A5006724579- Planarian Biology and Electrostimulation
- Cellular Mechanics and Interactions
- Marine Ecology and Invasive Species
- Marine Invertebrate Physiology and Ecology
- Wnt/β-catenin signaling in development and cancer
- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Parasites and Host Interactions
- Skin and Cellular Biology Research
- MXene and MAX Phase Materials
- Single-cell and spatial transcriptomics
- Protist diversity and phylogeny
- Interdisciplinary Research and Collaboration
- Science, Research, and Medicine
- Parasite Biology and Host Interactions
- Olfactory and Sensory Function Studies
- Extracellular vesicles in disease
- Biomedical and Engineering Education
- Helminth infection and control
Baylor College of Medicine
2022-2024
Stowers Institute for Medical Research
2019-2022
Institute for Medical Research
2022
Stanford University
2015-2016
Mechanical strain regulates the development, organization, and function of multicellular tissues, but mechanisms linking mechanical cell-cell junction proteins to cellular responses are poorly understood. Here, we showed that applied quiescent epithelial cells induced rapid cell cycle reentry, mediated by independent nuclear accumulation transcriptional activity first Yap1 then β-catenin. Inhibition Yap1- β-catenin-mediated transcription blocked reentry progression through G1 into S phase,...
A cross-sectional pilot study of hookworm infection was carried out among 292 subjects from 62 households in Kintampo North, Ghana. The overall prevalence 45%, peaking those 11–20 years old (58.5%). In children, risk factors for included coinfection with malaria and increased serum immunoglobulin G reactivity to secretory antigens. Risk adults poor nutritional status, not using a latrine, wearing shoes, occupation (farming). Although albendazole therapy associated an egg reduction rate 82%,...
Mechanical force and Wnt signaling activate β-catenin-mediated transcription to promote proliferation tissue expansion. However, it is unknown whether mechanical act independently or synergize β-catenin cell division. We show that strain induced Src-dependent phosphorylation of Y654 increased in mammalian MDCK epithelial cells. Under these conditions, cells accumulated S/G2 (independent DNA damage) but did not divide. Activating through Casein Kinase I inhibition Wnt3A addition...
ABSTRACT The field of developmental biology has declined in prominence recent decades, with off-shoots from the becoming more fashionable and highly funded. This created inequity discovery opportunity, partly due to perception that is antiquated or not cutting edge. A ‘think tank’ scientists multiple biology-related disciplines came together define specific challenges may have inhibited innovation, provide tangible solutions some issues facing biology. community suggestions include a call...
SUMMARY Stem cells enable regeneration by self-renewing and differentiating as instructed a local microenvironment called niche 1–3 . In most cases, the repair or replacement of tissues is fueled tissue-specific lineage-restricted stem that proliferate in response to injury apoptosis 4–11 However, organisms regenerate using abundant adult pluripotent cells, cell niches support tissue have not been identified characterized. Since these are often more widely distributed plentiful than other...
Abstract Regeneration requires functional coordination of stem cells, their progeny, and differentiated cells. Past studies have focused on regulation cell identity proliferation near to the wound-site, but less is known about contributions made by cells distant injury. Here, we present a comprehensive atlas whole-body regeneration over time identify rare, transient, somatic states induced injury required for regeneration. To characterize amputation-specific signaling across whole animal,...
Abstract Planarian asexual reproduction via transverse fission is a size-dependent animal behavior that offers robust context to investigate mechanisms coordinating growth, patterning, and function. During fission, planarians mechanically break off portion of posterior tissue which will go on regenerate into clonal progeny. By optimizing husbandry techniques the planarian Schmidtea mediterranea, we developed protocol robustly induces behavior. This technique permits investigation effects...
Abstract Spatial transcriptomic techniques such as Slide-seqV2 uncover novel relationships and interactions between cell types by coupling gene expression spatial data. Here we discuss two unexpected sources of error in data, one physical computational. To address this present an analysis pipeline augmentation, Syrah, which corrects for these errors show that it improves both data quantity quality over the standard alone or combination with additional sequencing.