A5008778585- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- Mycobacterium research and diagnosis
- Cancer Research and Treatments
- Essential Oils and Antimicrobial Activity
- Tuberculosis Research and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Immune responses and vaccinations
- Insect Pest Control Strategies
- Hepatitis B Virus Studies
- Escherichia coli research studies
- Insect Resistance and Genetics
- Leprosy Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Cervical Cancer and HPV Research
Yangzhou University
2015-2025
Ministry of Agriculture and Rural Affairs
2019-2025
Shihezi University
2023-2024
Jiangsu Provincial Center for Disease Control and Prevention
2015-2023
Chinese Academy of Fishery Sciences
2019
Sun Yat-sen University
2019
Fifth Affiliated Hospital of Sun Yat-sen University
2019
Changchun University of Technology
2016
Affiliated Hospital of Taishan Medical University
2001
Institute of Dermatology
1999
The foodborne pathogen Listeria monocytogenes (Lm) is a highly heterogeneous species and currently comprises of 4 evolutionarily distinct lineages. Here, we characterize isolates from severe ovine listeriosis outbreaks that represent hybrid sub-lineage the major lineage II (HSL-II) serotype 4h. HSL-II are virulent exhibit higher organ colonization capacities than well-characterized hypervirulent strains Lm in an orogastric mouse infection model. harbour both Pathogenicity Island (LIPI)-1...
The adherence of Bordetella pertussis to ciliated cells and macrophages is critical colonization infection the respiratory tract. Adherence both types involves recognition eukaryotic carbohydrates by bacterial adhesin filamentous hemagglutinin (Fha). carbohydrate domain (CRD) Fha considered an important antigen for subcomponent vaccines maximize generation antiadherence antibodies capable protecting against colonization. For identification CRD Fha, a bank eight monoclonal (MAbs) that mapped...
Listeria monocytogenes (L. monocytogenes) is a ubiquitous foodborne pathogen that comprises 14 serotypes, of which serovar 4h novel serotype recently reported. Serovar L. belonging to hybrid sub-lineage II exhibit hypervirulent features. Conventional biochemical tests and widely used PCR-based serogrouping schemes could not distinguish strains. In this study, we developed new multiplex PCR assay for rapid detection monocytogenes. Three primer pairs based on the target genes, LMxysn_1095,...
The internalin family proteins, which carry the leucine repeat region structural motif, play diverse roles in Listeria monocytogenes (Lm) infection and pathogenesis. Although Internalin F, encoded by inlF , was identified more than 20 years ago, its role Lm anti-inflammatory response remains unknown. serotype 4b isolates are associated with majority of listeriosis outbreaks, but function InlF these strains is not fully understood. In this study, we aimed to elucidate modulating inflammatory...
Resistance to rice sheath blight disease that causes significant yield losses worldwide is controlled by multiple quantitative trait loci (QTL). The accurate resistance effect of qSB-9 Tq , a major QTL derived from the indica cultivar Teqing, was investigated using backcross population Teqing and Lemont (the latter as recurrent parent) set near-isogenic lines (NIL) developed in this study. A strong pathogenic Rhizoctonia solani line, RH-9, used for inoculation. Two different experimental...
Several studies have reported that intramuscular injection of DNA vaccines against infectious bronchitis virus (IBV) induces protective immune responses. In the present study, we developed oral and nasal carried S1 gene N IBV delivered by attenuated Salmonella enterica serovar Typhimurium strains SL/pV-S1 SL/pV-N, respectively. The safety stability recombinant vaccine were evaluated. Following administration to chickens, serum mucosal samples collected antibodies measured. Chickens then...
HLA-G and HLA-E are nonclassical human MHC class I molecules, which promote tolerance to NK cytotoxicity. MICA MICB known enhance the functions of T cells. However, expression these molecules has never been investigated in liver cancer. Using RT-PCR western blot, we aimed identify HLA-G, HLA-E, a panel 41 tissues dissecting from cancer patients China. mRNA was expressed 8 Human Hepatocellular Carcinoma (HCC) specimens 1 adjacent normal hepatocellular tissue. The protein found 7 mRNA-positive...
Listeria monocytogenes is a deadly foodborne pathogen, and infections can result in meningoencephalitis sepsis with mortality rates of up to 30%. In this study, we performed comparative whole-genome analysis 30 clinical isolates sequenced together 32 previously food from China. The data indicate that L. belonging the clonal complexes (CC) −1, −8, −9, −87, −121, −155 are present human cases. majority CC-87, 9, 8 overlap those CCs reported on basis multilocus sequence typing for Chinese...
ABSTRACT Listeria monocytogenes has been exploited as a vaccine carrier based upon its ability to induce strong cell-mediated immune response. At present, the safety of live, attenuated L. vaccines in patients is being studied clinical trials. also an attractive vector for use poultry; however, pathogenicity and immunogenicity this organism poultry remain be fully elucidated. In study, we investigated actA - plcB -deficient strain, yzuLM4Δ / , wild-type parent yzuLM4, avian infection model....
Tuberculosis (TB) continues to kill 1.4 million people annually, numbers of patients with drug-resistant tuberculosis increases alamingly. The vaccine is an important medical intervention in TB prevention strategies, only Bacilli Calmette-Guerin (BCG) approved worldwide for clinical use, it not ideally the protection teenagers or adults. Therefore, alternative effective and safe urgently needed. In this study, we designed a novel by using attenuated Listeria monocytogenes (LM) strain...
Listeria monocytogenes (L. monocytogenes) has been developed as a cancer vaccine vector due to its ability elicit strong innate and adaptive immune responses. For clinical application, it is necessary exploit platform strain that safe also retains immunogenicity develop candidates against cancer. In this study, highly attenuated with deletion of actA/plcB was employed deliver the human papillomavirus type 16 (HPV16) E7 antigen, which stably inserted into chromosome L. monocytogenes. The...
Cervical cancer is a leading cause of high mortality in women developing countries and has serious impact on women's health. Human papilloma virus (HPV) prophylactic vaccines have been produced may hold promise for reducing the incidence cervical cancer. However, limitations current HPV vaccine strategies make development therapeutic particularly important treatment related lesions. Our previous work demonstrated that LM4Δhly::E7 was safe effective inducing antitumor effect by...
According to the previous research of our group, we found compound ZQ-8 ((1S,2R,4S)-1,3,3-trimethylbicyclo [2.2.1]heptan-2-yl-4-(tert-butyl)benzoate). This showed a strong growth inhibitory effect on Helicoverpa armigera by inhibiting chitinase 2 and endochitinase. To further understand mechanism interfering with development H. armigera, ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) was utilized analyze metabolomics epidermis viscera after stress. The results...
The Ag85 complex functions as the main secretory protein of Mycobacterium tuberculosis (M. tuberculosis) and BCG. This is comprised proteins, Ag85A, Ag85B Ag85C, with Ag85A thought to play largest role within complex. However, lack commercially available monoclonal antibodies (mAb) against still hinders biological applicative research on this protein. In study, we developed identified anti-Ag85A antibodies, five hybridoma cells were established. Using indirect immunofluorescence test, found...
Macrophages (MΦ) and dendritic cells (DCs) are both pivotal antigen presenting capable of inducing specific cellular responses to inhaled mycobacteria, thus, they may be important in the initiation early immune mycobacterial infection. In this study, we evaluated compared roles murine splenic DCs MΦs immunity against Mycobacterium bovis Bacillus Calmette-Guérin (M.bovis BCG). The number internalized rBCG-GFP observed was obviously greater with DCs, intracellular reactive oxygen species...
Listeria monocytogenes ( Lm ) is a foodborne zoonotic pathogen that causes listeriosis with mortality rate of 20–30%. Serovar 4b and 1/2b isolates account for most outbreaks, however, no vaccine available either prophylactic or therapeutic use. Here, we developed triple-virulence-genes deletion strain, evaluated its safety, immunogenicity, cross-protective efficiency. The virulence NTSNΔ actA / plcB orfX was reduced 794-folds compared the parental strain. Additionally, it completely...
Listeria monocytogenes is a food-borne pathogen able to cause serious disease in human and animals. Listeriolysin O (LLO), major virulence factor secreted by this bacterium, vacuole-specific lysin that facilitates bacterial entrance into the host cytosol. Thus, LLO plays key role translocation intracellular spread of L. monocytogenes. To study effect on immunopotency, LLO-deficient mutant was constructed using shuttle vector followed homologous recombination. The strain had lost hemolytic...