A5000954369- Vector-borne infectious diseases
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Yersinia bacterium, plague, ectoparasites research
- Insect and Pesticide Research
- Dermatological diseases and infestations
- Leptospirosis research and findings
- Toxin Mechanisms and Immunotoxins
- Zoonotic diseases and public health
- Herpesvirus Infections and Treatments
- Colorectal Cancer Surgical Treatments
- Bartonella species infections research
- Plant and Fungal Interactions Research
- Parasite Biology and Host Interactions
- Plant Parasitism and Resistance
- Antifungal resistance and susceptibility
- Bird parasitology and diseases
- Antimicrobial Resistance in Staphylococcus
- Facial Nerve Paralysis Treatment and Research
- Streptococcal Infections and Treatments
- Bacillus and Francisella bacterial research
- Colorectal and Anal Carcinomas
- Fungal Infections and Studies
National Institute of Infectious Diseases
2015-2024
Aichi Cancer Center
2022-2023
University of Shizuoka
1992-2022
Peter Doherty Institute
2022
The University of Melbourne
2022
Japanese Red Cross Society Wakayama Medical Center
2022
Ise Red Cross Hospital
2022
Shizuoka Institute of Science and Technology
2022
Gifu University
2009-2018
Jeju National University
2018
Abstract Genetic characteristics of 12 Borrelia isolates from the tick, Ixodes ovatus, I. persulcatus , and rodent, Apodemus speciosus ainu in Japan were compared to members three genospecies burgdorferi sensu lato; B. stricto, garinii group VS461. The methods used this study quantitative microplate DNA hybridization assay restriction fragment length polymorphism (RFLP) analyses flagellin structural genes 16S rRNA genes. six A. identified as using RFLP analysis In contrast, ovatus indicated...
We confirmed infection of 2 patients with Borrelia miyamotoi in Japan by retrospective surveillance Lyme disease and detection B. DNA serum samples. One patient also showed seroconversion for antibody against recombinant glycerophosphodiester phosphodiesterase miyamotoi. Indigenous relapsing fever should be considered a health concern Japan.
We constructed highly transformable and infectious Borrelia burgdorferi B31 by inactivating BBE02, a putative restriction-modification gene on the linear plasmid lp25. The low-passage-number clones 5A4 (containing all plasmids) 5A18 (lp28-4(-) lp56(-)) were used for this study, BBE02 was disrupted homologous recombination. transformation efficiency with shuttle vector pBSV2C03::gntDeltakan increased from <1 to approximately 10 colonies per mug of DNA BBE02::Kan(r) 14 600 BBE02::Kan(r). lp25,...
Summary The members of the genus Borrelia are transmitted by arthropods and known to be infectious vertebrates. Here we found isolates DNAs belonging turcica unknown species from imported reptiles their ectoparasites. strains were isolated blood multiple organs exotic tortoises, experimentally captive‐bred tortoises. These findings suggest that these tortoises may a candidate as reservoir host species. In this study, also and/or detected in hard‐bodied ticks, Amblyomma ticks Hyalomma ticks....
During 2012–2013, a total of 4325 host-seeking adult ticks belonging to the genus Ixodes were collected from various localities Hokkaido, northernmost island Japan. Tick lysates subjected real-time PCR assay detect borrelial infection. The was designed for specific detection Relapsing fever spirochete Borrelia miyamotoi and unspecific Lyme disease-related spirochetes. Overall prevalence B. 2% (71/3532) in persulcatus, 4.3% (5/117) pavlovskyi 0.1% (1/676) ovatus. I. persulcatus significantly...
One of the major routes transmission rickettsial and ehrlichial diseases is via ticks that infest numerous host species, including humans. Besides mammals, reptiles amphibians also carry may harbor Rickettsia Ehrlichia strains are pathogenic to Furthermore, exempt from quarantine in Japan, thus facilitating entry parasites pathogens country through import. Accordingly, current study, we examined presence spp. genes associated with originating outside Japan. Ninety-three representing nine...
Background: Reactivation of herpes simplex virus type 1 is thought to be a major cause adult idiopathic peripheral facial paralysis or Bell's palsy. However, few studies have examined the pathogenesis this condition in children. Serologic assays and polymerase chain reaction (PCR) analysis paired sera saliva samples were used here investigate causes acute pediatric patients. Methods: A total 30 children with recruited. Paired assayed for evidence herpesvirus, mumps Borrelia infection. PCR...
Varicella-zoster virus (VZV) and herpes simplex (HSV) are considered to be the major causes of acute peripheral facial palsy (APFP). One hundred forty-two patients with APFP were analyzed by serological assays polymerase chain reaction analysis. Ramsay Hunt syndrome was diagnosed in 21 patients. Of remaining 121 clinically Bell's palsy, VZV reactivation without zoster (zoster sine herpete) detected 35 (29%). The prevalence antibodies HSV among significantly higher than those (Ramsay or...
ABSTRACT The presence of the linear plasmids lp25 and lp56 Borrelia burgdorferi B31 was found to dramatically decrease rate transformation by electroporation with shuttle vector pBSV2, an autonomously replicating plasmid that confers kanamycin resistance (P. E. Stewart, R. Thalken, J. L. Bono, P. Rosa, Mol. Microbiol. 39:714-721, 2001). B. clones had efficiencies were either low, intermediate, or high, this phenotype correlated absence lp56. Under conditions utilized in study, no...
Previously, a novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium, which infests tortoises (Testudo graeca), by using Barbour–Stoenner–Kelly (BSK) II medium; samples were taken Istanbul area in northwestern Turkey [ Güner et al. (2003) . Microbiology 149, 2539–2544]. Here is presented detailed characterization of spirochaete. Electron microscopy revealed that strain IST7T morphologically similar to other spirochaetes genus Borrelia and possesses 15 16 flagellae...
Thirteen patients with leptospirosis were identified, as confirmed by laboratory analysis during the last 5 years in our laboratory, who came from urban areas of Tokyo, Japan. All into contact rats before onset illness. Seventeen per cent Norway captured inner cities Tokyo carried leptospires their kidneys. Most these rat isolates Leptospira interrogans serovar Copenhageni/Icterohaemorrhagiae. Antibodies against serovars and DNA detected patients. This suggests that are important reservoirs...
Here, we describe for the first time prevalence and genetic properties of Bartonella organisms in wild rodents Japan. We captured 685 throughout Japan (in 12 prefectures) successfully isolated from 176 (isolation rate, 25.7%). Those isolates were all obtained suburban areas (rate, 51.8%), but no organism was animals city areas. Sequence analysis rpoB gltA revealed that classified into eight groups, comprising closely related to B. grahamii (A-I group), tribocorum elizabethae (B-J...
The genus Borrelia is arthropod-borne infectious agents in vertebrates, and classified into Lyme disease (LD) spp. Relapsing fever (RF) In addition to these groups, we recently reported reptile-associated (REP) from reptiles hard-bodied ticks, which probably transmitted the REP this study, investigated presence of sp. moulted found that trans-stadial transmission occurred midgut, while it was observed entered salivary gland during blood-feeding. This characteristic also LD spp., are by...
Group A streptococcus (GAS) causes variety of diseases ranging from common pharyngitis to life-threatening severe invasive diseases, including necrotizing fasciitis and streptococcal toxic shock-like syndrome. The characteristic GAS infections has been thought attribute genetic changes in bacteria, however, no clear evidence shown due lack an intriguingly study using serotype-matched isolates clinical infections. In addition, rare outbreaks their distinctive pathology which infectious foci...
To clarify how Borrelia miyamotoi is maintained in the environment Hokkaido, we examined Ixodes persulcatus for its prevalence among wild rodents and tick vector by detecting a portion of borrelial flaB gene rodent urinary bladder blood samples, from whole ticks. We compared B. infection rates to garinii afzelii, which are human Lyme disease pathogens also carried rodents, transmitted same tick. Whereas afzelii showed age dependence (18.4% 9.9% adults 6.0% 3.4% sub-adults, respectively) when...
ABSTRACT Multilocus sequence typing of Borrelia garinii isolates from humans and comparison with rodent tick were performed. Fifty-nine divided into two phylogenetic groups, an association was detected between clinical isolates, suggesting that, in Japan, human-pathogenic B. comes rodents via ticks.
Tick identification is important in control of tick-born diseases because tick-borne pathogens are often transmitted by specific tick species. In this study, we determined partial DNA sequences the mitochondrial 16S rDNA gene (mt-rrs) for ticks including 7 genera and 39 species, these were allocated to 113 sequence types. Of species ticks, 36 (92.3%) distinguishable phylogenetic analysis mt-rrs. This result suggests that based on mt-rrs a viable alternative morphological identification....
Twenty-nine isolates of Lyme borreliosis (LB) group spirochaetes collected from ticks and rodents in China Japan were included a multilocus sequence analysis (MLSA). Using different typing system, three these strains had previously been identified as being divergent other LB spirochaete species the name 'Borrelia yangtze' sp. nov. was proposed. The data presented here confirm that genetic distance, calculated using sequences MLSA housekeeping genes, to known < 95 % Borrelia valaisiana 96.67...
Abstract We retrospectively confirmed 2 cases of human Anaplasma phagocytophilum infection. Patient blood samples contained unique p44/msp2 for the pathogen, and antibodies bound to A. antigens propagated in THP-1 rather than HL60 cells. Unless both cell lines are used serodiagnosis rickettsiosis-like infections, granulocytic anaplasmosis could go undetected.
Relapsing fever is an infectious disease previously neglected in Africa, which imposes a large public health burden the country. We aimed to investigate and report on case of relapsing borreliosis Zambia. A unknown Borrelia species was isolated from blood febrile patient. Investigations presumptive vector ticks natural hosts for were conducted by culture isolation and/or DNA detection Borrelia-specific polymerase chain reaction. Using isolates patient bat specimens, genetic characterization...
Abstract For establishment of a rapid‐identification method Leptospira species, aflaB gene was investigated and the following results were obtained. 1) Hae III‐ or Hin dIII‐restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR) products (793 bp) flaB effectual for classification species . 2) Twenty cells in 1 ml coagulated blood 100 anti‐coagulated could be detected by ‐PCR. These suggested that PCR‐RFLP based on an efficient tool rapid detection identification...