Summary We explore by extensive mutagenesis regions in the sequence allowing reversal of allosteric response Tet repressor. The wild type requires anhydrotetracycline for induction. About 100 mutants are presented, which, contrast, require drug repression. Their mutations clustered at interface DNA‐ and inducer‐binding domains. This consists a central hydrophobic region surrounded several hydrogen bonds. While most described here contain two to five mutations, we found positions this TetR,...

Abstract This study aimed to evaluate the effect of self-assembling peptide P 11 -4 (SAP) in therapy initial smooth surface caries (white spot lesions, WSL) following orthodontic multibracket treatment. Twenty-three patients (13f/10m; average age 15.4 years) with at least two teeth WSL were recruited for randomised controlled clinical trial split-mouth design. In opposite control teeth, test treated SAP on Day 0. The primary endpoint was impedance measurement using customised tray ensure...

Abstract Pellicle is the initial proteinaceous layer that formed almost instantaneously on all solid surfaces in oral cavity. It of essential relevance for any interactions and metabolism tooth surface. Up to now, there no information metabolome this structure. Accordingly, present study aims characterise metabolomic profile in-situ pellicle children with different caries activity first time comparison saliva. Small molecules such as carbohydrates, amino acids, organic fatty putatively...

The first step of bioadhesion on dental surfaces is the formation acquired pellicle. This mainly acellular layer formed instantaneously all solid exposed to oral fluids. It composed proteins, glycoproteins and lipids. However, information lipid composition sparse. aim present study was evaluate fatty acid (FA) profile in-situ pellicle for time. Furthermore, impact rinses with safflower oil pellicle's FA investigated. Pellicles were in situ bovine enamel slabs mounted individual upper jaw...

To avoid adverse side effects of chemicals, pharmaceuticals, and their transformation products (TPs) in the environment, substances should be designed to fully mineralize environment at end-of-life while ensuring a degree stability as needed for application. These considerations implemented very beginning chemical's pharmaceutical's design (Benign by Design, BbD) meet requirements set planetary boundaries upcoming legal frameworks (e.g., "Chemicals Strategy Sustainability towards Toxic-Free...

Abstract UV filters and benzotriazole stabilizers are considered emerging contaminants in the environment. LC–MS/MS GC–MS methods, involving a single solid phase extraction protocol, were developed validated to determine eight seven stabilizers, respectively wastewater from treatment plant (WWTP) Lüneburg, Germany. The method exhibited recoveries of ≥ 71% at six different fortification levels with limits detection (LODs) range 0.02 ng mL −1 – 0.09 . Extraction 47 119% obtained for LODs 0.01...

Within the past years MALDI-TOF mass spectrometry has become a powerful tool for species identification of cultured bacteria and fungi.In present study, implementation in commercial high throughput laboratory is described.The impact different growth conditions on results was evaluated.Although slight differences spectra E. coli S. aureus strains blood agar various periods (5, 18, 24 48 hours) were noticed, reliable obtained all periods.The same true when 18 hours solid media.Reliable also...

The acquired pellicle is a tenacious organic layer covering the surface of teeth, protecting underlying dental hard tissues. Lipids account for about one quarter pellicle's dry weight and are assumed to be considerable importance their protective properties. Nevertheless, only preliminary information available nature lipids in pellicle. Gas chromatography coupled with electron impact ionization mass spectrometry was used establish convenient analytical protocol order obtain qualitative...

The combined impact of temperature and light spectra on the fatty acid (FA) composition in microalgae has been sparsely investigated. aim this study was to investigate interactions FA Acutodesmus obliquus. For purpose, A. obliquus cultivated with different temperatures (20, 30, 35°C), as well broad (blue, green, red light). Growth were monitored daily. Microalgal extracted, a qualitative characterization done by gas chromatography coupled electron ionization mass spectrometry (GC-EI/MS)....

Abstract Blue‐green light is known to maximize the degree of fatty acid (FA) unsaturation in microalgae. However, knowledge on particular waveband responsible for this stimulation FA desaturation and its impact pigment composition microalgae remains limited. In study, Acutodesmus obliquus was cultivated 96 h at 15°C with different spectra (380–700 nm, 470–700 520–700 600–700 dark controls). Growth monitored daily, qualitative characterization microalgal achieved via gas chromatography...

Lipids are of interest for the targeted modification oral bioadhesion processes. Therefore, sustainable effects linseed oil on composition and ultrastructure in situ pellicle were investigated. Unlike saliva, contains linolenic acid (18:3), which served as a marker lipid accumulation. Individual splints with bovine enamel slabs worn by five subjects. After 1 min formation, rinses performed 10 min, slabs’ exposure was continued up to 2 or 8 h. Gas chromatography coupled electron impact...

Abstract The spectral composition of light is an important factor for the metabolism photosynthetic organisms. Several blue light‐regulated metabolic processes have already been identified in industrially relevant microalga Monoraphidium braunii . However, little known about impact on this species' growth, fatty acid (FA), and pigment composition. In study, M. was cultivated under different spectra (white light: 400–700 nm, 400–550 green 450–600 red 580–700 nm) at 25°C 96 h. growth monitored...