A5033539059- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Synthesis and biological activity
- Ion channel regulation and function
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Multicomponent Synthesis of Heterocycles
- Nonlinear Optical Materials Research
- Microbial Metabolites in Food Biotechnology
- Ion Transport and Channel Regulation
- Glycosylation and Glycoproteins Research
- Receptor Mechanisms and Signaling
- Erythrocyte Function and Pathophysiology
- Synthesis and Biological Evaluation
- Synthesis and Characterization of Heterocyclic Compounds
- Lipid Membrane Structure and Behavior
- Enzyme Catalysis and Immobilization
- Sperm and Testicular Function
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- Reproductive Biology and Fertility
- Microbial bioremediation and biosurfactants
- Probiotics and Fermented Foods
- Synthesis of heterocyclic compounds
- Protein Structure and Dynamics
Sikkim University
2020-2025
Centre for Human Genetics
2006-2024
University of Oxford
2006-2024
Swami Keshwanand Rajasthan Agricultural University
2023
Maharishi Markandeshwar University, Mullana
2019-2023
Govind Ballabh Pant University of Agriculture and Technology
2022
Immunocore (United Kingdom)
2020-2022
Institute Of Pesticides Formulation Technology
2021
Dr. Yashwant Singh Parmar University of Horticulture and Forestry
2019
University of Lucknow
2014-2019
cDNAs coding for a plasma membrane Ca" pump were isolated from human teratoma library and sequenced.The translated sequence contained 1,220 amino acids with calculated molecular weight of 134,683.All regions functional importance known other ion-transporting ATPases could be identified.The also contained, near the carboxyl terminus, calmodulin-binding domain two domains which are very rich in glutamic acid aspartic acid.These resemble calmodulin somewhat one them may play role binding...
Exposure of the purified Ca2+ pump human erythrocytes to chymotrypsin led rapid loss calmodulin activation. A fragment about 12 kDa was removed from ATPase in 1-2 min. Blotting experiments with 125I-labeled showed that this contains binding region. The remainder molecule degraded a number fragments ranging 3 120 kDa; none them bound calmodulin. To isolate domain, which had been coupled Denny-Jaffe reagent (a cleavable radioactive photoaffinity cross-linker) allowed bind pump. After...
The flavivirus genome comprises a single strand of positive-sense RNA, which is translated into polyprotein and cleaved by combination viral host proteases to yield functional proteins. One these, nonstructural protein 3 (NS3), an enzyme with both serine protease NTPase/helicase activities. NS3 plays central role in the life cycle: N-terminal together its essential cofactor NS2B involved processing polyprotein, whereas C-terminal responsible for ATP-dependent RNA separation during...
Metallo-β-lactamases (MBLs) are a growing threat to the use of almost all clinically used β-lactam antibiotics. The identification broad-spectrum MBL inhibitors is hampered by lack suitable screening platform, consisting appropriate substrates and set relevant MBLs. We report procedures for preparation metallo-β-lactamases (i.e., NDM-1 (New Delhi MBL), IMP-1 (Imipenemase), SPM-1 (São Paulo VIM-2 (Verona integron-encoded MBL)) fluorogenic (umbelliferone-derived cephalosporins). were compared...
Second-generation biofuels produced from lignocellulose biomass (LCB) have the potential to compete with fossil fuels, provided that appropriate cost-effective and efficient technologies are used in production process. As agriculture is practiced all over world millions of tons every year, biofuel such wastes could be an environmentally friendly approach meet increasing energy demand. Biofuel quite a challenging process involving several methods need employed for maximum conversion complex...
The plasma membrane Ca2+ pump is a calmodulin-regulated P-type ATPase that an essential element in controlling intracellular concentration. Studies on the gene structure of this have revealed alternate splice option changes calmodulin-binding domain. This change enzyme results reduced calmodulin affinity. Tests enzyme's activity presence high concentration, approximating found inside living cells, show affinity causes apparent for Ca2+. shift activation occurs concentration range crucial to...
The syncytiotrophoblast represents the primary cellular barrier between maternal and fetal circulations in placenta. Large amounts of Ca2+ are transported across this by mechanisms that not clearly understood. To further understand phenomenon, we examined rat human placenta immunohistochemical protein blotting techniques with a monoclonal antibody raised against erythrocyte plasma membrane pump. Immunohistochemistry showed specific staining basal (fetal facing) surface syncytiotrophoblast....
The epitope location and specificity of monoclonal antibodies JA9, 5F10 JA3, raised against the human plasma membrane Ca2+ pump (hPMCA), were analysed by using synthetic peptides corresponding epitopes as well complete isoforms, hPMCA4b, hPMCA4a hPMCA1b, expressed in COS-1 cells. experiments with showed that JA9 reacted specifically a region containing residues 51-75 hPMCA4 (a or b), but not same isoforms 1, 2 3. JA3 1156-1180, unique to hPMCA4b. 719-738, which is highly conserved all PMCA...
ADVERTISEMENT RETURN TO ISSUEPREVArticleMolecular properties of calcium-pumping ATPase from human erythrocytesErnst Graf, Anil K. Verma, Jeffrey P. Gorski, Gary Lopaschuk, Verena Niggli, Mauro Zurini, E. Carafoli, and John T. PennistonCite this: Biochemistry 1982, 21, 18, 4511–4516Publication Date (Print):August 1, 1982Publication History Published online1 May 2002Published inissue 1 August 1982https://pubs.acs.org/doi/10.1021/bi00261a049https://doi.org/10.1021/bi00261a049research-articleACS...
Human calcium transporting tissues were examined to determine whether they contained a protein similar the Ca++-Mg++ adenosine triphosphatase (Ca++-Mg++ATPase) pump of human erythrocyte membrane. Tissues processed for immunoperoxidase staining using monoclonal antibodies against purified Ca++-Mg++ATPase. In kidneys, specific was found only along basolateral membrane distal convoluted tubules. Glomeruli and other segments nephron did not stain. Staining erythrocytes in spleen readily...
In an effort to extend our studies on Ca2+ pumps animal models, we developed a new monoclonal antibody (5F10) prepared against the human erythrocyte Ca2+-Mg2+-adenosinetriphosphatase (ATPase) that recognizes protein of approximately 140 kDa in rat kidney homogenates. Enzyme-linked immunosorbent assays show 5F10 binds purified Ca2+-Mg2+-ATPase and membrane extracts concentration-dependent manner. paraffin-embedded tissue sections, epitope basolateral membranes distal convoluted tubule...
Following the portal vein injection of '261-insulin, radioactivity was taken up by rat liver and concentrated in microsomes (30 to 50% homogenate).Subfractionation revealed that 30 60% label accumulated Golgi elements.Uptake inhibited co-injected unlabeled insulin a dose-dependent manner, desalanine desoctapeptide insulins, proinsulin parallel with their capacities inhibit binding its receptors.Structurally unrelated hormones did not uptake.Maximum labeling plasmalemma heavy, intermediate,...
Plasma membrane fractions from rat corpus luteum contain two kinds of Ca2+-stimulated ATPase, one having a high affinity for Ca2+, the other low Ca2+.The ATPase had specific Ca2+ requirement with K I / Z 0.2 to 0.3 PM; it V , 105 nmol min" mg" and distributed, upon subcellular fractionation, recognized plasma enzymes.The properties this enzyme indicate that is extrusion pump.The pump (KlI2 Caz+, about 15 PM) was nonspecific, being stimulated equally well by or M&+; its function...
Abstract Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator ( Icos ). When absent or mutated (Roquin san ), overexpressed in T cells. Here we show enhances Dicer-mediated processing pre-miR-146a. also directly binds Argonaute2, a central component the RNA-induced silencing complex, miR-146a, microRNA targets mRNA. In absence functional Roquin, miR-146a accumulates Its accumulation not due to...
Although lipid-dependent protein clustering in biomembranes mediates numerous functions, there is little consensus among membrane models on cluster organization or size. Here, we use influenza viral envelope hemagglutinin (HA(0)) to test the hypothesis that results from proteins partitioning into preexisting, fluid-ordered "raft" domains, wherein they have a random distribution. Japan HA(0) expressed fibroblasts was visualized by electron microscopy using immunogold labeling and probed...
The matrix (M) proteins of rhabdoviruses are multifunctional essential for virus maturation and budding that also regulate the expression viral host proteins. We have solved structures M from vesicular stomatitis serotype New Jersey (genus: Vesiculovirus) Lagos bat Lyssavirus), revealing both share a common fold despite sharing no identifiable sequence homology. Strikingly, in stretch residues otherwise-disordered N terminus crystallographically adjacent molecule is observed binding to...