A5091143478- Endodontics and Root Canal Treatments
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Dental Radiography and Imaging
- Oral microbiology and periodontitis research
- Osteoarthritis Treatment and Mechanisms
- Dental Implant Techniques and Outcomes
- Tissue Engineering and Regenerative Medicine
- Dental Health and Care Utilization
- Dental Trauma and Treatments
- Bone and Dental Protein Studies
- Bone Metabolism and Diseases
- Proteoglycans and glycosaminoglycans research
- Dental Erosion and Treatment
- Peroxisome Proliferator-Activated Receptors
- Dental materials and restorations
- dental development and anomalies
- Ubiquitin and proteasome pathways
- Materials Engineering and Processing
- Protein Hydrolysis and Bioactive Peptides
- DNA Repair Mechanisms
- Psidium guajava Extracts and Applications
- Polymer Synthesis and Characterization
- Collagen: Extraction and Characterization
Institut Ilmu Kesehatan Bhakti Wiyata
2022-2025
Airlangga University
2011-2020
Hiroshima University
2001-2016
Abstract We isolated and expanded BMSCs from human alveolar/jaw bone at a high success rate (70%). These cells had potent osteogenic potential in vitro vivo, although their chondrogenic adipogenic was less than that of iliac cells. Introduction: Human marrow stromal (BMSCs) have osteogenic, chondrogenic, potential, but aspiration crest is an invasive procedure. Alveolar may be more useful for regenerative medicine, because the can aspirated alveolar with minimal pain. Materials Methods: In...
The mRNA level of basic helix-loop-helix transcription factor DEC1 (BHLHB2)/Stra13/Sharp2 was up-regulated during chondrocyte differentiation in cultures ATDC5 cells and growth plate chondrocytes, cartilage vivo. Forced expression induced chondrogenic differentiation, insulin increased this effect overexpression. Parathyroid hormone (PTH) PTH-related peptide (PTHrP) suppressed the cells, but overexpression antagonized inhibitory action PTH/PTHrP. Transforming factor-β or bone morphogenetic...
Traumatic ulcers are one of the most common mucosal diseases. Ulcerated surfaces commonly formed as a result injuries involving soft tissues oral cavity. Injuries can occur events such accidentally biting oneself while speaking or sleeping. The goal this study was to see if there were any variations in IL-6 expression after Wistar rats treated topically with probiotic Lactobacillus casei Shirota treat traumatic ulcers. Research methods24 healthy male weighing 175-250 grams and aged 2-3...
Dec2, a member of the basic helix-loop-helix (bHLH) superfamily, has been shown to function as transcriptional repressor and is implicated in cell proliferation differentiation. In addition, Dec2 transcripts exhibit striking circadian oscillation suprachiasmatic nucleus. To identify molecular mechanisms by which regulates gene expression, we carried out structure-function analyses. Gel retardation luciferase assays showed that well its related protein Dec1, preferentially binds class B E-box...
Post-tooth extraction socket preservation is necessary due to alveolar bone resorptive patterns through regenerative dentistry approaches that involve the use of stem cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth (SHED) are known potentially possess osteogenic ability. Meanwhile, carbonate apatite (CAS) can act as a biocompatible capable supporting mesenchymal (MSCs) proliferate differentiate optimally. The aim this study investigate expression...
A sample of 308 extracted human permanent maxillary first molars from an Indonesian population was randomly selected. rhomboid access cavity made in all teeth anticipation identifying a second mesiobuccal canal (MB2). Ultrasonic tips were used to open the subpulpal groove locate root. Dentin carefully removed trifurcation area at expense mesial wall. If located, 0.8 C+ file inserted into it until reached apex. The prevalence root molar 68.5% (95% CI: 63.1%-73.4%) this population. Sections...
Tissue engineering using Stem cell from Human Exfoliated Deciduous Teeth (SHED) and a natural biomaterials biomaterial scaffold has become promising therapy for the alveolar bone defect. The aim of this study was to analyze Osteoprotegerin (OPG) Receptor Activator NF-Κb ligand (RANKL) expression after application Hydroxyapatite SHED.A laboratory experimental research with post test-only control group. 14 male Wistar rats weighing 260 280 g were used as animal study. animals randomly assigned...
Background:Enterococcus faecalis (E. faecalis) is a microorganism that commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity living environments with high salt levels, temperature, and pH broad spectrum. Bacteria biofilms form one adaptive process allows bacteria survive an environment low nutrients root canals. have different from planktonic form, resistance phagocytic cells drugs, can effect persistent infection....
Background: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite scaffold (CAS) may have multiple functions that could be used to regenerate the alveolar bone defects. The purpose of this study is examine ability SHED and CAS defects using an immunohistochemical analysis. Methods: ten three-month-old healthy male Wistar rats (R. novergicus) weighed between 150-250 grams (g) were as animal models. A simple blind random sampling method was select sample assigned...
Sodium hypochlorite (NaOCl) irrigation is critical for the success of endodontic treatment and several agitation techniques have been developed to improve efficacy this irrigation. Using a combination contrast medium radiographic examination, study evaluated NaOCl extrusion during irrigant. Development pressure, which may result in apical irrigant, has described laser-activated (LAI) passive ultrasonic (PUI).We examined 40 single root canals categorized as having open apices with lesions...
Bone defect is still a major problem in dentistry. Solving this problem, tissue engineering becomes one of the promising methods for bone regeneration. One main component scaffolds. Hydroxyapatite (HA) and natural polymer are widely used scaffolds due to similarity bone. In study, author prepared from bovine hydroxyapatite (BHA), gelatin (GEL) chitosan (K). The were analyzed by using FTIR SEM-EDX. addition, its swelling degradation rate also included analysis. results (1) reveals...
Dental pulp cells (DPCs) are a promising source of transplantable in regenerative medicine. However, DPCs have not been fully characterized at the molecular level. The aim present study was to distinguish from various source-derived mesenchymal stem (MSCs), fibroblasts (FBs) and other by expression several DPC-characteristic genes. were isolated human tissues explant method or enzyme digestion method, maintained with media containing 10% serum 7.5% platelet-rich plasma. RNA dental tissue...
<ns4:p><ns4:bold>Background</ns4:bold>: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite scaffold (CAS) may have multiple functions that could be used to regenerate the alveolar bone defects. The purpose of this study is examine ability SHED and CAS defects using an immunohistochemical analysis.</ns4:p><ns4:p> <ns4:bold>Methods</ns4:bold>: ten three-month-old healthy male Wistar rats <ns4:italic>(R. novergicus</ns4:italic>) weighed between 150–250 grams (g)...
Differentiated embryo chondrocyte 2 (DEC2) is a basic helix-loop-helix-Orange transcription factor that regulates cell differentiation in various mammalian tissues. DEC2 has been shown to suppress the of mesenchymal stem cells (MSCs) into myocytes and adipocytes. In present study, we examined role chondrogenic human MSCs. The overexpression exerted minimal effects on proliferation MSCs monolayer cultures with growth medium under undifferentiating conditions, whereas it suppressed increases...
Membrane-bound transferrin-like protein (MTf), a glycosylphosphatidylinositol-anchored protein, is expressed at high levels in many tumors and several fetal adult tissues including cartilage the intestine, as well amyloid plaques of Alzheimer's disease, although its role remains unknown. MTf one major concanavalin A-binding proteins cell surface. In this study, we examined effects anti-MTf antibodies A on shape gene expression, using cultures chondrocytes MTf-overexpressing ATDC5 C3H10T1/2...
Membrane-bound transferrin-like protein (MTf) is expressed in parallel with the expression of cartilage-characteristic genes during differentiation chondrocytes, and MTf level much higher cartilage than other tissues. To investigate role cartilage, we examined effects growth factors on mouse prechondrogenic ATDC5 cells effect overexpression pluripotent mesenchymal C3H10T1/2 cells. In cultures, bone morphogenetic protein-2 transforming factor-beta as well insulin induced mRNA when these...
Dental pulp stem cells (DPSCs) can be used as a component in the formation of regenerative dentine during direct capping therapy. Concanavalin A (ConA) is type lectin with molecular weight 26 kDa derived from Canavalia ensiformis plant. Lectins possess strong proliferation and differentiation abilities various animal including lymphocytes, osteoblasts, chondrocytes. The aim study was to determine effect ConA on osteogenic DPSCs vitro. In this vitro study, were isolated third molars before...
Background: Propolis is known to have beneficial effects, namely anti- bacterial, anti-viral, anti-inflammatory, antioxidant, and immunomodulatory. extracts with anti-inflammatory properties are expected be useful in treating inflamed pulp tissue a diagnosis of reversible pulpitis. The inflammation caused by bacteria, Lactobacillus acidophilus. This research used odontoblast like cells derived from human third molars. Odontoblast exposed achidophilus were as model proinflammatory cytokine...
Induction of the proliferation and differentiation stem cells could represent a viable alternative therapeutic method for treating bone diseases. Stem are essential to tissue regeneration; although, their availability is limited. One possible increasing number promote osteogenic application red flesh dragon fruit extract supplement. The present study was performed identify analyze marrow-derived mesenchymal (BMMSCs) after exposure extract.This in vitro posttest only control group design. Red...