A5101537124- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- Virus-based gene therapy research
- Plant Virus Research Studies
- Respiratory viral infections research
- Insect symbiosis and bacterial influences
- Molecular Biology Techniques and Applications
- Viral Infections and Vectors
- Transgenic Plants and Applications
- Plant and Fungal Interactions Research
- Bacteriophages and microbial interactions
- Proteins in Food Systems
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- Animal Diversity and Health Studies
- Virology and Viral Diseases
- Insect and Arachnid Ecology and Behavior
- Vector-borne infectious diseases
- Xenotransplantation and immune response
- RNA Interference and Gene Delivery
China Institute of Veterinary Drug Control
2012-2024
Xuzhou Construction Machinery Group (China)
2024
Shanxi Agricultural University
2023
Lanzhou Veterinary Research Institute
2003
Plum Island Animal Disease Center
2003
The whole-genome sequence of an African swine fever virus (ASFV) strain (HuB/HH/2019) isolated from Hubei, China, was highly similar to that the Georgia 2007/1 ASFV. After infection with strong strains, domestic pigs show typical symptoms infection, including fever, depression, reddening skin, hemorrhagic swelling various tissues, and dysfunction. earliest detoxification occurred in pharyngeal swabs at 4 days post-infection. viral load blood extremely high, ASFV detected multiple highest...
Adaptation of field isolates foot-and-mouth disease virus (FMDV) to grow in cells culture can result changes viral properties that include acquisition the ability bind cell surface heparan sulfate (HS). After 13 passages on BHK produce a vaccine, Cathay topotype isolate FMDV serotype O from China (O/CHA/90) extended its host range and bound heparin-Sepharose, although it did not require HS as receptor molecule. To understand these phenomena, we constructed chimeric viruses by using type...
During the last 12 years, a strain of foot-and-mouth disease (FMD) virus serotype O, named PanAsia, has spread from India throughout Southern Asia and Middle East. 2000, this caused outbreaks in Republic Korea, Japan, Russia (Primorsky Territory), Mongolia South Africa (KwaZulu-Natal Province), areas which experienced FMD 1934, 1908, 1964, 1974 1957, respectively. In February 2001, PanAsia to United Kingdom where, just over 7 months, it on 2030 farms. From UK, quickly Ireland, France...
Foot-and-mouth disease (FMD) is highly contagious and affects the economy of many countries worldwide. Serotype O most prevalent present in regions Asia. Lineages O/SEA/Mya-98, O/Middle East-South Asia (ME-SA)/PanAsia, O/Cathay O/ME-SA/Ind-2001 have been circulating Asian countries. Low antigenic matching between strains current vaccine makes difficult to control, therefore, analyzing molecular evolution, diversity, host tropisms FMDV may be helpful. Our results indicate that Cathay, ME-SA,...
Abstract Background Classical swine fever (CSF), caused by the virus (CSFV), is an Office International des Epizooties (OIE) notifiable disease. However, we are far from fully understand distribution, tissue tropism, pathogenesis, replication and excretion of CSFV in pigs. In this report, investigated dynamic distribution tropism internal organs experimentally infected pigs using real-time RT-PCR immunohistochemistry (IHC). Results A relative quantification PCR was established used to detect...
Abstract Background Originating in Africa, African swine fever (ASF) was introduced to China 2018. This acute and highly virulent infectious disease affects domestic pigs. The World Organization for Animal Health has listed it as a statutory reportable disease, category A disease. Methods Primers probes were designed four ASFV genes ( B646L , EP402R MGF505-3R A137R ). primers/probes conserved compared with the gene sequences of 21 strains. Results After optimization, calibration curve showed...
The attenuated C-strain vaccine against classical swine fever virus (CSFV) is one of the safest and most effective vaccines. However, little known host immune response after vaccination with vaccine. Blood samples from vaccinated pigs were collected to evaluate number cells, level specific CSFV antibody, related cytokines induced by nucleic acid was gradually removed antibody kept increasing; amount lymphocyte, Tc cell, Th cell increased; some inflammatory such as interleukin (IL)-1 tumor...
Classical swine fever (CSF) is a highly contagious fatal infectious disease caused by classical virus (CSFV). A better understanding of CSFV replication important for the study pathogenic mechanism CSF. With development novel RNA in situ Hybridization method, quantitatively localization and visualization molecular cultured cell or tissue section becomes very tool to address these pivotal questions. In this study, we established ViewRNA ISH method reveal dynamic distribution PK15 cells. We...
With the implementation of C-strain vaccine, classical swine fever (CSF) has been under control in China, which is currently a chronic atypical epidemic situation. African (ASF) emerged China 2018 and spread quickly across country. It presently occurring sporadically due to lack commercial vaccines farmers' increased awareness biosafety. Atypical porcine pestivirus (APPV) was first detected Guangdong Province, 2016, mainly harms piglets local situation southern China. These three diseases...
The G-H loop of the foot-and-mouth disease virus (FMDV) virion contains certain dominant immunogenic epitopes, as well an arginine-glycine-aspartic acid (RGD) motif that is recognized by cell surface integrin receptors. Previous experiments indicate it critical to maintain structural integrity when inserting exogenous epitope into FMDV protein. However, remains be determined how factors such different insertion positions affect interactions among virus, cells and host immune system. In this...
Abstract Single B-cell antibody generation technology is an advanced method that offers several advantages, including rapid production, high efficiency, and yield. The antibodies generated via this technique retain their natural conformation are well suited for applications in pathogen diagnosis, disease treatment, investigations of virus cross-species transmission mechanisms. Our study aimed to establish a platform generating single specifically targeting the foot-and-mouth (FMDV) 146S...
This study produced a method for the fast detection and identification of five strains infectious bronchitis virus (IBV), including currently prevalent wild-type QX G Ⅵ-1 strains, in addition to Mass, 4/91, LDT3 that are used as vaccines. Based on spike 1 (S1) glycoprotein gene sequences IBV QX, Ⅵ-1, sets customized primers, showing excellent specificity, were designed synthesized target appropriate conserved sequences. After repeated improvement, multiple PCR approach with strong...
This paper aims to detect the antigens in porcine circovirus type 2 (PCV2) vaccines by high-performance size-exclusion chromatography (HPSEC) coupled with multi-angle laser light scattering (MALLS). With purified inactivated PCV2 and virus-like particles (VLP) as references, two (a b) VLP (c d) for from four manufacturers were analyzed HPSEC-MALLS after demulsification. The antigen peaks identified test strips, Western blotting transmission electron microscope (TEM). repeatability linearity...
Abstract Background Classical swine fever (CSF), African (ASF), and atypical porcine pestivirus (APPV) are acute, virulent, contagious viral diseases currently hampering the pig industry in China, which result mummification or stillbirths piglets mortality pigs. Diagnostic assays for differentiation of infection vaccination CSFV, addition to detection ASFV APPV, urgently required better prevention, control, elimination these China. Methods A quadruple PCR-based gene microarray assay was...
We developed a pre-treatment method to remove interfering substances during quantification of 146S antigens in foot-and-mouth disease (FMD) vaccines by high performance size exclusion chromatography (HPSEC). Three methods, including ultracentrifugation, PEG precipitation and nuclease digestion, were optimized compared for removal efficiency the impurities FMD vaccines. Under conditions, contents two batches determined be 7.1 7.6 μg/mL 9.7 10.4 precipitation, 10.5 digestion. The optimal...