Abstract Transcriptional activation of the IL-8 gene by several inflammatory mediators, including cytokines IL-1 and TNF-alpha, is mediated through sequences located between nucleotide -94 -71 promoter. Because adjacent binding sites for inducible transcription factors NF-kappa B NF-IL-6 are within this region, we examined functional interaction these two factor families in regulation. Maximal transcriptional PMA Jurkat T lymphocytes was shown to require intact both NF-IL-6. Electrophoretic...

A straightforward stable isotope dilution analysis (SIDA) for the quantitative determination of trigonelline, nicotinic acid, and nicotinamide in foods such as coffee, well biological samples by means LC-MS/MS (MRM) has been developed. The coefficients variation their a coffee sample were 2.1% 1.1% 3.1% nicotinamide, recovery experiments showed good results between 98.5 104.5%. Application this SIDA quantification different roasting degrees revealed drastic degradation trigonelline...

Epidemiological and experimental evidence increasingly suggests coffee consumption to be correlated prevention or delay of degenerative diseases connected with oxidative cellular stress. In an intervention study comprising 33 healthy volunteers, we examined DNA-protective antioxidative effects exerted in vivo by daily ingestion 750 mL freshly brewed rich both green bean constituents as well roast products. The design encompassed initial 4 wk wash-out, followed intake second wash-out. At the...

Abstract Scope : In order to validate the in vivo function of putatively healthy molecules foods, human intervention studies are required. As subject's compliance concerning intake or abstinence a given food is considered mandatory be monitored by biomarkers, objective was identify analytical markers for coffee consumption. Methods and results Urine samples collected from drinkers were compared with those non‐coffee using hydrophilic liquid interaction chromatography (HILIC)/time‐of‐flight...

Epidemiological studies suggest that coffee can reduce the risk of degenerative diseases such as diabetes type 2, cardiovascular disease and cancer. These beneficial effects have partly been attributed to antioxidant activity coffee. We determined composition potential differentially roasted extracts investigated impact selected original constituents roast products.Parameters studied were direct (trolox equivalent capacity/oxygen radical absorbing capacity), cellular reactive oxygen species...

Abstract Recently, the coffee constituents 5‐ O ‐caffeoylquinic acid (CGA) and N ‐methylpyridinium (NMP) were identified as inducers of Nrf2/antioxidant‐response element (ARE) detoxifying pathway under cell‐culture condition. To study impact CGA NMP on Nrf2‐activating properties a complex beverage, two different model coffees generated by variation roasting conditions: low‐roast rich in heavy‐roast low but containing high levels NMP. Activation was monitored vitro vivo.

Proline betaine has been proposed as a candidate dietary biomarker for citrus intake. To validate its suitability and to gain insight into the range of this per-methylated amino acid in foods beverages, quick accurate stable isotope dilution assay was developed quantitative high-throughput HILIC-MS/MS screening proline urine after solvent-mediated matrix precipitation. Quantitative analysis variety confirmed substantial amounts juices (140-1100 mg/L) revealed high abundance tubers vegetable...

Arabica coffee contains the bitter-tasting diterpene glycoside mozambioside, which degrades during roasting, leading to yet unknown structurally related degradation products with possibly similar bitter-receptor-activating properties. The study aimed at generation, isolation, and structure elucidation of individual pyrolysis mozambioside characterization bitter receptor activation by in vitro analysis HEK 293T-Gα16gust44 cells. new compounds 17-O-β-d-glucosyl-11-hydroxycafestol-2-on,...

The paper reports on the development of an accurate hydrophilic liquid interaction chromatography tandem mass spectrometry (HILIC-MS/MS) based stable isotope dilution analysis for simultaneous quantitation food-derived bioactive pyridines trigonelline, nicotinic acid, nicotinamide, and N-methylpyridinium, as well their key metabolites nicotinamide-N-oxide, N-methylnicotinamide, N-methyl-2-pyridone-5-carboxamide, N-methyl-4-pyridone-5-carboxamide, N-methyl-2-pyridone-5-carboxylic acid in...

Recent results from prospective cohort studies have shown that moderate coffee consumption is associated with a reduced risk for diabetes mellitus type II or Alzheimer's disease. Since reactive oxygen species (ROS) are believed to be involved in the pathogenesis of these diseases, antioxidants might contribute this reduction. We aimed at elucidating whether dark roast beverage (CB) rich N-methylpyridinium ions (NMP: 785 μmol/L) and low chlorogenic acids (CGA: 523 has stronger antioxidant...

This study investigated Nrf2-activating properties of a coffee blend combining raw bean constituents with 5-O-caffeoylquinic acid (CGA) as lead component typical roasting products such N-methylpyridinium (NMP). In cell culture (HT29) the respective extract (CN-CE) increased nuclear Nrf2 translocation and enhanced transcription ARE-dependent genes exemplified for NAD(P)H:quinone oxidoreductase glutathione-S-transferase (GST)A1, reflected in protein level by an increase GST enzyme activity....

Scope Chlorogenic acid (CA), caffeine (CAFF), pyrogallol (PYR), catechol (CAT), β N ‐alkanoyl‐hydroxytryptamides (C5HT) and ‐methylpyridinium (N‐MP) were evaluated for their influence on mechanisms of gastric secretion as single compounds in biomimetic mixtures. Methods results Compounds tested coffee representative concentrations. Human cancer cells (HGT‐1) used to study the proton secretory activity by Ussing chamber experiments FACS analysis. For activation EGFr, Akt1, ERK1/2, ATF‐2 cAMP...

Abstract Pellicle is the initial proteinaceous layer that formed almost instantaneously on all solid surfaces in oral cavity. It of essential relevance for any interactions and metabolism tooth surface. Up to now, there no information metabolome this structure. Accordingly, present study aims characterise metabolomic profile in-situ pellicle children with different caries activity first time comparison saliva. Small molecules such as carbohydrates, amino acids, organic fatty putatively...

Coffee is one of the most consumed hot beverages worldwide and highly regarded because its stimulating effect despite having a pronounced bitterness. Even though numerous bitter ingredients have been identified, detailed molecular basis for coffee's bitterness not well understood except caffeine, which activates five human taste receptors. We elucidated contribution other coffee constituents in addition to caffeine with functional calcium imaging experiments using mammalian cells expressing...

Linseed oil is rich in unsaturated fatty acids, and its increased consumption could aid health-promoting nutrition. However, rapid oxidation of linseed concomitant development bitterness impair consumers' acceptance. Previous research revealed that cyclolinopeptides, a group cyclic peptides inherent to oil, dominantly contribute the observed bitterness. In present study, fresh stored flaxseed were analyzed for presence cyclolinopeptides using preparative high-performance liquid...

Sensory-guided fractionation of a roasted coffee beverage revealed highly polar, bitter-tasting subfraction, from which the furokaurane glucoside mozambioside was isolated and identified in its chemical structure by means HDMS NMR spectra. Sensory evaluation bitter taste recognition threshold 60 (± 10) μmol/L. UPLC-HDMS quantitation raw beans showed that Arabica coffees contained 396-1188 nmol/g mozambioside, whereas only traces (<5 nmol/g) were detected Robusta coffees, thus suggesting can...

Quantitative analysis of the bioactives trigonelline (1), N-methylpyridinium (2), caffeine (3), and caffeoylquinic acids (4) in a large set roasted Arabica (total sample size n = 113) Robusta coffees 38) revealed that concentrations 1 4 significantly correlated with roasting color (P < 0.001, two tailed), whereas 2 inversely tailed). As dark-roasted were rich light-roasted acids, manufacturing roast all four would therefore necessitate blending or even more different colors. Additional...

The objective of this study was to investigate the potential various raw bean components as precursors pyrogallol (1), hydroxyhydroquinone (2), catechol (3), 4-ethylcatechol (4), 4-methylcatechol (5), and 3-methylcatechol (6) under quasi "natural" roasting conditions by using recently developed "in bean" model roast experiments. Freeze-dried, fully extracted shells were loaded with aqueous solutions either single coffee compounds or fractions isolated from solubles. After freeze-drying,...

As the consumption of coffee beverages sometimes is reported to cause gastric irritation, for which an increased stomach acid secretion one promoting factors, different processing technologies such as steam-treatment have been developed reduce putative irritating compounds. There evidence-based data neither on effect detailed variations nor individual components affecting proton secretory activity (PSA). This work aimed at developing a screening model suitable investigating effects...