A5019023780- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Immune responses and vaccinations
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Biochemical and Molecular Research
- Viral gastroenteritis research and epidemiology
- Toxin Mechanisms and Immunotoxins
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Virus-based gene therapy research
Jiangsu Agri-animal Husbandry Vocational College
2024-2025
Yangzhou University
2017-2023
Ministry of Agriculture and Rural Affairs
2019
Through long-term coevolution with its host, Mycobacterium tuberculosis ( M. ) uses multiple strategies to escape host defenses. The Rv0927c protein is predicted be a short-chain dehydrogenase/reductase related bacterial metabolism. However, the role of during infection remains unclear. Here, we observed that inhibited expression IL-6, TNF-α, and IL-1β, an effect dependent on NF-κB p38 pathways. Western blot analysis macrophages infected recombinant smegmatis strains showed attenuated...
Bovine tuberculosis (bTB) caused by Mycobacterium bovis is an important zoonotic disease. This infection difficult to control because of the limited ability tuberculin skin test (TST) and ancillary IFN-γ release assay detect all infected animals. In this study, we aimed develop efficient based on enzyme-linked immunospot (ELISpot) technique for diagnosis bTB, with monoclonal antibodies 3E9 Bio-labeled 6F8 used as capture detection antibodies, respectively. As expected, there were...
The Ag85 complex functions as the main secretory protein of Mycobacterium tuberculosis (M. tuberculosis) and BCG. This is comprised proteins, Ag85A, Ag85B Ag85C, with Ag85A thought to play largest role within complex. However, lack commercially available monoclonal antibodies (mAb) against still hinders biological applicative research on this protein. In study, we developed identified anti-Ag85A antibodies, five hybridoma cells were established. Using indirect immunofluorescence test, found...
Control of Mycobacterium tuberculosis (Mtb) infection requires CD4+ T-cell responses and major histocompatibility complex class II (MHC II) presentation Mtb antigens (Ags). Dendritic cells (DCs) are the most potent Ag-presenting central to initiation immune responses. Much research has indicated that DCs play an important role in anti-mycobacterial at early time points, but kinetics Ag by these during events incompletely understood. In present study, we evaluated vivo dynamics murine...
This study produced a method for the fast detection and identification of five strains infectious bronchitis virus (IBV), including currently prevalent wild-type QX G Ⅵ-1 strains, in addition to Mass, 4/91, LDT3 that are used as vaccines. Based on spike 1 (S1) glycoprotein gene sequences IBV QX, Ⅵ-1, sets customized primers, showing excellent specificity, were designed synthesized target appropriate conserved sequences. After repeated improvement, multiple PCR approach with strong...
This study aims to develop a method detect bovine multi-cytokines based on flow cytometry. Previously we have prepared and screened monoclonal antibodies against cytokines IFN-γ, IL-2, TNF-α, IP-10 MCP-1. These cytokine were fluorescently labeled, the combination of antibody cell surface molecules used for detecting multi-cytokines. Subsequently, developed was determine expression profile Mycobacterium bovis BCG infected peripheral blood mononuclear cells in vitro, evaluate level CD4+ T...