porcine pluripotent stem cells (pPSCs) is still a critical topic. However, all pPSCs were failed to contribute efficient chimeric pig and extremely sensitive changes culture conditions. This study aimed investigate the role BCL2 in further explain mechanism. Materials Methods Porcine gene was cloned overexpressed induce (piPSCs). Digital RNA‐seq performed mechanism anti‐apoptosis. Finally, carrying injected into mouse early embryo evaluate its...
Pluripotent stem cells (PSCs) are useful for developmental and translational research because they have the potential to differentiate into all cell types of an adult individual. Pigs one most important domestic ungulates, commonly used food as bioreactors. Generating stable pluripotent porcine PSC lines remains challenging. So far, pluripotency gene network PSCs is poorly understood. Here we found that TBX3-derived induced (iPSCs) closely resemble 4-cell embryos with capacity...
Liquid-liquid phase separation (LLPS) mediated by G3BP1/2 proteins and non-translating mRNAs mediates stress granule (SG) assembly. We investigated the phylogenetic evolution of G3BP orthologs from unicellular yeast to mammals identified both conserved divergent features. The modular domain organization is generally conserved. However, invertebrate displayed reduced capacity for SG assembly in human cells compared vertebrate orthologs. demonstrated that protein-interaction network...
Porcine-induced pluripotent stem cells (piPSCs) could serve as a great model system for human cell preclinical research. However, the pluripotency gene network of piPSCs, especially function core transcription factor estrogen-related receptor beta (ESRRB), was poorly understood. Here, we constructed ESRRB-overexpressing piPSCs (ESRRB-piPSCs). Compared with control (CON-piPSCs), ESRRB-piPSCs showed flat, monolayered colony morphology. Moreover, greater chimeric capacity into trophectoderm...
The establishment of porcine pluripotent stem cells (piPSCs) is critical but remains challenging. All piPSCs are extremely sensitive to minor perturbations culture conditions and signaling network. Inhibitors, such as CHIR99021 XAV939 targeting the WNT pathway, have been added in a medium modify cell regulatory However, potential side effects inhibitors could confine pluripotency practicability piPSCs. This study aimed investigate roles AXIN, one component pathway Here, AXIN1 AXIN2 genes...
A renewable source of porcine macrophages derived from pluripotent stem cells (PSCs) would be a valuable alternative to primary alveolar (PAMs) in the research host-pathogen interaction mechanisms. We developed an efficient and rapid protocol, within 11 days, derive PSCs (pPSCs). The pPSC-derived (pPSCdMs) exhibited molecular functional characteristics macrophages. pPSCdMs showed macrophage-specific surface protein expression transcription factors, similar PAMs. also macrophages, such as...
Spermatogenesis is an important physiological process associated with male infertility. As a kind of post-transcriptional regulation, RNA editings (REs) change the genetic information at mRNA level. But whether there are REs and what's role during still unclear. In this study, we integrated published RNA-Seq datasets established landscape development mouse spermatogenesis. Totally, 7530 editing sites occurred in 2012 genes among all types germ cells were found, these enrich on some regions...
Abstract The pluripotency gene regulatory network of porcine-induced pluripotent stem cells (piPSCs), especially in epigenetics, remains elusive. To determine this biological function we cultured piPSCs different culture conditions. We found that activation gene- and pluripotency-related pathways requires the erasure H3K9 methylation modification which was further influenced by mouse embryonic fibroblast (MEF) served feeder. By dissecting dynamic change during loss pluripotency, demonstrated...
Abstract Despite pluripotent stem cells sharing key transcription factors, their maintenance involves distinct genetic inputs. Emerging evidence suggests that super-enhancers (SEs) can function as master regulatory hubs to control cell identity and pluripotency in humans mice. However, whether pluripotency-associated SEs share a deep evolutionary origin mammals remains elusive. Here, we performed comprehensive comparative epigenomic factor binding analyses among pigs, humans, mice identify...
Abstract Porcine embryonic fibroblasts (PEFs) can be directly reprogrammed into porcine induced pluripotent stem cells (piPSCs). However, the reprogramming process is generally lengthy and inefficient. Here, we established a fast efficient induction system of piPSCs from Sertoli (SCs) via forced expression pig Yamanaka factors. The alkaline phosphatase (AP)‐positive colonies SCs developed on Day 3 after lentivirus infection, were expanded then picked up 7, whereas PEFs did not show any in...
As main recipient cells for porcine reproductive and respiratory syndrome virus (PRRSV), alveolar macrophage (PAM) are involved in the progress of several highly pathogenic infections. However, due to fact that PAM can only be obtained from primary tissues, research on PAM-based virus-host interactions remains challenging. The improvement induced pluripotent stem (iPSCs) technology provides a new strategy develop IPSCs-derived cells. Since CD163 is macrophage-specific marker validated...
Abstract. The hypocretin receptor1 (HCRTR1) gene encodes a Orexin A receptor (SAKURAI et al., 1998). It belongs to the class Ι subfamily within superfamily of G-coupled receptors and is coupled Ca2+ mobilization. Via HCRTR1, involved in control feeding, sleep-wakefulness, neuroendocrine homeostasis autonomic regulation (VOISIN 2003; TAKESHI SAKURAI, 1999). These characters are important animal production. In human, 408 isoleucine valine mutation HCRTR1 showed significant association with...
ABSTRACT Spermatogenesis is an important physiological process associated with male infertility. But whether there are RNA editings (REs) and what’s the role of REs during still unclear. In this study, we integrated published RNA-Seq datasets established a landscape development mouse spermatogenesis. 7530 editing sites among all types germ cells were found, which enrich on some regions chromosome, including chromosome 17 both ends Y. Totally, occur in 2012 genes spermatogenesis, more than...
Abstract BackgroundThe establishment of porcine pluripotent stem cells (piPSCs) is still a critical topic and challenging issue. However, all piPSCs are extremely sensitive to changes the culture conditions.In addition, side effect inhibitors in medium confine pluripotency practicability. This study aimed investigate roles AXIN further explore mechanism. Here, AXIN1 gene AXIN2 were knockdown, cloned, overexpressed piPSCs. Digital RNA-seq was performed mechanism cell proliferation...
Porcine spermatogonia stem cells (pSSCs) are the only type of somatic cell that can pass genetic information to successive generations. Little is known about pSSCs vitality in vitro, and due their increasing importance research, here, we optimized a protocol culture explored potential fate vitro. Utilizing feeder-free system with 2D peptide-coating small chemical molecules (including CHIR99021, Repsox, vitamin C, folic acid, CD lipid concentrate), were able prolong time by at least three...