A5062896431- T-cell and B-cell Immunology
- Advanced Radiotherapy Techniques
- Immune Cell Function and Interaction
- Systemic Lupus Erythematosus Research
- T-cell and Retrovirus Studies
- Genetic Associations and Epidemiology
- Vector-Borne Animal Diseases
- Radiation Therapy and Dosimetry
- Immunodeficiency and Autoimmune Disorders
- Atherosclerosis and Cardiovascular Diseases
- Animal Disease Management and Epidemiology
- Analytical Chemistry and Chromatography
- Rheumatoid Arthritis Research and Therapies
- Mast cells and histamine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cytokine Signaling Pathways and Interactions
- Mass Spectrometry Techniques and Applications
- Prostate Cancer Treatment and Research
- Mesenchymal stem cell research
- Vasculitis and related conditions
- Peptidase Inhibition and Analysis
- Prostate Cancer Diagnosis and Treatment
- Pulmonary Hypertension Research and Treatments
- Cancer, Hypoxia, and Metabolism
- vaccines and immunoinformatics approaches
Kaiser Permanente
2025
CTI Engineering (Japan)
2024
Kyoto University
2011-2023
Hyogo Ion Beam Medical Center
2004-2016
Center for Genomic Science
2014
Hiroshima University
1999-2009
Shimizu (Japan)
2009
Hiroshima Institute of Technology
2005
Japanese Red Cross Society, Japan
2001
Whole-genome and -exome resequencing using next-generation sequencers is a powerful approach for identifying genomic variations that are associated with diseases. However, systematic strategies prioritizing causative variants from many candidates to explain the disease phenotype still far being established, because population-specific frequency spectrum of genetic variation has not been characterized. Here, we have collected exomic 1208 Japanese individuals through collaborative effort,...
The accurate typing of human leukocyte antigen (HLA) alleles is critical for a variety medical applications, such as genomic studies multifactorial diseases, including immune system and inflammation-related disorders, donor selection in organ transplantation regenerative medicine. Here, we developed new algorithm determining HLA using next-generation sequencing (NGS) results. method consists constructing an extensive dictionary alleles, precise mapping the NGS reads, calculating score based...
Abstract We isolated and expanded BMSCs from human alveolar/jaw bone at a high success rate (70%). These cells had potent osteogenic potential in vitro vivo, although their chondrogenic adipogenic was less than that of iliac cells. Introduction: Human marrow stromal (BMSCs) have osteogenic, chondrogenic, potential, but aspiration crest is an invasive procedure. Alveolar may be more useful for regenerative medicine, because the can aspirated alveolar with minimal pain. Materials Methods: In...
Abstract Objective Although interleukin‐17 (IL‐17)–producing γ/δ T cells were reported to play pathogenic roles in collagen‐induced arthritis (CIA), their characteristics remain unknown. The aim of this study was clarify whether or CD4+ are the predominant IL‐17–producing cells, and determine what stimulates secret IL‐17 mice with CIA. involvement SKG autoimmune patients rheumatoid (RA) also investigated. Methods affected joints CIA counted by intracellular cytokine staining during 6...
Although ex vivo expanded mesenchymal stem cells (MSC) have been used in numerous studies, the molecular signature and distribution status of MSC remain unknown. To address this matter, we identified human MSC‐characteristic genes—including nine transcription factor genes —using DNA microarray real‐time RT‐PCR analyses: Most were down‐regulated 24 h after incubation with osteogenesis‐, chondrogenesis‐ or adipogenesis‐induction medium, 48–72 knockdown factors. Furthermore, knockdowns ETV1,...
Human T-cell leukemia virus type 1 (HTLV-1) infects mainly CD4+CCR4+ effector/memory T cells in vivo. However, it remains unknown whether HTLV-1 preferentially these or this converts infected precursor to specialized cells. Expression of viral genes vivo is critical study replication and proliferation Therefore, we first analyzed gene expression non-human primates naturally with simian (STLV-1), whose virological attributes closely resemble those HTLV-1. Although the tax transcript was...
Takayasu arteritis (TAK) is a systemic vasculitis with severe complications that affects the aorta and its large branches. HLA-B*52 an established susceptibility locus to TAK. To date, there are still only limited number of reports concerning non-HLA loci We conducted genome-wide association study (GWAS) follow-up in total 633 TAK cases 5,928 controls. A 510,879 SNPs were genotyped, 5,875,450 imputed together HLA-B*52. Functional annotation significant loci, enhancer enrichment, pathway...
FRP physically interacts with CD14 by fluorescence‐activated cell sorting (View interaction)
Interstitial lung disease (ILD) is a common complication and sometimes prognostic factor of connective tissue diseases (CTDs) in humans. However, suitable animal model severe CTD-associated ILD (CTD-ILD) has been limited. In this study, we showed that zymosan-treated SKG mice developed not only arthritis but also chronic-progressive with high mortality over several months. The pathological clinical features were similar to human CTD-ILD. mouse was characterized by massive infiltration Th17...
A previous study revealed the association between susceptibility to Takayasu arteritis (TAK) and a single nucleotide polymorphism (SNP) rs6871626 located in IL12B, which encodes interleukin (IL)-12p40, common component of IL-12p70 IL-23. We investigated expression these cytokines patients with TAK, stratifying them into those or without risk allele at SNP.Plasma levels IL-12p40, IL-12p70, IL-23 were quantified 44 TAK 19 healthy controls (HCs) by enzyme-linked immunosorbent assays. Monocytes...
Rheumatoid arthritis (RA) is a major cause of adult chronic inflammatory and typical complex trait. Although several genetic determinants have been identified, they account for only part the susceptibility. We conducted genome-wide association study RA in Japanese using 225,079 SNPs genotyped 990 cases 1,236 controls from two independent collections (658 934 collection1; 332 302 collection2), followed by replication studies additional (874 855 collection3; 1,264 948 collection4). showing...
Pulmonary arterial hypertension (PAH) is a severe lung disease with only few effective treatments available. Familial cases of PAH are usually recognized as an autosomal dominant disease, but incomplete penetrance the makes it difficult to identify pathogenic variants in accordance Mendelian pattern inheritance.To elucidate complex genetic basis PAH, we obtained whole exome- or genome-sequencing data 17 subjects from 9 families heritable and applied gene-based association analysis index...
HTLV-1-associated myelopathy (HAM/TSP) is a chronic and progressive inflammatory disease of the central nervous system. The aim our study was to identify genetic determinants related onset HAM/TSP in Japanese population. We conducted genome-wide association comprising 753 patients 899 asymptomatic HTLV-1 carriers. also performed comprehensive genotyping HLA-A, -B, -C, -DPB1, -DQB1, -DRB1 genes using next-generation sequencing technology for 651 804 A strong observed HLA class I (P = 1.54 ×...
Objective While antinuclear antibodies (ANAs) are observed in healthy populations as well patients with autoimmune diseases such systemic lupus erythematosus (SLE), the detailed genetic background of ANAs has remained unclear. We undertook this study to identify determinants general population order elucidate underlying mechanisms ANA production and distinguish disease susceptibility genes from genes. Methods A total 9,575 Japanese volunteers were registered, their levels quantified using...
Calpain, a calcium-dependent cysteine protease, is reportedly involved in the pathophysiology of autoimmune diseases such as rheumatoid arthritis (RA). In addition, autoantibodies against calpastatin, natural and specific inhibitor calpain, are widely observed RA. We previously reported that E-64-d, membrane-permeable protease inhibitor, effective treating experimental arthritis. However, exact role calpastatin-calpain balance primary inflammatory cells remains unclear. Here we investigated...
<h3>Background</h3> Prostate-specific antigen (PSA) is a useful marker for prostate cancer (PCa) and widely used screening of PCa. Previous studies have shown that genetic components influence the levels PSA, some these would lead to better diagnostic sensitivity specificity However, PSA from Asian countries are limited. Our aim was identify influencing in Japanese population using genome-wide association study (GWAS) analyse whether abilities <h3>Methods</h3> We performed GWAS comprising...
Abstract A nano‐electrospray ionization (nanoESI) emitter for analysis of a biological solution was developed by packing nanoESI needle with two types resins desalting and preconcentration target molecules. Determination secreted histamine serotonin molecules in cell culture buffers demonstrated using 5‐methyltryptamine as internal standard. The results showed good linearity signals the concentration range from 0.25 to 50.0 ng/mL or serotonin. These were monitored be A23187 (calcium...
NOD/LtSzscid/IL‐2Rγ −/− (NSG) mice have advantages in establishing humanized mouse models. However, transferring human PBMCs into these often causes lethal GVH disease. In this study, we discovered an improved method for the engraftment of normal or pathological NSG and examined subsequent induction specific immune responses. We sequentially transferred CD4 + memory T (Tm) B cells obtained from healthy adults patients with autoimmune diseases mice. Removing naïve allowed successful without...