A5013044144- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Fermentation and Sensory Analysis
- Fungal Biology and Applications
- Ferroelectric and Piezoelectric Materials
- Multiferroics and related materials
- Magnetic and transport properties of perovskites and related materials
- Phytochemicals and Antioxidant Activities
- Biochemical and biochemical processes
- Polysaccharides and Plant Cell Walls
- Entomopathogenic Microorganisms in Pest Control
- Tea Polyphenols and Effects
- Insect symbiosis and bacterial influences
- Plant biochemistry and biosynthesis
- Insect Resistance and Genetics
- Algal biology and biofuel production
- Plant-Microbe Interactions and Immunity
- Pharmacogenetics and Drug Metabolism
- Metal and Thin Film Mechanics
- Ziziphus Jujuba Studies and Applications
- Photosynthetic Processes and Mechanisms
- Nitrogen and Sulfur Effects on Brassica
- Kruppel-like factors research
- Epigenetics and DNA Methylation
Tianjin University of Science and Technology
2020-2024
Yunnan Agricultural University
2016-2022
Tianjin Institute of Industrial Biotechnology
2014-2021
Chinese Academy of Sciences
2013-2021
Tianjin Medical University
2020
Affiliated Hospital of Guizhou Medical University
2019
Guiyang Medical University
2019
Collaborative Innovation Center of Advanced Microstructures
2011-2015
Nanjing University
2011-2015
East China University of Science and Technology
2011-2012
Over the past 3 years, CRISPR/Cas9 system has revolutionized field of genome engineering. However, its application not yet been validated in thermophilic fungi. Myceliophthora thermophila, an important biomass-degrading fungus, attracted industrial interest for production efficient thermostable enzymes. Genetic manipulation is crucial metabolic engineering and to unravel mechanism lignocellulose deconstruction. The lack a powerful, versatile genome-editing tool impeded broader exploitation...
D-glucose, D-xylose and L-arabinose are the three major monosaccharides in plant cell walls. Complete utilization of all sugars is still a bottleneck for second-generation cellulolytic bioethanol production, especially L-arabinose. However, little known about gene expression profiles during fungi comparison genome-wide fungal response to these has not yet been reported.Using next-generation sequencing technology, we have analyzed transcriptome N. crassa grown on versus D-xylose, with...
Fungal pathogens of plants and insects infect their hosts by direct penetration the cuticle. Plant insect cuticles are covered a hydrocarbon-rich waxy outer layer that represents first barrier against infection. However, fungal genes underlie degradation have received little attention. Here we characterize single cytochrome P450 monoxygenase family 52 (MrCYP52) gene pathogen Metarhizium robertsii, demonstrate it encodes an enzyme required for efficient utilization host hydrocarbons....
As well as killing pest insects, the rhizosphere competent insect-pathogenic fungus Metarhizium robertsii also boosts plant growth by providing nitrogenous nutrients and increasing resistance to pathogens. Plant roots secrete abundant but little is known about their utilization spp. mechanistic basis of Metarhizium-plant associations. We report here that M. produces an extracellular invertase (MrInv) on roots. Deletion MrInv (⊿MrInv) reduced sucrose rhizospheric exudates increased...
The multiferroic behaviors of Dy1−xHoxMnO3 are investigated in order to reveal the effects Ho-substitution on independent collinear Dy spin and ferroelectric polarization at low temperature. It is demonstrated that a partial ions significantly suppresses temperature one hand, maintains R-Mn coupling other thus leading remarkable enhancement
The thermophilic filamentous fungus Myceliophthora thermophila has many suitable characteristics for industrial biotechnology and could be a promising new chassis system synthetic biology, particularly the ATCC 42464 strain, whose genome was sequenced in 2011. However, metabolic engineering of this strain using genetic approaches not been reported owing to lack tools organism.In present study, we developed high efficiency Agrobacterium tumefaciens mediated transformation M. thermophila,...
Distilled jujube liquor is an alcoholic beverage made from jujube, which has a unique flavor and sweet taste. The purpose of this study was to explore the effect mixed fermentation on quality distilled by comparing performance between S. cerevisiae, Pichia pastoris Lactobacillus. results showed that there were significant differences in combined strains. Moreover, Lactobacillus increased P. reduced total acid content. E-nose contents methyl, alcohol, aldehyde, ketone substances test bottle...
The effect of nonmagnetic Y partial substitution at the Dy site in Dy1−xYxMnO3 up to x=0.2 on magnetism, specific heat, and ferroelectricity is investigated, which resulted a preliminary multiferroic phase diagram. It revealed that suppresses Dy-spin ordering point (TDy) ferroelectric (TFE) but enhances Mn-spin (TN). interaction between spins Mn remarkably affected by substitution. measured electrical polarization depends complex way because sensitive Mn.
Fungal plant cell wall degradation processes are governed by complex regulatory mechanisms, allowing the organisms to adapt their metabolic program with high specificity available substrates. While uptake of representative mono- and disaccharides is known induce specific transcriptional translational responses, related early signal reception transduction remain largely unkown. A fast reversible way transmission post-translational protein modifications, such as phosphorylations, which could...
Abstract Background Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental fluctuation for their growth competitiveness. In Neurospora crassa , the dual-affinity consists low-affinity transporter GLT-1 two transporters HGT-1/HGT-2, which play diverse roles in transport, carbon metabolism, cellulase expression regulation. However, regulation this dual-transporter response not yet clear. Results study, we report that module...
The cellulolytic fungus Neurospora crassa is considered a potential host for enzyme and bioethanol production. However, large scale applications are hindered by its filamentous growth. Although previous investigations have shown that mycelial morphology in submerged culture can be controlled altering physical factors, there little knowledge available about the control genetic modification.In this study, we screened morphological mutants N. crassa. Of 90 screened, 14 exhibited considerably...
Baijiu is a traditional distilled beverage in China with rich variety of aroma substances. 2,3,5,6-tetramethylpyrazine (TTMP) an important component and has the function promoting cardiovascular cerebrovascular health. During brewing Baijiu, microorganisms jiuqu produce acetoin then synthesize TTMP, but yield TTMP very low. In this work, 2,3-butanediol dehydrogenase (BDH) coding gene BDH1 another BDH2 were deleted or overexpressed to evaluate effect on content Saccharomyces cerevisiae . The...
This study investigates innovative approaches to improve the quality and aroma characteristics of Muscat Hamburg wine production by substituting conventional Saccharomyces cerevisiae yeast with an efficient fermentation strain Schizosaccharomyces pombe. The typical use S. in often leads uniformity prolonged processing times, requiring subsequent malolactic degrade excessive malic acid. advocates for replacement a specific pombe strain, Sp-410, isolated from fermented grains sauce-flavor...
It is essential for microbes to acquire information about their environment. Fungi use soluble degradation products of plant cell wall components understand the substrate composition they grow on. Individual perception pathways have been well described. However, interconnections between remain poorly understood. In present work, we provide evidence crosstalk cellulose and hemicellulose mannan being conserved in several filamentous fungi leading inhibition cellulase expression. We used...
Methanol, which produced in large quantities from low-quality coal and the hydrogenation of CO2, is a potentially renewable one-carbon (C1) feedstock for biomanufacturing. The methylotrophic yeast Pichia pastoris an ideal host methanol biotransformation given its natural capacity as assimilation system. However, utilization efficiency biochemical production limited by toxicity formaldehyde. Therefore, reducing formaldehyde to cells remains challenge engineering design metabolism. Based on...
Aberrant upregulation of hepatic lipogenesis induced by chronic and excessive alcohol consumption is a critical driver the progression alcohol-associated liver disease (ALD), however, no effective approaches inhibiting are currently available for treating ALD patients. Moreover, little known about whether how nonethanol ingredients in alcoholic beverages regulate pathogenesis ALD. Here discovery small molecule that activates production secretion fibroblast growth factor 21 (FGF21) reported....