A5031976046- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Dermatology and Skin Diseases
- Microfluidic and Capillary Electrophoresis Applications
- Wound Healing and Treatments
- Skin Protection and Aging
- Estrogen and related hormone effects
- Genetic Neurodegenerative Diseases
- Advanced Biosensing Techniques and Applications
- Hair Growth and Disorders
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- NF-κB Signaling Pathways
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Pharmaceutical and Antibiotic Environmental Impacts
- Garlic and Onion Studies
- Monoclonal and Polyclonal Antibodies Research
- Technology in Education and Healthcare
- Smart Cities and Technologies
- Gold and Silver Nanoparticles Synthesis and Applications
- Muscle Physiology and Disorders
- SARS-CoV-2 detection and testing
- Electrowetting and Microfluidic Technologies
- Viral Infectious Diseases and Gene Expression in Insects
Institute of Advanced Chemistry of Catalonia
2018-2023
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2018-2023
Instituto de Investigación Sanitaria La Fe
2017-2021
Hospital Universitari i Politècnic La Fe
2019
Universitat Jaume I
2014
Instituto de Biomedicina de Valencia
2006-2013
Consejo Superior de Investigaciones Científicas
2010-2013
Centro de Investigacion Principe Felipe
2006-2008
Huntington disease is a neurodegenerative condition for which there no cure to date. Activation of AMP-activated protein kinase has previously been shown be beneficial in vitro and vivo models Huntington's disease. Moreover, recent cross-sectional study demonstrated that treatment with metformin, well-known activator this enzyme, associated better cognitive scores patients We performed preclinical using metformin treat phenotypes the zQ175 mouse model evaluated behavior (motor...
Health initiatives worldwide demand affordable point-of-care devices to aid in the reduction of morbidity and mortality rates high-incidence infectious noncommunicable diseases. However, production robust reliable easy-to-use diagnostic platforms showing ability quantitatively measure several biomarkers physiological fluids that could turn be decentralized reach any relevant environment remains a challenge. Here, we show particular combination paper-microfluidic technology, electrochemical...
To investigate the contribution of glucocorticoid receptor (GR) in skin development and mechanisms underlying this function, we have analyzed two mouse models which GR has been functionally inactivated: knockout GR−/− mice dimerization mutant GRdim/dim that mediates defective DNA binding-dependent transcription. Because null die perinatally, evaluated architecture late embryos by histological, immunohistochemical, electron microscopy studies. Loss function resulted incomplete epidermal...
Human dermo-epidermal skin equivalents (DE) comprising in vitro expanded autologous keratinocytes and fibroblasts are a good option for massive burn treatment. However, the lengthy expansion time required to obtain sufficient surface cover an extensive together with challenging surgical procedure limits their clinical use. The integration of DE biodegradable scaffolds has been proposed effort enhance mechanical properties. Here, it is shown that poly(hydroxybutyrate) electrospun (PHB)...
Delayed wound healing is one of the most common secondary adverse effects associated to therapeutic use glucocorticoid (GC) analogs, which act through ligand-dependent transcription factor GC-receptor (GR). GR function exerted DNA-binding-dependent and –independent mechanisms, classically referred as transactivation (TA) transrepression (TR). Currently both TA TR are thought contribute therapeutical mediated by GR; however their relative contribution unwanted side such delayed unknown. We...
We have previously shown that the glucocorticoid receptor (GR) is required for skin homeostasis and epidermal barrier competence. To understand transcriptional program by which GR regulates development, we performed a microarray analysis using of GR(-/-) GR(+/+) mice embryonic d 18.5 identified 442 differentially expressed genes. Functional clustering demonstrated overrepresentation genes involved in ectoderm/epidermis development. found strong repression encoding proteins associated with...
Glucocorticoids (GCs) play a key role in skin homeostasis and stress responses acting through the GC receptor (GR), which modulates gene expression by DNA binding-dependent (transactivation) -independent (transrepression) mechanisms. To delineate mechanisms underlie beneficial adverse effects mediated GR epidermis other epithelia, we have generated transgenic mice that express mutant (P493R, A494S), is defective for transactivation but retains transrepression activity, under control of...
In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), platinum counter electrode (CE), and reference (RE). Firstly, cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic (SA2BSA) was immobilized onto transducers surface by carbodiimide chemistry. The analyte quantified...
The glucocorticoid receptor (GR) plays a crucial role in epidermal morphogenesis during embryonic development, as demonstrated by analyzing genetically modified mouse models of GR gain- and loss-of-function. Eyelid formation constitutes useful model to study epithelial it requires coordinated regulation keratinocyte proliferation, apoptosis migration. We have analyzed this biological process GR(-/-) embryos ontogeny. Our data demonstrate that deficiency results delayed impaired eyelid...
Aggregation of mutant huntingtin, because an expanded polyglutamine track, underlies the cause neurodegeneration in Huntington disease (HD). However, it remains unclear how some alterations at cellular level lead to specific structural changes HD brains. In this context, neuroprotective effect activation AMP-activated protein kinase (AMPK) appears be a determinant factor several neurodegenerative diseases, including HD. present work, we describe series indole-derived compounds able activate...
Environmental pollutants vigilance is one of the main problems that aquaculture industry has to face with objective ensure quality their products and prevent entrance in food chain finally may arrive consumer. Contaminants such as hormones, antibiotics or biocides are especially relevant due toxicity, pharmacological effect hormonal activity can be considered harmful for final The contaminants detected environment where growing, concentration found (i.e., seawater) range µg·L−1, ng·L−1 even...
Lab-on-a-chip devices incorporating valves and pumps can perform complex assays involving multiple reagents. However, the instruments used to drive these chips are bulky. In this article, a new wax valve design that uses light from emitting diode (LED) for both opening closing is reported. The pumping chamber integrated in lab-on-a-foil be fabricated at low cost using rapid prototyping techniques. A chip implementation of enzyme-linked immunosorbent (ELISA) designed. porous nitrocellulose...
Elementary PDMS-based building blocks of fluidic, optical and optofluidic components for Lab on a chip (LOC) platforms has here been developed. All individual modules are compatible can be anchored released with the help puzzle-type connectors This approach is powerful toolbox to create modular systems (MOPS), which modified/upgraded user needs in-situ reconfigurable. In addition, PDMS locally functionalized, defining biosensor. Measurements in absorbance fluorescence have pursued as demonstrator.