Alkaloid-enriched extract of Huperzia serrata (Thunb.) Trevis (Lycopodiaceae) (HsAE) can potentially be used to manage neuronal disorders.This study determines the anti-neuroinflammatory effects HsAE on lipopolysaccharide (LPS)-stimulated BV-2 microglial cells and underlying mechanisms.BV-2 were pre- or post-treated with different concentrations (25-150 µg/mL) for 30 min before after LPS induction. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium...

To evaluate the antitumor activity both in vitro and vivo of saponin–phospholipid complex Panax notoginseng. The cytotoxic effect saponins extract against human lung cancer NCI-H460 breast cell lines BT474 was examined using MTS assay. For evaluation potential, saponin were administered orally rats induced mammary carcinogenesis by 7,12-dimethylbenz(a)anthracene, for 30 days. Our data showed that had stronger anticancer compared to extract. IC50 values 28.47 μg/mL 47.97 μg/mL, respectively...

Abstract Background Zingiber officinale Roscoe has been used in traditional medicine for the treatment of neurological disorder. This study aimed to investigate phenolic contents, antioxidant, acetylcholinesterase enzyme (AChE) inhibitory activities different fraction Z. root grown Vietnam. Methods The roots are extracted with ethanol 96 % and fractionated n-hexane, ethyl acetate (EtOAc) butanol (BuOH) solvents. These fractions evaluated antioxidant activity by 1,1-Diphenyl -2-picrylhydrazyl...

To investigated the protective potential of ethanol extracts Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Dried roots S. were extracted with and concentrated to yield a dry residue. Mice administered 200 mg/kg orally once daily for one week. Animals subsequently single dose LPS (5 body weight, intraperitoneal injection). Both protein mRNA levels cytokines, such as tumor necrosis factor alpha, interleukin-1β, interleukin-6 in tissues...

Abstract Huperzia serrata contains Huperzine A (HupA)—an alkaloid used to treat cognitive dysfunction. In this study, we the total alkaloids (HsAE) investigate their potential in managing impairment comparison with HupA. The antioxidant activity was measured by DPPH assay. cellular cell viability and level of ACh SH‐SY5Y cells were evaluated after pretreated HsAE scopolamine. For vivo assay, mice pre‐treated HsAE, HupA undergone scopolamine injection for impairment. behavioral tests...

Abstract The present study investigated the phytochemicals and antidiabetic effect of aqueous extract fruit Punica granatum Linn. in streptozotocin (STZ)-induced diabetic mice. P. was extracted with water. water were by liquid chromatography–tandem mass spectrometry (LC-MS/MS) method. at doses 150 300 mg/kg body weight (bw) administered to mice for 21 days, blood glucose level, triglyceride, total cholesterol, low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein-cholesterol,...

Alzheimer's disease (AD) is a lingering progressive neurodegenerative disorder that causes patients to lose cognitive function. The enzyme Acetylcholinesterase (AChE), Butyrylcholinesterase (BuChE), Monoamine oxidase A (MAO A), Beta-secretase cleavage (BACE 1) and N-methyl-D-aspartate (NMDA) receptors play an important role in the pathogenesis of disease. Therefore, inhibiting enzymes effective method treat Alzheimer In this study, we evaluated silico inhibitory effects AChE, BuChE, MAO A,...

Pharmacognosy Research,2019,11,4,396-399.DOI:10.4103/pr.pr_82_19Published:November,2019Type:Original ArticleAuthors:Dang Kim Thu, Dao Thi Vui, and Bui Thanh Tung Author(s) affiliations:Dang Thu1, Vui2, Tung1 1 Department of Pharmacology Clinical Pharmacy, VNU School Medicine Vietnam National University, Hanoi, Vietnam. 2 Pharmacology, Hanoi University Abstract:Background: Huperzia phlegmaria has been used to enhancing memory alleviate brain disorders. It contains high amount alkaloids, which...

Abstract: α-glucosidase enzyme is one of the important molecular targets in treatment diabetes. In addition, free radicals are cause insulin resistance, damage β- cell pancreatic, glucose uptake disorders and induced this study we evaluated inhibitory effect antioxidant by using DPPH radical scavenging method green coffee bean extract (Coffea canephora) its fractions. Coffee beans were pulverized, extracted with ethanol 70% sonications, fractionated n-hexane, ethyl acetate (EtOAc) n-butanol...

Xanthine oxidase (XO) is an enzyme that has improtant role in the synthesis of uric acid. XO allowscatalyzing hydroxylation hypoxanthine to xanthine and These are two reactions final stage metabolism purines body. Offal, inhibitors reduce biosynthesis acid have been used prevent treat gout. In this study, Gompherena celosiodes extracted by ultrasonic with ethanol 80 % (EtOH)solvents fractionated n-hexane, ethyl acetate (EtOAc) n-butanol (n-BuOH) solvents. fractions were evaluated inhibitory...

Acetylcholinesterase (AChE) is a key target in the treatment of Alzheimer’s disease. Principal role AChE hydrolyzes neurotransmiter acetylcholine. Medicinal plants are potential source inhibitors. In this study, we studied inhibitory activities extraction Mahonia nepalensis. This medicianl plant was extracted with ethanol 96% and subsequently fractionated n-hexane, ethyl acetate (EtOA) n-butanol (n-BuOH) solvents. These fractions were evaluated activity by Ellman’s colorimetric method....

Introduction: Sarcandra glabra has been used in traditional medicine to improve mental efficiency and reduce stress. Volatile oil, phenolic acids, polysaccharide, terpenoids, saponins flavonoids are bioactive compounds present glabra. Materials Methods: In this paper, we evaluated the antioxidant capacity acetylcholinesterase (AChE) inhibitory activity vitro of different fractions ethanolic extract neuroprotective effects EtOAc fraction on cognitive deficits mice induced with scopolamine....

Medicinal plants are a potential source of enzyme acetylcholinesrerase (AChE) inhibitors, key target in the treatment Alzheimer’s disease. This paper studies AChE inhibitory activity and antioxidant effect Persea Americana Mill extract. The sample leave, seed, exocarp mesocarp avocado were extracted with 50% ethanol subsequently fractionated n-hexane, ethyl acetate (EtOA) n-butanol (n-BuOH) solvents. was evaluated by Ellman’s colorimetric method screening DPPH free radicals. results show...

Herbal extract, rich with natural compounds, has been used for medicinal purpose such as treating neurological disorders cognitive defection a long period of time, often without significant adverse effects. We compared AChE and BuChE – inhibition effect total extracts fractions Huperzia serrata (Thunb.) Trevis. alcaloid-rich extract. Our samples were subjected under supersonic extraction ethanol 50o solvent fractionally extracted n-hexane, EtOAc n-butanol, respectively; extract was collected...

Background: Huperzia phlegmaria has been used for the treatment of neurological disorder. Alkaloids are main bioactive compounds found in phlegmaria. We aimed to investigate acetylcholinesterase (AChE) inhibitory activity vitro alkaloid extract (HpAE) and protective effects on mice which were induced cognitive deficits by scopolamine. Methods: AChE kinetic inhibition mechanism was investigated Ellman's assay. Mice administrated orally HpAE (30 mg/kg 60 mg/kg) fourteen days, injected...

The group of protein drugs has been developing very strongly, promising great advances in diagnosis, treatment, prevention, and human health improvement. Protein drug production is a large area research, requiring advanced scientific technological expertise, integration multi-disciplinary technology from fields such as biology, genetics, biotechnology, extraction purification, pharmaceutical manufacturing.
 As manufacture involves biosynthesis processes using different cell lines,...

This study aimed to investigate the acute toxicity, analgesic and anti-inflammatory effects of hard capsules containing dry extract from Siegesbeckia orientalis L. (CKHT) in animal models. Hard were prepared using volumetric method, with Avicel PH 102 as diluent excipient magnesium carbonate adsorbent excipient. The toxicity CKHT was assessed on white mice at a dose 2,400 mg/kg body weight. effect evaluated carrageenan-induced paw edema model rats, while tested an acetic acid-induced...

Type 2 diabetes is a fairly common chronic disease. α-glucosidase and protein tyrosine phosphatase, as enzymes, play an important role in type diabetes. This study evaluates the inhibitory effect of two enzymes vitro ethanol extract fractions Vietnam Psidium guajava’s leaves. The leaves were collected, dried extracted with 96% successively fractionated n-hexane, ethyl acetate butanol solvents. results show that EtOH extract, n-nexan, EtOAc BuOH had high IC50 values ​​of 2.20; 2.53; 2.24 2.16...

Introduction: Overactivated microglia are a chronic source of meaningful neurotoxic factors, and the inhibition microglial overactivation restores neuronal survival. Therefore, downregulation activation may possibly slow or delay progression neurodegenerative disorders. Huperzia serrata (Thunb.) Trevis has been widely used to treat diseases involving neuromuscular cardiovascular diseases. However, anti-neuroinflammatory effects alkaloid-enriched extracts H. (HsAE) have not yet investigated....

A liquid chromatography-electrospray ionization tandem mass spectrometry in a positive mode (LC-ESI-MS/MS) was developed to determine simultaneously the levels of cortisol and testosterone rat serum. The MRM (Multiple Reaction Monitoring) used optimize ion precursor, fragmentor voltage, product, collision energy. Analytical samples were treated by solid-phase extraction with Bond Elut C18 cartridge. method validated according FDA guidance for bioanalytical validation. results indicated that...

Background: HER2-positive breast cancer is a that tests positive with human epidermal growth factor receptor 2 (HER2). Human Epidermal Growth Factor Receptor-2 (HER2) promotes the proliferation of cancers cells. This research aimed to find bioactive compounds from Allium sativum for inhibiting HER2 enzyme by using molecular docking method. Materials and method: The protein tyrosin kinase structure was obtained Protein Data Bank. Compounds were collected previous publications these structures...