A5037164361- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Adenosine and Purinergic Signaling
- Cancer therapeutics and mechanisms
- RNA modifications and cancer
- DNA Repair Mechanisms
- Nanoparticle-Based Drug Delivery
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Consumer Perception and Purchasing Behavior
- SARS-CoV-2 and COVID-19 Research
- Cannabis and Cannabinoid Research
- Angiogenesis and VEGF in Cancer
- Ginseng Biological Effects and Applications
- Customer Service Quality and Loyalty
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Consumer Behavior in Brand Consumption and Identification
- Autophagy in Disease and Therapy
- Respiratory viral infections research
University of Massachusetts Chan Medical School
2021-2024
Sookmyung Women's University
2023-2024
Seoul National University
2022
The continuous evolution of SARS-CoV-2 variants complicates efforts to combat the ongoing pandemic, underscoring need for a dynamic platform rapid development pan-viral variant therapeutics. Oligonucleotide therapeutics are enhancing treatment numerous diseases with unprecedented potency, duration effect, and safety. Through systematic screening hundreds oligonucleotide sequences, we identified fully chemically stabilized siRNAs ASOs that target regions genome conserved in all concern,...
Abstract Ninjurin1 (Ninj1), an adhesion molecule, regulates macrophage function in hyaloid regression, multiple sclerosis, and atherosclerosis. However, its biological relevance the mechanism underlying vascular network integrity have not been studied. In this study, we investigated role of Ninj1 physiological (postnatal vessel formation) pathological (endotoxin-mediated inflammation diabetes) conditions developed a strategy to regulate using specific micro (mi)RNAs under conditions....
The lung is a complex organ with various cell types having distinct roles. Antisense oligonucleotides (ASOs) have been studied in the lung, but it has challenging to determine their effectiveness each type due lack of appropriate analytical methods. We employed three approaches study silencing efficacy within different types. First, we used lineage markers identify flow cytometry, and simultaneously measured ASO-induced cell-surface proteins CD47 or CD98. Second, applied single-cell RNA...
Reliable detection and quantification of antisense oligonucleotides (ASOs) in experimental clinical specimens are essential to understand the biological function novel oligonucleotide-based therapeutics. In this study, we describe a method detect quantify ASOs samples, whereby ASO acts as splint direct ligation complementary probes quantitative real-time PCR was used monitor products. Low levels 2'-O-methoxyethyl (2'-O-MOE) gapmer serum, liver, kidney, lung, heart, muscle, brain tissues can...
Small interfering RNAs (siRNAs) are revolutionizing the treatment of liver-associated indications. Yet, robust delivery to extrahepatic tissues remains a challenge. Conjugating lipids (e.g., docosanoic acid [DCA]) siRNA supports delivery, but tissue accumulation lower than that achieved in liver by approved therapeutics. Early evidence suggests functionalizing DCA with head group phosphatidylcholine [PC]) may enhance certain tissues. Here, we report first systematic evaluation effect PC...
While an increasing number of siRNA therapies are reaching the market, challenge efficient extra-hepatic delivery continues to limit their full therapeutic potential. Drug vehicles and hydrophobic conjugates being employed overcome bottleneck. Previously, we reported a novel dendritic conjugate that can be appended efficiently oligonucleotides, allowing them bind albumin with nanomolar affinity. Here, explore ability this albumin-binding improve siRNAs in vivo. We demonstrate binds...
CRISPR-based genome-editing technologies, including nuclease editing, base and prime have recently revolutionized the development of therapeutics targeting disease-causing mutations. To advance assessment genome editing tools, a robust mouse model is valuable, particularly for evaluating
Abstract Expansions of a G4C2 repeat in the C9ORF72 gene are most common genetic cause amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), two devastating adult-onset neurodegenerative disorders. Proposed disease mechanisms include gain toxic functions repeats, implying that selective reduction levels repeat-containing transcripts would represent treatment strategy for this disorder. In present study, using C9-ALS/FTD patient derived cells BAC transgenic mice, we have...
This study is to examine the factors affecting success of business withhigh accomplishments in customer recognition and financial aspect fashion industry. In addition, through survey on consumers interviews industry officials who concerned brand, perceived were compared between consumer groups. selected a total 20 brands, which 5 brands per 4 categories(e.g., women, men, sports casual wear), with high satisfaction loyalty consumer's perspective revenues, revenue growth, profit rate 2004...
A well-controlled inflammatory response is crucial for the recovery from injury and maintenance of tissue homeostasis. The anti-inflammatory 2-methoxycinnamaldehyde (2-MCA), a natural compound derived cinnamon, has been studied; however, underlying mechanism on macrophage not fully elucidated. In this study, LPS-stimulated production TNF-α NO was reduced by 2-MCA in macrophages. significantly activated NRF2 pathway, expression levels autophagy-associated proteins macrophages, including LC3...
Abstract Reliable detection and quantification of antisense oligonucleotides (ASOs) in experimental clinical specimens is essential to understand the biological function novel oligonucleotide-based therapeutics. In this study, we describe a method detect quantify ASOs samples, whereby ASO acts as splint direct ligation complementary probes quantitative real-time PCR was used monitor products. Low levels 2′- O -MOE gapmer serum, liver, kidney, lung, heart, muscle, brain tissues can be...