A5013431970- Heart Rate Variability and Autonomic Control
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural dynamics and brain function
- Gene Regulatory Network Analysis
- Neuropeptides and Animal Physiology
- Neuroscience and Neural Engineering
- Bioinformatics and Genomic Networks
- Single-cell and spatial transcriptomics
- Advanced Control Systems Optimization
- Circadian rhythm and melatonin
- Adipose Tissue and Metabolism
- Vagus Nerve Stimulation Research
- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Gene expression and cancer classification
- EEG and Brain-Computer Interfaces
- Stress Responses and Cortisol
- Non-Invasive Vital Sign Monitoring
- MicroRNA in disease regulation
- Microbial Metabolic Engineering and Bioproduction
- Tryptophan and brain disorders
- Neurotransmitter Receptor Influence on Behavior
University of Delaware
2014-2025
Thomas Jefferson University
2016-2025
Bridge University
2021
Jefferson College
2015-2017
Philadelphia University
2015
Jefferson College of Health Sciences
2015
University of Bristol
1997-2011
National Institute of General Medical Sciences
2006
DuPont (United States)
1994-2005
Experimental Station
1986-2005
To study the selectivity of neurotrophic actions in brain, we analyzed several known growth factors on septal cholinergic, pontine and mesencephalic dopaminergic neurons culture. Similar to nerve factor (NGF), basic fibroblast (bFGF) stimulated choline acetyltransferase activity cultures. In contrast NGF, bFGF also enhanced dopamine uptake cultures cell proliferation all 3 culture types. Insulin insulin-like I II transmitter-specific differentiation Epidermal (EGF) produced a small increase...
Although the amygdala complex has long been known to exert a profound influence on cardiovascular activity, neuronal and connectional substrate mediating these influences remains unclear. This paper describes direct amygdaloid projection medullary sensory motor structures involved in regulation, nucleus of solitary tract (NTS) dorsal (DVN), by use autoradiographic anterograde transport retrograde horseradish peroxidase (HRP) techniques rabbits. Since all are highly heterogeneous structurally...
Abstract The intrinsic cardiac nervous system (ICNS), termed as the heart's ‘little brain’, is final point of neural regulation function. Studying dynamic behaviour these ICNS neurons via multiscale neuronal computer models has been limited by sparsity electrophysiological data. We developed and analysed a computational library based on single neuron transcriptomic data obtained from neurons. Each genotype was characterized unique combination ion channels identified data, using cycle...
Uptake, replication, and transneuronal passage of a swine neurotropic herpesvirus (pseudorabies virus, PRV) was evaluated in the rat CNS. PRV localized neural circuits innervating tongue, stomach, esophagus eye with light microscopic immunohistochemistry. In each instance, distribution PRV-immunoreactive neurons entirely consistent that observed following injection cholera toxin-horseradish peroxidase conjugate (CT-HRP). Injections tongue resulted retrograde transport CT-HRP to hypoglossal...
Previous light microscopic work in the rat has demonstrated that many dendrites of vagal gastric motoneurons extend beyond cytoarchitectural boundaries dorsal motor nucleus vagus (DMV) into solitary tract (NST), where they overlap with central terminal field primary sensory neurons (Shapiro and Miselis, 1985a). In present ultrastructural study, synaptic relationship afferent terminals was examined. Following injection cholera toxin-HRP conjugate (CT-HRP) ventral musculature stomach wall,...
Rationale: Cardiac function is under exquisite intrinsic cardiac neural control. Neuroablative techniques to modulate control of are currently being studied in patients, albeit with variable and sometimes deleterious results. Objective: Recognizing the major gaps our understanding control, we sought evaluate regulation impulse initiation sinoatrial node (SAN) as an initial discovery step. Methods Results: We report in-depth, multiscale structural functional characterization innervation SAN...
Gene expression profiles are an increasingly common data source that can yield insights into the functions of cells at a system-wide level. The present work considers limitations in information content gene for reverse engineering regulatory networks. An silico genetic network was constructed this purpose. Using network, formal identifiability analysis performed considered accuracy with which parameters could be estimated using and prior structural knowledge (which transcription factors...
We defined the central circuit innervating various regions of rat heart using a neurotropic herpesvirus as transneuronal tracer. Location viral antigens in brain after cardiac injection three strains pseudorabies virus (PRV) provided insight into vagal preganglionic neurons and their connected interneurons. At short survival times, labeled were localized both nucleus ambiguus (NA) dorsal motor vagus (DMV), an arc-like band through reticular formation between NA DMV. The amount DMV labeling...
The vagus nerve exerts a profound influence on the heart, regulating heart rate and rhythm. An extensive vagal innervation of cardiac ventricles central origin extent this was demonstrated by transynaptic transport pseudorabies virus with virulent two attenuated viral strains. neurons that innervate are numerous, their distribution within nucleus ambiguus dorsal motor is similar to innervating other targets, such as sino-atrial node. These data provide neuroanatomical correlate physiological...
Abstract The source, distribution, and morphology of axons displaying calcitonin gene‐related peptide (CGRP) immunoreactivity in the central amygdaloid nucleus adult rat were investigated with immunohistochemical techniques, both alone combination retrograde transport fluorescent tracers. An extremely dense plexus CGRP‐immunoreactive is differentially concentrated within lateral capsular subdivisions nucleus, much lighter concentrations labeled fibers are present rostral part medial...
1. We explore the roles of conductances in Hodgkin-Huxley (HH) models using a method that allows explicit linking HH model input-output behavior to parameter values for maximal conductances, voltage shifts, and time constants. The procedure can be used identify not only most critical supporting neuronal activity pattern interest but also relationships between parameters which may required, e.g., limited ranges relative magnitudes. 2. is repeated use stochastic search find hundreds or even...
Abstract An understanding of the organization cholinergic neurons in central nervous system has been an important objective for many years. By developing and applying a new electronic method mapping tissue sections, we have generated original graphic quantitative findings on forebrain that provide insight into their distribution organization. Satoh, Armstrong, Fibiger (Brain Res. Bull. 11:693–720, 1983) proposed basal with long axons form continuum rather than being arranged as series...
Drug-seeking in opioid dependence is due part to the severe negative emotion associated with withdrawal syndrome. It well-established that emotional states emerge from activity amygdala. More recently, gut microflora have been shown contribute substantially such emotions. We measured gene expression single glia and neurons gathered amygdala using laser capture microdissection simultaneously morphine-dependent withdrawn rats investigate drivers of withdrawal. found neuroinflammatory genes,...
We have developed and integrated several technologies including whole-organ imaging software development to support an initial precise 3D neuroanatomical mapping molecular phenotyping of the intracardiac nervous system (ICN). While qualitative gross anatomical descriptions anatomy ICN each been pursued, we here bring forth a comprehensive atlas entire rat at single-cell resolution. Our work precisely integrates data in digitally reconstructed whole heart with resolution micron scale. now...
We have developed a bioinformatics tool named PAINT that automates the promoter analysis of given set genes for presence transcription factor binding sites. Based on coincidence regulatory sites, this produces an interaction matrix represents candidate transcriptional network. This currently consists (1) database sequences known or predicted in Ensembl annotated mouse genome database, (2) various modules can retrieve and process sites factors, (3) visualization resulting network connections....
We have used confocal microscopy to analyze the vagal afferent innervation of rat heart. Afferents were labeled by injecting 1,1'-dioleyl-3,3,3',3'-tetramethylindocarbocyanine methanesulfonate (DiI) into nodose ganglia animals with prior supranodose de-efferentations, autonomic stained Fluoro-gold, and tissues examined in whole mounts. Distinctively different fiber specializations observed epi-, myo-, endocardium: epicardium formed complexes associated cardiac ganglia. These consisted four...
Rybak, Ilya A., Julian F. R. Paton, and James S. Schwaber. Modeling neural mechanisms for genesis of respiratory rhythm pattern. II. Network models the central pattern generator. J. Neurophysiol. 77: 2007–2026, 1997. The present paper describes several generator (CRPG) developed employing experimental data current hypotheses rhythmogenesis. Each CRPG model includes a network neuron types (e.g., early inspiratory; ramp late decrementing expiratory; postinspiratory; stage II constant firing...
We injected the anterograde fluorescent tracer 1,1'-dioleyl-3,3,3',3'-tetramethylindocarbocyanine methanesulfonate (DiI) into dorsal motor nucleus of vagus (DmnX), counterstained cardiac ganglia with Fluorogold (FG), and used confocal microscopy to examine distributions different types DmnX fibers in wholemounts atria. also quantified number axons innervated principal neurons (PNs). Rats unilateral DiI injections were three experiments, including FG soaking cervical vagal trunks,...
Abstract We have examined the distribution of neurotensin immunoreactivity within subnuclear regions nucleus tractus solitarius (NTS) and dorsal motor vagus nerve (DVN) in rat. In order to determine which NTS were involved regulation baroreceptor reflexes, we mapped central aortic branch using transganglionic transport horseradish peroxidase. Comparison pattern innervation with that neurotensin‐immunoreactive cells fibers shows dorsomedial both be primary site termination contain highest...
Single-cell heterogeneity confounds efforts to understand how a population of cells organizes into cellular networks that underlie tissue-level function. This complexity is prominent in the mammalian suprachiasmatic nucleus (SCN). Here, individual neurons exhibit remarkable amount asynchronous behavior and transcriptional heterogeneity. However, SCN are able generate precisely coordinated synaptic molecular outputs synchronize body common circadian cycle by organizing networks. To this...
The baroreflex is a multi-input, multi-output control physiological system that regulates blood pressure by modulating nerve activity between the brainstem and heart. Existing computational models of do not explictly incorporate intrinsic cardiac nervous (ICN), which mediates central heart function. We developed model closed-loop cardiovascular integrating network representation ICN within reflex circuits. examined local contributions to rate, ventricular functions, respiratory sinus...