A5076320514- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Spinal Cord Injury Research
- Proteoglycans and glycosaminoglycans research
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- Neuroscience and Neural Engineering
- Caveolin-1 and cellular processes
- Advanced biosensing and bioanalysis techniques
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Supramolecular Self-Assembly in Materials
- Lipid Membrane Structure and Behavior
- Cell Image Analysis Techniques
- Sepsis Diagnosis and Treatment
- Inflammation biomarkers and pathways
The University of Texas at Austin
2008-2025
University of California, Los Angeles
2015-2023
Samueli Institute
2021
Broad Center
2017-2021
UCLA Jonsson Comprehensive Cancer Center
2015-2021
Bioengineering Center
2016-2021
UCLA Health
2018-2020
University of Colorado Cancer Center
2019
Center for Special Minimally Invasive and Robotic Surgery
2019
La Jolla Bioengineering Institute
2016-2019
We introduce a novel sensing mechanism for nitric oxide (NO) detection with particular easily synthesized embodiment (NO550), which displays rapid and linear response to NO red-shifted 1500-fold turn-on signal from dark background. Excellent selectivity was observed against other reactive oxygen/nitrogen species, pH, various substances that interfere existing probes. NO550 crosses cell membranes but not nuclear is suitable both intra- extracellular quantifications. Good cytocompatibility...
A major hurdle for regeneration after spinal cord injury (SCI) is the ability of axons to penetrate and grow through scar tissue. After SCI, inflammatory cells, astrocytes meningeal cells all play a role in developing glial scar. In addition, degradation native high molecular weight (MW) hyaluronic acid (HA), component extracellular matrix, has been shown induce activation proliferation astrocytes. However, it not known if HA actually enhances formation. We hypothesize that presence MW (HA...
Abstract Neuromodulation tools are useful to decipher and modulate neural circuitries implicated in functions diseases. Existing electrical chemical cannot offer specific modulation while optogenetics has limitations for deep tissue interfaces, which might be overcome by miniaturized optoelectronic devices the future. Here, a 3D magnetic hyaluronic hydrogel is described that offers noninvasive neuromodulation via magnetomechanical stimulation of primary dorsal root ganglion (DRG) neurons....
Abstract Glioblastoma (GBM) tumors exhibit potentially actionable genetic alterations against which targeted therapies have been effective in treatment of other cancers. However, these largely failed GBM patients. A notable example is kinase inhibitors EGFR, display poor clinical efficacy despite overexpression and/or mutation EGFR >50% GBM. In addressing this issue, preclinical models may be limited by the inability to accurately replicate pathophysiologic interactions cells with...
Abstract Neural stem/progenitor cell (NS/PC)‐based therapies have shown exciting potential for regeneration of the central nervous system (CNS) and NS/PC cultures represent an important resource disease modeling drug screening. However, significant challenges limiting clinical translation remain, such as generating large numbers cells required model or transplantation, maintaining physiologically representative phenotypes ex vivo directing differentiation into specific fates. Here, we report...
In pancreatic ductal adenocarcinoma (PDAC), the abundant stromal cells which comprise tumor microenvironment constitute more than 90% of primary bulk. Moreover, this desmoplastic environment has been found to be three times stiffer normal pancreas tissue. Despite importance studying PDAC, there is still a lack models designed adequately recapitulate complex stiff microenvironment, critical hallmark disease that shown induce chemoresistance. Here, we present bio-mimetic, 3-dimensional...
Increased secretion of hyaluronic acid (HA), a glycosaminoglycan abundant in the brain extracellular matrix (ECM), correlates with worse clinical outcomes for glioblastoma (GBM) patients. GBM cells aggressively invade parenchyma while encountering spatiotemporal changes their local ECM, including HA concentration. To investigate how varying concentrations affect invasion, patient-derived are cultured within soft, 3D which concentration is precisely varied and cell migration observed. Data...
The mechanical properties of solid tumors influence tumor cell phenotype and the ability to invade surrounding tissues. Using bioengineered scaffolds provide a matrix microenvironment for patient-derived glioblastoma (GBM) spheroids, this study demonstrates that soft, brain-like induces GBM cells shift glycolysis-weighted metabolic state, which supports invasive behavior. We first show orthotopic murine are stiffer than peritumoral brain tissues, but stiffness is heterogeneous where edges...
Abstract The development of three‐dimensional, spatially defined neuronal cultures that mimic chemical and physical attributes native tissue is considerable interest for various applications, including the tailored networks clinical repair damaged nerves. Here, use multiphoton excitation to photocrosslink protein microstructures within optically transparent hydrogel materials, such as those based on hyaluronic acid, reported. Multiphoton confines photocrosslinking a three‐dimensional voxel...
Journal Article Sonic hedgehog and neurotrophin-3 increase oligodendrocyte numbers myelination after spinal cord injury Get access Aline M. Thomas, Thomas Department of Biomedical Engineering, McCormick School Northwestern University, Evanston, IL, USA Search for other works by this author on: Oxford Academic Google Scholar Stephanie K. Seidlits, Seidlits Chemical Biological USAInstitute BioNanotechnology in Medicine (IBNAM), Chicago, Ashley G. Goodman, Goodman Todor V. Kukushliev,...
While hyaluronic acid (HA)-based hydrogels have been used clinically for decades, the mechanisms by which HA exerts molecular weight-dependent bioactivity and how chemical modification crosslinking may affect remain poorly understood. This knowledge gap presents a significant barrier to designing with predictable bioactivities. As has widely reported effects on endothelial cells (ECs), we investigated weight of in either soluble or crosslinked forms affects angiogenesis interrogated CD44...
Abstract Extensive neovascularization is a hallmark of glioblastoma (GBM). In addition to supplying oxygen and nutrients, vascular endothelial cells provide trophic support GBM via paracrine signaling. Here we report that Endocan ( ESM1 ), an endothelial-secreted proteoglycan, confers enhanced proliferative, migratory, angiogenic properties regulates their spatial identity. Mechanistically, exerts at least part its functions direct binding activation the PDGFRA receptor. Subsequent...
While in the past hyaluronic acid (HA) was considered a passive component with primarily structural role tissues, research over few decades has revealed its diverse and complex biological functions, resulting major ideological shift. HA is abundant during normal central nervous system (CNS) development and, although down-regulated, remains ubiquitous adult extracellular matrix (ECM). Significant changes content are associated pathological conditions, including stroke, traumatic injury...
Cells are the fundamental functional units of biological systems and mimicking their size, function complexity is a primary goal in development new therapeutic strategies.