A5032011568- Venomous Animal Envenomation and Studies
- Cardiac electrophysiology and arrhythmias
- Vagus Nerve Stimulation Research
- Nerve injury and regeneration
- Amphibian and Reptile Biology
- Rabies epidemiology and control
- Tissue Engineering and Regenerative Medicine
- Heart Rate Variability and Autonomic Control
- Hemostasis and retained surgical items
- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- Pain Mechanisms and Treatments
- Isotope Analysis in Ecology
- Urinary Bladder and Prostate Research
- Identification and Quantification in Food
- Barrier Structure and Function Studies
- Neurological disorders and treatments
- Pelvic floor disorders treatments
- EEG and Brain-Computer Interfaces
- Spine and Intervertebral Disc Pathology
- Sepsis Diagnosis and Treatment
- Pancreatitis Pathology and Treatment
- Prion Diseases and Protein Misfolding
- Advanced Neuroimaging Techniques and Applications
- Cardiac Arrhythmias and Treatments
Icahn School of Medicine at Mount Sinai
2020-2025
University of California, Los Angeles
2019-2021
Universidade Estadual Paulista (Unesp)
2009-2019
Rationale: Cardiac function is under exquisite intrinsic cardiac neural control. Neuroablative techniques to modulate control of are currently being studied in patients, albeit with variable and sometimes deleterious results. Objective: Recognizing the major gaps our understanding control, we sought evaluate regulation impulse initiation sinoatrial node (SAN) as an initial discovery step. Methods Results: We report in-depth, multiscale structural functional characterization innervation SAN...
Abstract Extensive neovascularization is a hallmark of glioblastoma (GBM). In addition to supplying oxygen and nutrients, vascular endothelial cells provide trophic support GBM via paracrine signaling. Here we report that Endocan ( ESM1 ), an endothelial-secreted proteoglycan, confers enhanced proliferative, migratory, angiogenic properties regulates their spatial identity. Mechanistically, exerts at least part its functions direct binding activation the PDGFRA receptor. Subsequent...
Background: Bone tissue repair remains a challenge in engineering. Currently, new materials are being applied and often integrated with live cells biological scaffolds. The fibrin biopolymer (FBP) proposed this study has hemostatic, sealant, adhesive, scaffolding drug-delivery properties. regenerative potential of an association FBP, biphasic calcium phosphate (BCP) mesenchymal stem (MSCs) was evaluated defects rat femurs. Methods: Adult male Wistar rats were submitted to 5-mm defect the...
Abstract Background The development of new therapeutic strategies to treat amyotrophic lateral sclerosis (ALS) is utmost importance. use cyclic nitroxides such as tempol may provide neuroprotection and improve lifespan. We investigated whether (50 mg/kg) presents potential in SOD1 G93A transgenic mice. Methods Tempol treatment began at the asymptomatic phase disease (10th week) was administered every other day until week 14, after which it twice a final stage disease. animals were sacrificed...
Abstract Axonal characterizations of connectomes in healthy and disease phenotypes are surprisingly incomplete biased because unmyelinated axons, the most prevalent type fibers nervous system, have largely been ignored as their quantitative assessment quickly becomes unmanageable number axons increases. Herein, we introduce first prototype a high-throughput processing pipeline for automated segmentation fibers. Our team has used transmission electron microscopy images vagus pelvic nerves...
Intrinsic cardiac neurons (ICNs) play a crucial role in the proper functioning of heart; yet paucity data pertaining to human ICNs exist. We took multidisciplinary approach complete detailed cellular comparison structure and function from mice, pigs humans. Immunohistochemistry whole sectioned ganglia, transmission electron microscopy, intracellular microelectrode recording dye filling for quantitative morphometry were used define neurophysiology, histochemistry ultrastructure these across...
The vagus nerve provides motor, sensory, and autonomic innervation of multiple organs, electrical stimulation (VNS) an adjunctive treatment option for e.g. medication-refractory epilepsy treatment-resistant depression. mechanisms action VNS are not known, high-resolution anatomical mapping the human is needed to better understand its functional organization. Electron microscopy (EM) required detection both myelinated unmyelinated axons, but access well-preserved nerves ultrastructural...
Intrinsic cardiac neurons (ICNs) play a crucial role in the proper functioning of heart; yet paucity data pertaining to human ICNs exists. We took multidisciplinary approach complete detailed cellular comparison structure and function from mice, pigs, humans. Immunohistochemistry whole sectioned ganglia, transmission electron microscopy, intracellular microelectrode recording dye filling for quantitative morphometry were used define neurophysiology, histochemistry, ultrastructure these cells...
Detection of Leptospira by PCR had not yet been described in snakes. This study investigated, microscopic agglutination test (MAT) and PCR, the presence antibodies to spp. spp., respectively, venomous non-venomous wildlife captivity All snakes were divided into three groups be compared: Group 1 (wildlife - WS); 2 (snakes intensive IC), 3 (collective semi-extensive -CC). Of 147 studied, 52 (35.4%) positive for leptospirosis MAT, 8 (15.4%) belonging (WS), 34 (65.4%) (IC) 10 (19.2%) (CC)....
The venom of Bothrops jararaca is composed complex mixture molecules, mainly lectins, metalloproteinases, serinoproteinases, desintegrins, phospholipases, and peptides. This composition may vary according to the snake's age, gender, region origin. aim was determine individual variation in Botucatu region, Sao Paulo State, Brazil, by means enzymatic, biochemical, pharmacological characterization, utilizing vitro tests biological assays. activities were compared with those Brazilian Reference...
Brachial plexus lesion results in loss of motor and sensory function, being more harmful the neonate. Therefore, this study evaluated neuroprotection regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 Lewis rats were divided into three groups: AX: sciatic axotomy (SNA) without treatment; AX+FS: SNA followed by end-to-end sealant derived from snake venom; AX+CFS: commercial Results analyzed 4, 8, 12 weeks lesion. Astrogliosis, microglial reaction, synapse...
Abstract Objective. Neuromodulation of visceral nerves is being intensively studied for treating a wide range conditions, but effective translation requires increasing the efficacy and predictability neural interface performance. Here we use computational models rat nerve to predict how neuroanatomical variability could affect both electrical stimulation recording with an experimental planar interface. Approach. We developed hybrid pipeline, Vi sceral N erve E nsemble R ecording S timulation...
Abstract TRPV1 is an ion channel that transduces noxious heat and chemical stimuli expressed in small fiber primary sensory neurons represent almost half of skin nerve terminals. Tissue injury inflammation result the sensitization sustained activation can lead to cellular toxicity though calcium influx. To identify signals trigger after a 24-h exposure, we developed phenotypic assay mouse performed unbiased screen with compound library 480 diverse bioactive compounds. Chemotherapeutic...
Abstract Neuromodulation by vagus nerve stimulation (VNS) provides therapeutic benefits in multiple medical conditions, including epilepsy and clinical depression, but underlying mechanisms of action are not well understood. Cervical biopsies were procured from transplant organ donors for high resolution light microscopy (LM) transmission electron (TEM) to map the human fascicular sub-fascicular organization. vagal segments show laterality with right sided dominance fascicle numbers...
Abstract Segmentation of axons in light and electron micrographs allows for quantitative high-resolution analysis nervous tissues, but varied axonal dispersion angles result over-estimates fiber sizes. To overcome this technical challenge, we developed a novel shape-adjusted ellipse (SAE) determination size myelination as an all-inclusive non-biased tool to correct oblique nerve presentations. Our new resource was validated by microscopy against traditional methods determining rhesus...
Abstract Lower urinary tract (LUT) dysfunction is prevalent in the elderly population, and clinical manifestations include retention, incontinence, recurrent infections. Age-associated LUT responsible for significant morbidity, compromised quality of life, rising healthcare costs older adults, but its pathophysiology not well understood. We aimed to investigate effects aging on function by urodynamic studies metabolic markers non-human primates. Adult (n = 27) aged 20) female rhesus macaques...
Abstract Keeping snakes in captivity to produce venom for scientific research and production of inputs is now a worldwide practice. Maintaining involves capture, infrastructure investments, management techniques, appropriate qualified personnel. Further, the success project requires knowledge habitat, nutrition, reproduction, control opportunistic infections. This study evaluated three types (quarantine, intensive, semiextensive) diagnosed bacterial fungal contaminants. A bacteriological...
Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd sacrificed, which causes great harm producer by preventing their exportation ruminant derived-products. Methods: We used stable isotope ratio mass spectrometry (IRMS) carbon (13C/12C) and nitrogen (15N/14N) trace protein blood 15 buffaloes (Bubalus bubalis) divided into three experimental groups: 1 - received...
Rhesus macaques represent an important species for translational and pre-clinical research studies across a multitude of disease injury models, including aging. Ketamine anesthesia is used in humans non-human primates but may be associated with adverse effects, neuromuscular reactions. The effects aging on ketamine not well characterized. Urodynamic recordings electromyography (EMG) were performed aged (>20 years old) adult (3.9–14.9 female rhesus under equal light plane sedation by constant...
Summary CNS neurons do not regenerate after injury, leading to permanent functional deficits. Although sensory and motor neuron axons regrow peripheral nerve outcome is limited due the incomplete slow regrowth. The lack of human-relevant assays suitable for large-scale drug screens has neuro-repair therapy discovery. To address this we developed a phenotypic screening strategy using human induced pluripotent stem cell-derived identify axon-regeneration promoting compounds targets. involve...