Triantafillos Liloglou
A5085910405- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- Head and Neck Cancer Studies
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Diagnosis and Treatment
- Pancreatitis Pathology and Treatment
- Hemoglobin structure and function
- Genomic variations and chromosomal abnormalities
- Genetics and Neurodevelopmental Disorders
- Cancer, Hypoxia, and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- Genomics and Chromatin Dynamics
- Genetic Associations and Epidemiology
- MicroRNA in disease regulation
- Salivary Gland Tumors Diagnosis and Treatment
- DNA Repair Mechanisms
- Oral Health Pathology and Treatment
- Porphyrin Metabolism and Disorders
- Histone Deacetylase Inhibitors Research
University of Liverpool
2015-2024
Edge Hill University
2022-2024
Roy Castle Lung Cancer Foundation
2010-2022
John Wiley & Sons (United States)
2017
Columbia Medical Practice
2017
Cancer Research UK
2012-2013
Institut Català d'Oncologia
2013
Hospital de Sant Pau
2013
Institució Catalana de Recerca i Estudis Avançats
2013
Institut d'Investigació Biomédica de Bellvitge
2013
Methylation is one of the major epigenetic processes pivotal to our understanding carcinogenesis. It now widely accepted that there a relationship between DNA methylation, chromatin structure, and human malignancies. methylation potentially an important clinical marker in cancer molecular diagnostics. Understanding modifications their biological context involves several aspects analysis. These include de novo discovery differentially methylated genes, analysis patterns, determination...
Using a model-based approach, we estimated the probability that an individual, with specified combination of risk factors, would develop lung cancer within 5-year period. Data from 579 cases and 1157 age- sex-matched population-based controls were available for this analysis. Significant factors fitted into multivariate conditional logistic regression models. The final model was combined age-standardised incidence data to calculate absolute estimates. Combinations lifestyle modelled create...
Abstract Purpose: Human papillomavirus-16 (HPV16) is the causative agent in a biologically distinct subset of oropharyngeal squamous cell carcinoma (OPSCC) with highly favorable prognosis. In clinical trials, HPV16 status an essential inclusion or stratification parameter, highlighting importance accurate testing. Experimental Design: Fixed and fresh-frozen tissue from 108 OPSCC cases were subject to eight possible assay/assay combinations: p16 immunohistochemistry (p16 IHC); situ...
Genome-wide association studies (GWAS) have been successful in identifying common genetic variation involved susceptibility to etiologically complex disease. We conducted a GWAS identify upper aero-digestive tract (UADT) cancers. genotyping was carried out using the Illumina HumanHap300 beadchips 2,091 UADT cancer cases and 3,513 controls from two large European multi-centre studies, as well 4,821 generic controls. The 19 top-ranked variants were investigated further an additional 6,514...
Epigenetic silencing of O(6)-methylguanine-DNA-methyltransferase (MGMT) by promoter methylation is associated with improved survival in glioblastomas treated alkylating agents. In this study, we investigated MGMT temozolomide and radiotherapy a single UK treatment centre.Quantitative data at individual CpG sites were obtained pyrosequencing for 109 glioblastomas.Median overall (OS) was 12.4 months 2-year 17.9%. Pyrosequencing reproducible archival samples yielding all glioblastomas....
Non-small-cell lung cancer (NSCLC) is a tumor in which only small improvements clinical outcome have been achieved. The issue critical for stage I patients whom there are no available biomarkers that indicate high-risk should receive adjuvant chemotherapy. We aimed to find DNA methylation markers could be helpful this regard.A microarray analyzes 450,000 CpG sites was used study tumoral obtained from 444 with NSCLC included 237 tumors. prognostic were validated by single-methylation...
Individual plasma proteins have been identified as minimally invasive biomarkers for lung cancer diagnosis with potential utility in early detection. Plasma proteomes provide insight into contributing biological factors; we investigated their future prediction.The Olink® Explore-3072 platform quantitated 2941 496 Liverpool Lung Project samples, including 131 cases taken 1-10 years prior to diagnosis, 237 controls, and 90 subjects at multiple times. 1112 significantly associated haemolysis...
Abstract LINE‐1 and Alu elements are non‐LTR retrotransposons, constituting together over 30% of the human genome they frequently hypomethylated in tumors. A relationship between global hypomethylation genomic instability has been shown, however, there is little evidence to suggest active role for hypomethylation‐mediated reactivation retroelements cancer. In our study, we examined by Pyrosequencing methylation levels sequences 48 primary nonsmall cell carcinomas their paired adjacent...
Methylation profiling of cancer tissues has identified this mechanism as an important component carcinogenesis. Epigenetic silencing tumour suppressor genes through promoter methylation been investigated by a variety means, the most recent which is pyrosequencing. We have quantitative status in oral squamous cell carcinoma patients. Fresh tissue and normal control from resection margin was obtained 79 consecutive patients undergoing carcinoma. DNA extracted bisulphite treated. PCR primers...
Abstract Background This study aimed to show that SHOX2 DNA methylation is a tumor marker in patients with suspected lung cancer by using bronchial fluid aspirated during bronchoscopy. Such biomarker would be clinically valuable, especially when, following the first bronchoscopy, final diagnosis cannot established histology or cytology. A test low false positive rate can reduce need for further invasive and costly procedures ensure early treatment. Methods Marker discovery was carried out...
Abstract Introduction Identification of gene expression-based breast cancer subtypes is considered a critical means prognostication. Genetic mutations along with epigenetic alterations contribute to gene-expression changes occurring in cancer. So far, these contributions sporadic have not been well characterized, and only limited understanding exists the mechanisms affected those particular subtypes. The present study was undertaken dissect methylome deliver specific epigenotypes associated...
Human papillomavirus (HPV) testing in oropharyngeal squamous cell carcinoma (OPSCC) is now advocated. Demonstration of transcriptionally active high-risk HPV (HR-HPV) fresh tumour tissue considered to be the analytical 'gold standard'. Clinical has focused on formalin-fixed paraffin-embedded (FFPE) at expense sensitivity and specificity. Recently, a novel RNA situ hybridisation test (RNAscope) been developed for detection HR-HPV FFPE tissue; however, validation against standard' not...
Background Genetic variants in 15q25 have been identified as potential risk markers for lung cancer (LC), but controversy exists to whether this is a direct association, or the 15q variant simply proxy increased exposure tobacco carcinogens. Methods We performed detailed analysis of one single nucleotide polymorphism (SNP) (rs16969968) with smoking behaviour and total 17 300 subjects from five LC studies four upper aerodigestive tract (UADT) studies. Results Subjects minor allele smoked on...
While the association between exposure to secondhand smoke and lung cancer risk is well established, few studies with sufficient power have examined by histological type. In this study, we evaluated smoke-lung relationship type based on pooled data from 18 case–control in International Lung Cancer Consortium (ILCCO), including 2,504 cases 7,276 control who were never smokers 10,184 7,176 controls ever smokers. We used multivariable logistic regression, adjusting for age, sex, race/ethnicity,...
Abstract The exceptional high mortality of lung cancer can be instigated to a degree by late diagnosis. Despite the plethora studies on potential molecular biomarkers for diagnosis, very few have reached clinical implementation. In this study, we developed panel DNA methylation and validated their diagnostic efficiency in bronchial washings from large retrospective cohort. Candidate targets previous high-throughput approaches were examined pyrosequencing an independent set 48 tumor/normal...
Abstract Quantifying the genetic correlation between cancers can provide important insights into mechanisms driving cancer etiology. Using genome-wide association study summary statistics across six types based on a total of 296,215 cases and 301,319 controls European ancestry, here we estimate pair-wise correlations breast, colorectal, head/neck, lung, ovary prostate cancer, 38 other diseases. We observed statistically significant lung head/neck ( r g = 0.57, p 4.6 × 10 −8 ), breast ovarian...
Long non-coding RNAs compose an important level of epigenetic regulation in normal physiology and disease. Despite the plethora publications lncRNAs human cancer, landscape is still unclear.Microarray analysis 44 NSCLC paired specimens was followed by qPCR-based validation 29 (technical) 38 (independent) tissue pairs. Cross-validation selected targets achieved 850 tumours from TCGA datasets.Twelve were successfully validated qPCR (upregulated: FEZF1-AS1, LINC01214, LINC00673, PCAT6,...
Certain members of the ADP-ribosyltransferase superfamily (ARTD or PARP enzymes) catalyse ADP-ribosylation in response to cellular stress, DNA damage and viral infection are upregulated various tumours tolerate increased stress. PARP9, its binding partner DTX3L PARP14 protein levels significantly correlated head neck squamous cell carcinoma (HNSCC) other tumour types though a mechanism where PARP9/DTX3L regulates post-transcriptionally. Depleting expression HNSCC HeLa lines decreases...