A5018929645- S100 Proteins and Annexins
- Traumatic Brain Injury and Neurovascular Disturbances
- Alzheimer's disease research and treatments
- Acute Ischemic Stroke Management
- Parkinson's Disease Mechanisms and Treatments
- Amyotrophic Lateral Sclerosis Research
- Advanced MEMS and NEMS Technologies
- Multiple Sclerosis Research Studies
- Skin and Cellular Biology Research
- Dementia and Cognitive Impairment Research
- HIV Research and Treatment
- Traumatic Brain Injury Research
- Cerebrovascular and Carotid Artery Diseases
- Prion Diseases and Protein Misfolding
- Cardiac Arrest and Resuscitation
- Neuroinflammation and Neurodegeneration Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- Neurological disorders and treatments
- Electrochemical sensors and biosensors
- Cell Adhesion Molecules Research
- Advanced Neuroimaging Techniques and Applications
- Silicon Nanostructures and Photoluminescence
- Neurological diseases and metabolism
- HIV/AIDS Research and Interventions
- Cerebrovascular and genetic disorders
Sahlgrenska University Hospital
2010-2024
Karolinska Institutet
1996-2022
Karolinska University Hospital
2007-2022
Bristol-Myers Squibb (Sweden)
2022
University of Gothenburg
2010-2021
Lund University
1986-2015
In-Q-Tel
2015
Uppsala University
1993-2014
University College London
2014
San Francisco VA Medical Center
2014
Abstract: In the present study we describe an ELISA to quantify light subunit of neurofilament triplet protein (NFL) in CSF. The method was validated by measuring CSF NFL concentrations healthy individuals and two well‐characterized groups patients with amyotrophic lateral sclerosis (ALS) Alzheimer's disease (AD). levels were increased ALS (1,743 ± 1,661 ng/L; mean SD) AD (346 176 ng/L) compared controls (138 31 p < 0.0001 for both). Within group, lower motor neuron signs only had (360...
Abstract Background: To simultaneously study several biomarkers for Alzheimer disease (AD), we used the xMAP™ technology to develop and evaluate a multiparametric bead-based assay quantification of β-amyloid(1–42) [Aβ(1–42)], total tau (T-TAU), hyperphosphorylated [P-TAU(181P)] in cerebrospinal fluid (CSF). Methods: We compared new multianalyte format with established ELISA techniques same proteins. then performed clinical using CSF samples from patients AD or mild cognitive impairment...
We have previously demonstrated that stroke influences systemic immune responses. The aim of the present study was to investigate patterns local inflammatory response as a consequence acute stroke.Thirty patients were studied prospectively on days 0 3, 7 9, 21 26, and after day 90 with clinical evaluations, radiological assessments, analysis serum cerebrospinal fluid cytokine levels.Significantly increased levels interleukin-6 (IL-6) in (P < .001) observed virtually all compared healthy...
Retrospective studies have found that patients receiving general anesthesia for endovascular treatment in acute ischemic stroke worse neurological outcome compared with conscious sedation. In this prospective randomized single-center study, we investigated the impact of technique on patients.Ninety 2013 to 2016 were included and or Difference at 3 months, measured as modified Rankin Scale score, was analyzed (primary outcome) early improvement National Institutes Health Stroke cerebral...
Little solid information is available on the possible risks for neuronal injury in amateur boxing.To determine whether boxing and severity of hits are associated with elevated levels biochemical markers cerebrospinal fluid.Longitudinal study.Referral center specializing evaluation neurodegenerative disorders.Fourteen boxers (11 men 3 women) 10 healthy male nonathletic control subjects.The underwent lumbar puncture 7 to days months after a bout. The subjects LP once.Neurofilament light...
Abstract Objective: The impact of present disease‐modifying treatments (DMTs) in multiple sclerosis (MS) on nerve injury and reactive astrogliosis is still unclear. Therefore, we studied the effect natalizumab treatment release 2 brain‐specific tissue damage markers into cerebrospinal fluid (CSF) MS patients. Methods: CSF samples from 92 patients with relapsing forms were collected a prospective manner prior to after 6 or 12 months. In 86 cases, was used as second‐line DMT due breakthrough...
Background. @nbsp; Cerebrovascular complications (CVCs) have remained a major therapeutic and prognostic challenge associated with infective endocarditis, definite risk factors not been fully elucidated. This prospective study was designed to the evaluate total incidence of CVC endocarditis factors. Methods. During 2 periods, from June 1998 through April 2001 September 2002 January 2005, patients were prospectively enrolled in regardless neurological symptoms. Study underwent examinations...
We hypothesized that CSF neurofilament light (NFL) levels would be elevated in dementias with subcortical involvement, including vascular dementia (VaD), but less primarily affecting gray matter structures, such as Alzheimer disease (AD), and NFL correlate severity shorter survival time irrespective of clinical diagnosis.We included 3,356 individuals who had analyzed our laboratory between 2005 2012. Clinical diagnoses Mini-Mental State Examination (MMSE) scores were obtained from the...
<b><i>Objective:</i></b> To determine if CNS-derived proteins present in the CSF of multiple sclerosis (MS) patients reflect different pathologic processes MS and these could be useful as biologic markers disease activity. <b><i>Methods:</i></b> Concentrations neurofilament light protein (NFL), glial fibrillary acidic (GFAP), S100B, neuron-specific enolase (NSE) were determined 66 50 healthy control subjects with immunoassays. <b><i>Results:</i></b> The mean levels NFL increased during all...
The neurofilament protein is a major structural of neurons and marker for axonal damage. concentrations the light subunit triplet (NFL) in CSF were significantly increased patients with relapsing-remitting multiple sclerosis compared healthy controls (p<0.001). Seventy eight per cent showed NFL concentrations. Significant correlations between concentration clinical indices discerned disability, exacerbation rate, time from start previous to lumbar puncture. results suggest that damage...
<b>Objective: </b> We investigated if tau, microtubular binding protein, in serum and ventricular CSF (vCSF) patients with severe traumatic brain injury (TBI) during the initial posttraumatic days correlated to 1-year outcome. <b>Methods: Patients TBI (n = 39, Glasgow Coma Scale score ≤8) were included. measured vCSF total tau on 0 14, using ELISA. Extended Outcome (GOSE), NIH Stroke (NIHSS) neurologic status, Bartel Daily Living Index. 20) normal pressure hydrocephalus (NPH) served as...
SUMMARY A growing body of evidence points out the potential role inflammatory mechanisms in pathophysiology ischaemic brain damage. We have recently demonstrated that stroke patients display an intrathecal production proinflammatory cytokines, such as IL-1β and IL-6 already within first 24 h after beginning symptoms (Tarkowski et al., 1995). The aim present study was to investigate patterns local responses a consequence acute stroke. Thirty were studied prospectively on days 0–3, 7–9, 21–26...
<b><i>Objective:</i></b> To evaluate levels of neurofilament light (NFL) and glial fibrillary acidic protein (GFAP) in CSF from patients with multiple sclerosis (MS) relation to clinical progress the disease. <b><i>Methods:</i></b> NFL GFAP were determined by sensitive ELISAs 99 different subtypes MS, classified terms “ongoing relapse” or “clinically stable disease,” 25 control subjects. Levels compared paraclinical data such as immunoglobulin G index inflammatory cell count CSF, related...
Background and Purpose Patients resuscitated from cardiac arrest have a high early mortality rate. Prognostic evaluation based on clinical observations is uncertain would benefit the use of biochemical markers hypoxic brain damage. The astroglial protein S-100 an established marker central nervous system injury. purpose present study was to validate serum determinations with regard outcome after arrest. Methods Levels were measured radioimmunoassay in 41 patients first 3 days out-of-hospital...
<b>Background:</b> Low levels of cerebrospinal fluid (CSF) β-amyloid 1–42 (Aβ42) and high total tau (T-tau) are diagnostic for manifest Alzheimer's disease. It is not known, however, whether these biomarkers may be risk indicators cognitive decline in otherwise healthy older people. <b>Methods:</b> The longitudinal relationship between CSF markers, Aβ42 T-tau, measured 1992, change Mini-Mental State Examination (ΔMMSE) score 1992 2002 were investigated 55 women (aged 70–84 years, mean (SD)...
We initiated the present study to evaluate clinical value of consecutive concentration determinations S-100 and glial fibrillary acidic proteins in cerebrospinal fluid from patients with brain infarction. took sequential samples 28 within 48 hours, at 7 days, 18-21 days after ictus. measured astroglial protein concentrations using an enzyme-linked immunosorbent assay also determined size infarction (computed tomography), state patient (simplified activities daily living test), blood-brain...
Abstract The objective of this study was to compare cerebrospinal fluid (CSF) levels the light subtype neurofilament proteins (NFL), tau, and β‐amyloid42 (Aβ42) in individuals with moderate or severe white matter changes (WMC) those mild no WMC. Twenty‐two patients Alzheimer's disease (AD), nine subcortical vascular dementia (SVD), 20 normal controls were included study. occurrence WMC evaluated by a neuroradiologist using Blennow‐Wallin scale. Thirty‐seven subjects had only punctate WMC; 14...
Prevalence of neurocognitive impairment in HIV-1 infected patients is reported to be high. Whether this a result active HIV-related neurodegeneration unclear. We examined axonal injury by measuring the light subunit neurofilament protein (NFL) CSF with novel, sensitive method.With cross-sectional design, concentrations (marker neuronal injury), neopterin (intrathecal immunoactivation) and CSF/Plasma albumin ratio (blood-brain barrier integrity) were analyzed on from 252 HIV-infected...