A5063817372- Acute Ischemic Stroke Management
- Traumatic Brain Injury and Neurovascular Disturbances
- Intracranial Aneurysms: Treatment and Complications
- Cerebrovascular and Carotid Artery Diseases
- Blood properties and coagulation
- Protease and Inhibitor Mechanisms
- Neurosurgical Procedures and Complications
- Allelopathy and phytotoxic interactions
- Seed Germination and Physiology
- Heat shock proteins research
- Cassava research and cyanide
- Plant responses to water stress
- Plant tissue culture and regeneration
- Innovative Microfluidic and Catalytic Techniques Innovation
- Monoclonal and Polyclonal Antibodies Research
- Vascular Malformations Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Wheat and Barley Genetics and Pathology
- Advanced Biosensing Techniques and Applications
- Plant Stress Responses and Tolerance
- 3D Printing in Biomedical Research
- Venous Thromboembolism Diagnosis and Management
- Advanced Proteomics Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
St. Anne's University Hospital Brno
2024
University Hospital Brno
2024
Masaryk University
2023-2024
Veterinary Research Institute
2019-2020
Palacký University Olomouc
2013
Mendel University in Brno
2007-2009
Changes in Hsp70 gene expression and protein level were studied three Solanum spp. genotypes response to short-term high low temperatures infection by powdery mildew. Development of Oidium neolycopersici was compared on plant leaves leaf discs with regard the influence temperature. Heat especially cold pre-treatment host tissues inhibited pathogenesis decreased chlorophyll concentration. Exposure heat increased (70 kDa shock proteins) content all spp., whereas induced accumulation only...
Development of tools for direct thrombus imaging represents a key step diagnosis and treatment stroke. Nanoliposomal carriers contrast agents thrombolytics can be functionalized to target blood thrombi by small protein binders with selectivity fibrin domains uniquely formed on insoluble fibrin. We employed highly complex combinatorial library derived from scaffold 46 amino acid albumin-binding domain (ABD) streptococcal G, ribosome display, identify variants recognizing cloth in human...
Intravenous thrombolysis with a recombinant tissue plasminogen activator (rt-PA) is the first-line treatment of acute ischemic stroke. However, successful recanalization relatively low and underlying processes are not completely understood. The goal was to provide insights into clinically important factors potentially limiting rt-PA efficacy such as clot size, concentration, age also in combination heparin anticoagulant. We established static vitro thrombolytic model based on red blood cell...
The interplay between nitric oxide (NO) and reactive oxygen species can lead to an induction of cell death in plants. aim our work was find out if cyanide released from sodium nitroprusside (SNP; a donor NO) could be involved the induction, which is triggered by SNP H2O2. Cell suspension Nicotiana tabacum L. (line BY-2) treated with 0.5 mM SNP, potassium ferricyanide (PFC; analogue not release and/or glucose U cm-3 oxidase (GGO; system H2O2). induced only combination GGO. Thus death....
On-chip vascular microfluidic models provide a great tool to study aspects of cardiovascular diseases in vitro. To produce such models, polydimethylsiloxane (PDMS) has been the most widely used material. For biological applications, its hydrophobic surface be modified. The major approach plasma-based oxidation, which very challenging case channels enclosed within chip. preparation chip combined 3D-printed mold with soft lithography and commonly available materials. We have introduced...
Background Understanding the risk factors leading to intracranial aneurysm (IA) rupture have still not been fully clarified. They are vital for proper medical guidance of patients harboring unruptured IAs. Clarifying hemodynamics associated with point could help provide useful information about some factors. Thus far, few studies studied this issue often diverging conclusions. Methods We identified a in operated an IAs during surgery, using combination preoperative computed tomography (CT)...
Collaterals improve recanalization in acute ischemic stroke patients treated with intravenous thrombolysis, but the mechanisms are poorly understood. To investigate it, an vitro flow model of middle cerebral artery was developed or without collaterals. An occlusion achieved using human blood clots. Recanalization time, thrombolysis (clot length decrease and red cell (RBC) release), pressure gradient across clot compaction were measured. Results showed that collateral alteplase-treated RBC...
Diseases with the highest burden for society such as stroke, myocardial infarction, pulmonary embolism, and others are due to blood clots. Preclinical clinical techniques study clots important tools translational research of new diagnostic therapeutic modalities that target In this study, we employed a three-dimensional (3D) printed middle cerebral artery model image under flow conditions using preclinical imaging including fluorescent whole-body imaging, magnetic resonance (MRI), computed...
Introduction: Direct clot targeting represents attractive concept for imaging as well targeted delivery of drugs, e.g. thrombolytics. Small protein binders attached to nanoliposomes may target thrombi and deliver drugs although selective affinity fibrin not fibrinogen is the main challenge. Methods: For identification preparation fibrin-specific artificial derived from scaffolds albumin-binding domain (ABD) streptococcal G, a highly complex ABD-derived combinatorial library in combination...