A5029705489- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Mathematical and Theoretical Epidemiology and Ecology Models
- Evolution and Genetic Dynamics
- Gene Regulatory Network Analysis
- Hepatitis C virus research
- Mathematical Biology Tumor Growth
- Cancer Immunotherapy and Biomarkers
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- vaccines and immunoinformatics approaches
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Tuberculosis Research and Epidemiology
- Viral Infections and Immunology Research
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis B Virus Studies
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
Universitat Pompeu Fabra
2015-2024
Institució Catalana de Recerca i Estudis Avançats
2015-2024
Russian Academy of Sciences
2016-2020
Institute of Numerical Mathematics
2016-2020
Biology of Infection
2013
Saarland University
2003-2012
Institute of Virology of the Slovak Academy of Sciences
2012
Helmholtz Institute for Pharmaceutical Research Saarland
2012
Barcelona Biomedical Research Park
2010
Institute of Medical Microbiology and Hygiene
1992-2007
Journal Article DNA recombination during PCR Get access Andreas Meyerhans, Meyerhans Laboratoire de Biologie et Immunologie Moléculaires des Rétrovirus, Institut Pasteur28 Rue du Docteur Roux, 75724 Paris Cedex 15, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Jean-Pierre Vartanian, Vartanian Simon Wain-Hobson * To whom correspondence should be addressed Nucleic Acids Research, Volume 18, Issue 7, 11 April 1990, Pages 1687–1691,...
Human immunodeficiency virus type 1 (HIV-1) isolates are genetically so heterogeneous that they must be described in terms of populations related but distinct genomes called quasispecies. A recent study the influence ex vivo culturing on HIV-1 quasispecies demonstrated usually low-abundance outgrew more prominent forms. Here it is shown multiple passages an isolate peripheral blood mononuclear cells resulted outgrowth very minor single passage equal proportions supernatants to either...
Background. Immunosuppressive treatment in transplant patients frequently causes infectious complications with cytomegalovirus (CMV). The extent of CMV replication can be followed by a number diagnostic methods. There is, however, no simple tool to assess the quality cellular antiviral immune response an individual patient. This would particular importance for therapy decisions, as detectable virus load do not necessarily develop CMV-related disease. Using rapid whole blood assay,...
The acid-fast bacillus Mycobacterium tuberculosis is often the first manifestation of acquired immunodeficiency syndrome in patients infected with human virus (HIV). This study was conducted to better understand mechanism underlying M. tuberculosis-specific pathogenicity early after onset HIV infection.M. T helper 1 (Th1) cells were studied negative (n=114) and chronically (n=68) Tanzanian subjects by using secreted antigenic target 6 (ESAT6) protein or tuberculin (purified derivative)...
The importance of HLA class I-restricted CD8 T-cell responses in the control human immunodeficiency virus (HIV) infection is generally accepted. While several studies have shown an association certain I alleles with slower disease progression, it not fully established whether this effect mediated by HIV-specific restricted these alleles. In order to study influence on response and viral control, we assessed epitope recognition, plasma load, expression a cohort HIV-seropositive bar workers....
Human APOBEC3 cytidine deaminases target and edit single-stranded DNA, which can be of viral, mitochondrial, or nuclear origin. Retrovirus genomes, such as human immunodeficiency virus (HIV) genomes deficient in the vif gene hepatitis B genome, are particularly vulnerable. The some DNA viruses, papillomaviruses, edited vivo transfection experiments. Accordingly, herpesviruses should no exception. This is indeed case for herpes simplex 1 (HSV-1) tissue culture, where APOBEC3C (A3C)...
Inevitably, viruses depend on host factors for their multiplication. Here, we show that hepatitis C virus (HCV) RNA translation and replication depends Rck/p54, LSm1, PatL1, which regulate the fate of cellular mRNAs from to degradation in 5'-3'-deadenylation-dependent mRNA decay pathway. The requirement these proteins efficient HCV was linked 5' 3' untranslated regions (UTRs) viral genome. Furthermore, LSm1-7 complexes specifically interacted with essential cis-acting elements located UTRs....
Significance Human DEAD-box polypeptide 3 (DDX3) is an ATPase/RNA helicase involved in the replication of many viral pathogens. We reported herein first inhibitor, to our knowledge, binding site DDX3 endowed with a broad spectrum antiviral activity [HIV-1 WT, HIV drug-resistant strains, Hepatitis C virus (HCV), Dengue (DENV), and West Nile (WNV)]. The good toxicity profile suggests that activity, although essential for viruses, could be dispensable cells, validating as pharmaceutical target....
The quasispecies model for RNA viruses predicts the existence of a replication error threshold beyond which there is melting or total loss sequence information. Retroviral G-->A hypermutation probably an example. Here it shown that transitions may occur in both GpG and GpA dinucleotide contexts. Transitions preferentially via base mispairing at ends runs G residues, whereas within result from temporary dislocation primer template strands by single base. two circumstances be related local...
Human immunodeficiency virus type 1 (HIV-1) replication is shown to be sensitive the intracellular concentration of deoxynucleoside triphosphate substrates. Addition thymidine established cell lines resulted in a dramatic reduction production. The effect could substantially alleviated by addition deoxycytidine, which, alone, enhanced viral titers factor 2 3. Hydroxyurea treatment abolished HIV-1 peripheral blood mononuclear cells and reversed deoxyadenosine. These data show that occurs under...
Investigations on pulmonary macrophages (MΦ) mostly focus alveolar MΦ (AM) as a well-defined cell population. Characteristics of in the interstitium, referred to lung interstitial (IM), are rather ill-defined. In this study we therefore aimed elucidate differences between AM and IM obtained from human tissue. Human were isolated non-tumor tissue patients undergoing resection. Cell morphology was visualized using either light, electron or confocal microscopy. Phagocytic activity analyzed by...
<h3>Importance</h3> Therapies targeting the programmed cell death 1 (PD-1) receptor or its ligand (PD-L1), such as humanized monoclonal antibody durvalumab, have shown durable clinical responses in several tumor types. However, concerns about safety and feasibility of PD-1/PD-L1 blockade HIV-1–infected individuals led to exclusion these patients from trials on cancer immunotherapies. <h3>Objective</h3> To evaluate durvalumab treatment with advanced virologically controlled HIV-1 infection....
Current COVID-19 vaccines have been associated with a decline in infection rates, prevention of severe disease, and decrease mortality rates. However, SARS-CoV-2 variants are continuously evolving, development new accessible is essential to mitigate the pandemic. Here, we present data on preclinical studies mice receptor-binding domain (RBD)-based recombinant protein vaccine (PHH-1V) consisting an RBD fusion heterodimer comprising B.1.351 B.1.1.7 formulated SQBA adjuvant, oil-in-water...
Abstract Vaccines based on mRNA technology have revolutionized the field. In fact, lipid nanoparticles (LNP) formulated with are preferential vaccine platform used in fight against SARS-CoV-2 infection, wider application other diseases. The high demand and property right protection of most potent cationic/ionizable lipids for LNP formulation COVID-19 vaccines promoted design alternative nanocarriers nucleic acid delivery. this study we evaluated immunogenicity efficacy different rationally...
ABSTRACT Replication of cytomegalovirus (CMV) is largely controlled by the cellular arm immune response. In this study CMV-specific CD4 T-cell response was characterized in a cohort apparently healthy individuals. 11% all individuals, extremely high frequencies, between 10 and 40%, were found. High-level frequencies T cells persisted over several months not result an acute infection. Specific oligoclonal phenotypically functionally as mature effector cells, with both cytokine-secreting...
Abstract The vigorous CTL response directed against HIV is considered to be important in reducing viral load, although it unable stop ongoing replication, which generates new antigenic variants. We analyzed the impact of sequential changes five epitopes HIV-1 Nef on recognition four stable patients. A high rate variation was found, and all these patients we could detect specific for 32 out 36 autologous variants occurring 5 HLA-A2- or HLA-B7-restricted at two time points. Two distinct...