A5086190158- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Ion channel regulation and function
- Neural dynamics and brain function
- Alzheimer's disease research and treatments
- Neuroendocrine regulation and behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Epilepsy research and treatment
- Photoreceptor and optogenetics research
- Primate Behavior and Ecology
- Neuropeptides and Animal Physiology
- Connexins and lens biology
- Cardiac electrophysiology and arrhythmias
- Computational Drug Discovery Methods
- Intraocular Surgery and Lenses
- Anesthesia and Neurotoxicity Research
- Receptor Mechanisms and Signaling
- Glaucoma and retinal disorders
- Nicotinic Acetylcholine Receptors Study
- Facial Trauma and Fracture Management
- Traumatic Brain Injury and Neurovascular Disturbances
- Stress Responses and Cortisol
- Sleep and Wakefulness Research
- Prion Diseases and Protein Misfolding
- Cell Image Analysis Techniques
Columbia University Irving Medical Center
2022-2024
Columbia University
2018-2024
Rush University Medical Center
2013-2021
University of Iowa
2015
Recent results suggest that social memory requires the dorsal hippocampal CA2 region as well a subset of ventral CA1 neurons. However, it is unclear whether and represent parallel or sequential circuits. Moreover, because evidence implicating in comes largely from long-term inactivation experiments, dynamic role remains unclear. Here, we use pharmacogenetics optogenetics mice to acutely reversibly silence its projections hippocampus. We show activity critical for encoding, consolidation,...
Aging-related impairments in hippocampus-dependent cognition have been attributed to maladaptive changes the functional properties of pyramidal neurons within hippocampal subregions. Much evidence has come from work on CA1 neurons, with CA3 receiving comparatively less attention despite its age-related hyperactivation being postulated interfere spatial processing circuit. Here, we use whole-cell current-clamp demonstrate that aged rat (29–32 months) fire significantly more action potentials...
The rodent hippocampus can be divided into dorsal (DHC) and ventral (VHC) domains on the basis of behavioral, anatomical, biochemical differences. Recently, we reported that CA1 pyramidal neurons from VHC were intrinsically more excitable than DHC neurons, but specific ionic conductances contributing to this difference not determined. Here investigated hyperpolarization-activated current (I(h)) expression HCN1 HCN2 channel subunits in VHC. Measurement Ih with cell-attached patches revealed a...
Significance A longstanding question in the field of neurobiology learning and memory is why some aged individuals perform comparably to young adults tasks, whereas others show impairments that resemble performance those with hippocampal damage. Answers this have been critical for advancing our understanding of, ability treat, aging-related cognitive impairments. Here, we exploited exquisite organization circuit reveal “unsuccessful” aging associated a breakdown adult-like organization,...
The impact of a given neuronal pathway depends on the number synapses it makes with its postsynaptic target, strength each individual synapse, and integrative properties dendrites. Here we explore cellular synaptic mechanisms responsible for differential excitatory drive from entorhinal cortical onto mouse CA2 compared CA1 pyramidal neurons (PNs). Although both types receive direct input cortex their distal dendrites, these inputs produce 5- to 6-fold larger EPSP at soma PNs, which is...
De novo mutations in voltage- and ligand-gated channels have been associated with an increasing number of cases developmental epileptic encephalopathies, which often fail to respond classic antiseizure medications. Here, we examine two knock-in mouse models replicating de sequence variations the human HCN1 voltage-gated channel gene, p.G391D p.M153I (Hcn1G380D/+ Hcn1M142I/+ mouse), severe drug-resistant neonatal- childhood-onset epilepsy, respectively. Heterozygous mice from both lines...
Entorhinal cortex neurons make monosynaptic connections onto distal apical dendrites of CA1 and CA2 pyramidal through the perforant path (PP) projection. Previous studies show that differences in dendritic properties synaptic input density enable PP inputs to produce a much stronger excitation compared with neurons. Here, using mice both sexes, we report difference efficacy varies substantially as function presynaptic firing rate. Although single stimulus evokes 5- 6-fold greater EPSP CA1,...
Hyperpolarization-activated, cyclic nucleotide–gated (HCN) channels generate the cationic I h current in neurons and regulate excitability of neuronal networks. The function HCN depends, part, on their subcellular localization. Of four isoforms (HCN1-4), HCN1 is strongly expressed dendrites pyramidal (PNs) hippocampal area CA1 but also presynaptic terminals parvalbumin-positive interneurons (PV+ INs), which provide strong inhibitory control over activity. Yet, little known about how these...
Reversible inactivation was used to examine the conditioned stimulus (CS) pathway for visual eyeblink conditioning (EBC). Previous research has shown that ventral lateral geniculate (LGNv) and nucleus of optic tract (NOT) could play a role in EBC through ipsilateral projections medial pontine nuclei. Rats were given followed by (LGNv), (NOT), or both nuclei contralateral eye. Muscimol infusions into NOT LGNv impaired retention. Combined LGNv/NOT produced most severe impairment. these after...
The present experiment monitored bilateral eyelid responses during eyeblink conditioning in rats trained with a unilateral unconditioned stimulus (US). Three groups of were used to determine if cross-modal savings occurs when the location US is switched from one eye other. Rats each group first received paired or unpaired conditioned (tone light; conditional [CS]) and periorbital electrical stimulation US. All subsequently given training, but (Group 1), CS modality 2), 3) changed. Changing...
Abstract De novo mutations in voltage- and ligand-gated channels have been associated with an increasing number of cases developmental epileptic encephalopathies, which often fail to respond classic antiseizure medications. Here, we examine two knock-in mouse models replicating de the HCN1 voltage-gated channel gene, p.G391D p.M153I ( Hcn1 G380D/+ M142I/+ mouse), severe drug-resistant neonatal- childhood-onset epilepsy, respectively. Heterozygous mice from both lines displayed spontaneous...
Abstract Hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels generate the cationic Ih current in neurons and regulate excitability of neuronal networks. The function HCN depends, part, on their subcellular localization. Of four isoforms (HCN1-4), HCN1 is strongly expressed dendrites pyramidal hippocampal area CA1 but also presynaptic terminals parvalbumin-positive interneurons (PV+ INs), which provide strong inhibitory control over activity. Yet, little known about how these...