A5066087627- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Parasitic infections in humans and animals
- Parasites and Host Interactions
- Cryospheric studies and observations
- Aeolian processes and effects
- Icing and De-icing Technologies
- Vector-Borne Animal Diseases
- Enterobacteriaceae and Cronobacter Research
- Ecology and Vegetation Dynamics Studies
- Rangeland and Wildlife Management
- Environmental Philosophy and Ethics
- Child Nutrition and Water Access
- Freshwater macroinvertebrate diversity and ecology
- Helminth infection and control
- Remote Sensing and LiDAR Applications
- Insect Resistance and Genetics
- Pneumocystis jirovecii pneumonia detection and treatment
- Soil Carbon and Nitrogen Dynamics
- Ecosystem dynamics and resilience
- Toxoplasma gondii Research Studies
- Science and Education Research
- Global Energy and Sustainability Research
- Galectins and Cancer Biology
- Forensic Entomology and Diptera Studies
Universidade Sagrado Coração
2016-2022
Universidade de Santo Amaro
2022
Universidade Estadual Paulista (Unesp)
2007-2021
Botanic Gardens and Parks Authority
2013-2016
The University of Western Australia
2016
Fundação de Apoio à Universidade Federal de São Paulo
2016
University of California, Davis
2013
Abstract Demand for restoration of resilient, self‐sustaining, and biodiverse natural ecosystems as a conservation measure is increasing globally; however, efforts frequently fail to meet standards appropriate this objective. Achieving these requires management underpinned by input from diverse scientific disciplines including ecology, biotechnology, engineering, soil science, ecophysiology, genetics. Despite research activity, gap between the immediate needs practitioners outputs science...
Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden disease is particularly high among children living developing areas the world, where transmission favored by lower hygienic standards and scarce availability safe water. However, asymptomatic infection polyparasitism are also commonly observed poor settings. Here, we investigated prevalence intestinal two small fishing villages, Porto Said (PS) Santa Maria da Serra (SM), situated along river...
The administration of viable Bifidobacterium animaliswas tested to induce resistance against Strongyloides venezuelensis infection in mice. Effects on parasite burden, worm length, egg output, and intestinal mucosal histology were evaluated. oral B. animalis, strain 04450B, starting 14 days before the inoculation nematode larvae significantly decreased burden output. In probiotic treated animals, percent reduction adult worms intestine was 33% production 21%, compared with those control...
Hookworm infection stands out for its worldwide distribution and veterinary public health relevance. Based on copromicroscopic examinations polymerase chain reaction (PCR) amplification of the ITS1-5.8S-ITS2 region, we assessed, respectively, prevalence intestinal parasites identification canine hookworm species in faeces recovered from 278 dogs living households an inland municipality São Paulo State, Brazil. Intestinal were found 67.3% was at highest rate (56.6%), followed by Toxocara...
SUMMARY Giardia duodenalis is a common intestinal parasite infecting children attending daycare centres. This study aimed to verify occurrence and the genotypes of isolates aged 0–6 years workers at centre in state São Paulo, Brazil. The families who tested positive for , were asked provide stool samples from household members their dogs. Samples (123 children, 14 employees, 44 members, 19 after treatment, 20 dogs) examined parasites using concentration methods. DNA extracted all was...
Giardia infections in captive nonhuman primates (NHP) housed at a Brazilian zoo were investigated order to address their zoonotic potential. Fresh fecal samples collected from the floors of 22 enclosures where 47 18 different species housed. The diagnosis intestinal parasites after concentration by sedimentation and flotation methods revealed following frequencies: (18%); Entamoeba spp. Endolimax nana (4.5%); Iodamoeba Oxyurid (4.5%) Strongylid (4.5%). Genomic DNA extracted all was processed...
Giardia duodenalis is one of the most important and widespread gastrointestinal parasites in world. Despite its relevance as a causative agent diarrhea, asymptomatic giardiasis occurs frequently, especially low resources settings which children are exposed to many risk factors. Based on microscopic examination polymerase chain reaction (PCR) amplification sequencing beta-giardin (bg), triose phosphate isomerase (tpi) glutamate dehydrogenase (gdh) genes, we assessed G. occurrence genetic...
In order to provide additional data on the prevalence and genetic diversity of Dientamoeba fragilis in human populations, we conducted a study children from low-income communities Sao Paulo State, Brazil. Fecal samples daycare center attendees up 6 years old (n=156) staff members (n=18) were submitted PCR sequencing D. as well microscopic examination for presence other intestinal parasites. All assessed asymptomatic 10.3% (16/156) positive fragilis. No worker was found be positive. An...
This report describes a preliminary characterization of proteolytic activity proteins isolated from lysate Giardia trophozoites an axenic Brazilian strain. Fractions obtained by high-performance liquid chromatography (FPLC) were tested in SDS-polyacrylamide gel for the protein profiles, and proteases was analyzed using gelatin impregnated SDS-PAGE. The based on inhibition assays employing synthetic inhibitors cysteine (E-64, IAA), serine (PMSF, TPCK, TLCK, elastatinal), metalo (EDTA)...
Results from our laboratory revealed propolis activity on Giardia trophozoites proliferation. Since therapeutic agents can inhibit the of proteases related to relevant biologic and physiologic processes parasites, this study was undertaken characterise proteolytic excretory/secretory products (ESP) treated with propolis. ESP obtained culture supernatants exposed 250 500 µg mL(-1) were tested in sodium dodecyl sulfate-polyacrylamide gel electrophoresis for protein profiles protease assayed...
Most alpine glaciers worldwide are receding rapidly, revealing new streams to be colonized by aquatic organisms. Several major questions whether these organisms, how fast this colonizing occurs, which organisms colonizing, and what factors constrain dispersal. We examined longitudinal patterns (over a length of 5 km) in physicochemistry, as well periphyton macroinvertebrate composition 2 glacier (Dinwoody, Gannett) the Wind River Range wilderness, home few remaining contiguous USA. found...
Abstract: The protein profiles and proteolytic activity of the excretory secretory products (E/SP) first (L1), second (L2) third (L3) larval stages Cochliomyia hominivorax were studied in laboratory. Analysis on E/SP profile was carried out using polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). each stage (L1, L2 L3) treated with protease inhibitors, 30μg, 40μg 50μg protein, applied to 10% gel. crude analyzed gels copolymerized gelatin by colorimetric assays azocasein as a...
Objective: To detect Dientamoeba (D.) fragilis and Enterocytozoon (E.) bieneusi to assess their genetic characteristics in stool samples submitted for routine examination a clinical laboratory Southeastern Brazil. Methods: In this survey, 348 from female male individuals with age ranging 0 ≥55 years were analyzed by PCR amplifying sequencing based on the small subunit ribosomal RNA (SSU rRNA) gene of D. internal transcribed spacer E. . Results: isolates observed 2.29% (8/348) 4.59% (16/348)...