A5030132309- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- Cellular transport and secretion
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- RNA Interference and Gene Delivery
- Fatty Acid Research and Health
- Fungal and yeast genetics research
- Pesticide and Herbicide Environmental Studies
- Enzyme Catalysis and Immobilization
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Nanopore and Nanochannel Transport Studies
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Bacterial Genetics and Biotechnology
Saarland University
2023-2025
Utrecht University
2015-2024
University of Oxford
2019-2022
Radboud University Nijmegen
2015
Radboud University Medical Center
2015
Abstract Lipid droplets (LDs) are universal lipid storage organelles with a core of neutral lipids, such as triacylglycerols, surrounded by phospholipid monolayer. This unique architecture is generated during LD biogenesis at endoplasmic reticulum (ER) sites marked Seipin, conserved membrane protein mutated in lipodystrophy. Here structural, biochemical and molecular dynamics simulation approaches reveal the mechanism formation yeast Seipin Sei1 its partner Ldb16. We show that luminal domain...
Lipid droplets (LDs) are essential for cellular lipid homeostasis by storing diverse neutral lipids (NLs), such as triacylglycerol (TAG), steryl esters (SE), and retinyl (RE). A proper assembly of TAG-containing LDs at the ER requires Seipin, a conserved protein often mutated in lipodystrophies. Here, we show that yeast Seipin Sei1 its partner Ldb16 also promote storage other NL LDs. Importantly, this role Sei1/Ldb16 is evolutionarily expression human-Seipin restored normal SE-containing...
The impact of the genetic background on lipidome yeast strains remains underexplored. This study systematically compares lipidomes five commonly used laboratory strains: BY4741, W303, D273-10B, RM11-1a, and CEN.PK2-1c. Shotgun lipidomics reveals significant variations in lipid class acyl chain composition down to level molecular species. Notably, most abundant differed between phosphatidylinositol (PI) lipids are predominant while phosphatidylethanolamine (PE) D273. Ergosterol esters, which...
Mitochondria depend on the import of phospholipid precursors for biosynthesis phosphatidylethanolamine (PE) and cardiolipin, yet mechanism their transport remains elusive. A dynamic lipidomics approach revealed that mitochondria preferentially di-unsaturated phosphatidylserine (PS) subsequent conversion to PE by mitochondrial PS decarboxylase Psd1p. Several protein complexes tethering endomembrane system have been implicated in lipid yeast, including endoplasmic reticulum (ER)-mitochondrial...
Styrene-maleic acid copolymers (SMA) have been gaining interest in the field of membrane research due to their ability solubilize membranes into nanodics. The SMA molecules act as an amphipathic belt that surrounds nanodiscs, whereby hydrophobic styrene moieties can insert between lipid acyl chains. Here we used variants with different styrene-to-maleic ratio (i.e. 2:1, 3:1 and 4:1) investigate how packing nanodiscs is affected by presence polymers it depends on polymer composition. This was...
Article14 September 2021Open Access Source DataTransparent process Shortening of membrane lipid acyl chains compensates for phosphatidylcholine deficiency in choline-auxotroph yeast Xue Bao orcid.org/0000-0002-5129-0728 Membrane Biochemistry & Biophysics, Bijvoet Center Biomolecular Research and Institute Biomembranes, Utrecht University, Utrecht, The Netherlands Search more papers by this author Martijn C Koorengevel Marian J A Groot Koerkamp orcid.org/0000-0002-0867-0821 Princess Máxima...
Abstract The budding yeast Saccharomyces cerevisiae is a poikilothermic organism and adapts its lipid composition to the environmental temperature maintain membrane physical properties. Studies addressing temperature-dependent adaptation of lipidome in have described changes phospholipid at level sum ( e.g. PC 32:1) molecular (e.g. 16:0_16:1). However, date, there no information positional isomers 16:0/16:1 versus 16:1/16:0). In this study, combined Collision- Ozone-Induced Dissociation...
Large unilamellar liposomes (LUVs) are popular membrane models for studying the impact of lipids and bilayer properties on structure function individual proteins. The functional reconstitution transmembrane in can be challenging, especially, if hydrophobic thickness protein does not match surrounding lipid bilayer. During procedure Such mismatch causes low yields aggregation, which exacerbated sterol-rich membranes featuring compressibility. Here, we explore new approaches to reversibly tune...
Yeast is a poikilothermic organism and adapts its lipid composition to the environmental temperature maintain membrane physical properties. Studies addressing temperature‐dependent adaptation of lipidome have described changes in phospholipid at level sum (e.g. PC 32:1) molecular 16:0_16:1). However, there little information positional isomers 16:0/16:1 versus 16:1/16:0). Here, we used collision‐ ozone‐induced dissociation (CID/OzID) mass spectrometry investigate homeoviscous PC, PE PS...
Phospholipid N-methyltransferases (PLMTs) synthesize phosphatidylcholine by methylating phosphatidylethanolamine using S-adenosylmethionine as a methyl donor. Eukaryotic PLMTs are integral membrane enzymes located in the endoplasmic reticulum (ER). Recently Opi3, PLMT of yeast Saccharomyces cerevisiae was proposed to perform trans catalysis, i.e. while localized ER, Opi3 would methylate lipid substrates plasma at contact sites. Here, we tested whether active site is cytosolic side ER...
Abstract Mitochondria depend on the import of phospholipid precursors for biosynthesis non-bilayer lipids phosphatidylethanolamine (PE) and cardiolipin required proper function, yet mechanism lipid remains elusive. Pulse labeling yeast with stable isotope-labeled serine followed by mass spectrometry analysis revealed that mitochondria preferentially di-unsaturated phosphatidylserine (PS) conversion to PE mitochondrial PS decarboxylase Psd1p. Several protein complexes tethering endomembrane...
ABSTRACT Phosphatidylcholine (PC) is an abundant membrane lipid component in most eukaryotes including yeast. PC has been assigned a multitude of functions addition to that building block the bilayer. Here we show evolvable essential yeast by isolating suppressor mutants devoid exhibit robust growth. The requirement for suppressed monosomy chromosome XV, or point mutation ACC1 gene encoding acetyl-CoA carboxylase. Although these two genetic adaptations rewire biosynthesis differently, both...