A5049602650- Salivary Gland Disorders and Functions
- Oral microbiology and periodontitis research
- Cynara cardunculus studies
- Advanced Proteomics Techniques and Applications
- Microbial Metabolites in Food Biotechnology
- Oral and gingival health research
- Machine Learning in Bioinformatics
- Vitamin K Research Studies
- Enzyme Catalysis and Immobilization
- Protease and Inhibitor Mechanisms
- Biochemical and Molecular Research
- Plant biochemistry and biosynthesis
- Dental Implant Techniques and Outcomes
- Glycosylation and Glycoproteins Research
- Botanical Studies and Applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Carbohydrate Chemistry and Synthesis
- Identification and Quantification in Food
- Enzyme Structure and Function
- Lysosomal Storage Disorders Research
- Enzyme Production and Characterization
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
Universidade Católica Portuguesa
2015-2024
University of Lisbon
2016
University of Coimbra
1992-2013
Association for Innovation and Biomedical Research on Light and Image
2009
University of Aveiro
1998-2006
Complejo Hospitalario de Salamanca
2006
SARS-CoV-2 pandemic has forced frequent testing of populations. It is necessary to identify the most cost-effective strategies for detection COVID-19 outbreaks. Nasopharyngeal samples have been used but require a healthcare professional collect sample and cause discomfort pain individual. Saliva suggested as an appropriate fluid diagnosis COVID-19. We investigated possibility using pools saliva detect in symptomatic asymptomatic patients. Two hundred seventy-nine were analyzed through RT-PCR...
Abstract Background The oral cavity is a complex ecosystem where human chemical compounds coexist with particular microbiota. However, shifts in the normal composition of this microbiota may result onset ailments, such as periodontitis and dental caries. In addition, it known that microbial colonization mediated by protein-protein interactions (PPIs) between host microorganisms. Nevertheless, kind PPIs still largely undisclosed. To elucidate these interactions, we have created computational...
Biobank saliva sample quality depends on specific criteria applied to collection, processing, and storage. In spite of the growing interest in as a diagnostic fluid, few biobanks currently store large collections such samples. The development standard operating procedure (SOP) for collection control is fundamental establishment new biobank, which stores samples be made available research community. Different methods were tested regarding total volume protein obtained, content, profiles,...
Identifying ZIKV factors interfering with human host pathways represents a major challenge in understanding tropism and pathogenesis. The integration of proteomic, gene expression Protein-Protein Interactions (PPIs) established between proteins predicted by the OralInt algorithm identified 1898 interactions medium or high score (≥0.7). Targets implicated vesicular traffic docking were identified. New receptors involved endocytosis as entry targets, using both clathrin-dependent (17...
COVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 deaths worldwide, in addition to an unprecedented economic impact. Despite many advances scientific knowledge about disease, much remains be clarified molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics silico interactomics strategy establish salivary protein profile. Data are available via ProteomeXchange identifier PXD036571. The differential...
Surface treatments are designed to promote modified implant surfaces with positive interactions the surrounding living tissues. However, inadvertent early contact of these oral fluids during surgery may lead undesired conditions affecting osseointegration. This study aimed investigate possible alterations in physico-chemical properties modified-surfaces caused by saliva exposure. Titanium (Ti) were exposed three different samples human and later analyzed for protein adhesion, surface...
Cardoon flower extract is a traditional and exclusive rennet used for some PDO cheeses in several Mediterranean regions, due to its extremely high concentration cardosins. In this preliminary study, six individual cardoon genotypes (1M – 6M) were selected because they revealed wide consistent diversity of total specific cardosin concentrations flowers. During three growing seasons, the stability 12 biochemical characteristics extracts 26 plant morphological descriptors was confirmed....
Abstract Aims GBR membranes have various surface properties designed to elicit positive responses in regenerative clinical procedures; dental clinicians attempt employ techniques prevent the direct interaction of contaminated oral fluids with these biomaterials. However, saliva is uninterruptedly exhibited surgical procedures applying membranes, suggesting a persistent biomaterials and surrounding tissues. This fundamental study aimed investigate potential alterations physical, chemical, key...
Cardosin A is extracted from the pistils of plant Cynara cardunculus L. and chitosan a polysaccharide derived chitin with valuable properties as biomaterial. In this work we report our experiments on synthesis sponges immobilisation cardosin A, by entrapment. We observed that 10-15% incorporated were released over 6 days incubation. addition, could also note procedure did not induce any specificity alterations A. The study enzyme, using beta-chain oxidised insulin, showed immobilised enzymes...